Patent US4056608A successfully refreshed from Google Patents.
US4056608A
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Cardiac glycoside or aglycone assays
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1976-04-08
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Granted: 1977-11-01
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Expires: 1996-04-08
Abstract
Abstract Immunoassays for digoxin and its congeners are provided by pre-treating the serum sample with dilute aqueous alkaline solutions under mild conditions and for short periods of time, preferably in combination with a chelating agent, prior to carrying out the immunoassay. The method finds particular application with radioimmunoassays and homogeneous enzyme immunoassays.
Inventors
- 👤 Edwin F. Ullman
- 👤 Joyce Y. Chang
- 👤 Kenneth G. McNeil
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 04:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9453; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/825
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
62 words
In an immunoassay for the determination of cardiac glycosides or aglycones employing a serium sample, wherein antibody for cardiac glycosides or aglycones is employed which binds to cardiac glycosides or aglycones in competition with the indicator used for detection, the improvement which comprises: pre-treating said serum sample with an alkaline solution under mild conditions at a pH of at least about 10.5.
Claim 2
Dependent Claim
62 words
In an immunoassay for the determination of cardiac glycosides or aglycones employing a serium sample, wherein antibody for cardiac glycosides or aglycones is employed which binds to cardiac glycosides or aglycones in competition with the indicator used for detection, the improvement which comprises: pre-treating said serum sample with an alkaline solution under mild conditions at a pH of at least about 10.5.
Claim 3
Dependent Claim
54 words
An immunoassay according to claim 1, wherein said pH is in the range of 11 to 13, an aminoalkylene carboxylic acid chelating agent is present in said solution at a concentration in the range of about 0.01 to 0.5M. and said pretreating is at a temperature in the range of 15° to 50° C.
Claim 4
Dependent Claim
12 words
An immunoassay according to claim 2, wherein said immunoassay is a radioimmunoassay.
Claim 5
Dependent Claim
13 words
An immunoassay according to claim 2, wherein said immunoassay is an enzyme immunoassay.
Claim 6
Dependent Claim
12 words
An immunoassay according to claim 4, wherein said enzyme is glucose-6-phosphate dehydrogenase.
Claim 7
Dependent Claim
31 words
An immunoassay according to claim 1, wherein said alkaline treated solution is incubated from about 1 to 60 minutes at a temperature in the range of about 15° to 40° C.
Claim 8
Dependent Claim
12 words
An immunoassay according to claim 6, wherein the determination is for digoxin.
Claim 9
Dependent Claim
12 words
An immunoassay according to claim 6, wherein the determination is for digitoxin.
Claim 10
Dependent Claim
12 words
An immunoassay according to claim 2, wherein said chelating agent is EDTA.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-10-31 | 1981-05-02 | 1981-11-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-10-31 | 1985-05-02 | 1985-11-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-10-31 | 1989-05-02 | 1989-11-01 | $7700.00 | Window Closed / Lapsed |