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US4055633A
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Immunoassay for thymopoietin
Assignee: Memorial Sloan Kettering Cancer Center
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Filing: 1976-07-28
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Granted: 1977-10-25
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Expires: 1996-07-28
Abstract
Abstract An immunoassay for the polypeptidic thymic hormone thymopoietin (thymin) is described. Determination of thymopoietin levels in biological fluids provides a useful diagnostic test for myasthenia gravis (elevated levels), immune deficiency diseases (reduced levels), immunologically mediated diseases such as rheumatoid arthritis, systemic lupus erythematosus and allergy (reduced levels) cancer (reduced levels), malnutrition (reduced levels) and infections (reduced levels). The present assay is also useful as a monitor of the effectiveness of therapy in each of the aforementioned types of disease states.
Inventors
- 👤 Gideon Goldstein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 14:42
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/813; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
18 words
An antigen consisting essentially of thymopoietin covalently bonded to an immunogenic carrier material through a bifunctional linking group.
Claim 2
Dependent Claim
18 words
An antigen consisting essentially of thymopoietin covalently bonded to an immunogenic carrier material through a bifunctional linking group.
Claim 3
Dependent Claim
15 words
The antigen of claim 1 wherein said bifunctional linking group is a C 2-7 dialkanal.
Claim 4
Dependent Claim
12 words
The antigen of claim 2 wherein said C 2-7 dialkanal is glutaraldehyde.
Claim 5
Dependent Claim
13 words
The antigen of claim 1 wherein said immunogenic carrier material is a protein.
Claim 6
Dependent Claim
13 words
The antigen of claim 4 wherein said protein is a mammalian gamma globulin.
Claim 7
Dependent Claim
14 words
The antigen of claim 5 wherein said mammalian gamma globulin is equine gamma globulin.
Claim 8
Dependent Claim
48 words
An antibody specific to thymopoietin and to an antigen consisting essentially of thymopoietin covalently bonded to an immunogenic carrier material through a bifunctional linking group said thymopoietin specific antibody being prepared by innoculating a host animal with the aforesaid antigen and collecting the serum from said host animal.
Claim 9
Dependent Claim
48 words
An antibody specific to thymopoietin and to an antigen consisting essentially of thymopoietin covalently bonded to an immunogenic carrier material through a bifunctional linking group said thymopoietin specific antibody being prepared by innoculating a host animal with the aforesaid antigen and collecting the serum from said host animal.
Claim 10
Dependent Claim
21 words
The antibody of claim 7 wherein said antigen consists of thymopoietin covalently bonded to a protein with a glutaraldehyde linking group.
Claim 11
Dependent Claim
12 words
The antibody of claim 8 wherein said protein is equine gamma globulin.
Claim 12
Dependent Claim
3 words
I 125 -thymopoietin.
Claim 13
Dependent Claim
3 words
I 125 -thymopoietin.
Claim 14
Independent Claim
103 words
A method for the assay of thymopoietin in a sample, which method comprises mixing said sample with a known amount of labeled thymopoietin and an antibody which will selectively complex with said thymopoietin, separating the resulting antibody-antigen complex from the supernatant, measuring the degree of binding of the said labeled thymopoietin in said complex and determining the amount of thymopoietin present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of thymopoietin with fixed amounts of said labeled thymopoietin and said antibody and determining the degree of binding for each known amount of thymopoietin.
Claim 15
Independent Claim
103 words
A method for the assay of thymopoietin in a sample, which method comprises mixing said sample with a known amount of labeled thymopoietin and an antibody which will selectively complex with said thymopoietin, separating the resulting antibody-antigen complex from the supernatant, measuring the degree of binding of the said labeled thymopoietin in said complex and determining the amount of thymopoietin present in said sample by comparing said degree of binding to a standard curve obtained by mixing known amounts of thymopoietin with fixed amounts of said labeled thymopoietin and said antibody and determining the degree of binding for each known amount of thymopoietin.
Claim 16
Dependent Claim
15 words
The method of claim 11 wherein a radioimmunoassay is employed and radiolabeled thymopoietin is used.
Claim 17
Dependent Claim
20 words
The method of claim 12 wherein the antibody-antigen complex is separated from solution with the assistance of 30% polyethylene glycol.
Claim 18
Dependent Claim
37 words
The method of claim 12 wherein the antibody-antigen complex is separated from solution with the assistance of double antibody technique using an antibody against the serum of the host animal in which the thymopoietin antibody was elicited.
Claim 19
Dependent Claim
31 words
The method of claim 12 wherein the antibody-antigen complex is separated from solution with the assistance of a dextran coated charcoal technique wherein the radioactivity of the precipitate represents unbound thymopoietin.
Claim 20
Dependent Claim
13 words
The method of claim 12 wherein said radiolabeled thymopoietin is I 125 -thymopoietin.
Claim 21
Dependent Claim
14 words
The method of claim 12 wherein the sample being assayed is a plasma sample.
Claim 22
Dependent Claim
32 words
Labeled thymopoietin, wherein the label is selected from the group consisting of tritium, carbon 14 , iodine 125 , chromophores, fluorophors, enzymes, red blood cells, latex particles and electron spin resonance groups.
Claim 23
Dependent Claim
32 words
Labeled thymopoietin, wherein the label is selected from the group consisting of tritium, carbon 14 , iodine 125 , chromophores, fluorophors, enzymes, red blood cells, latex particles and electron spin resonance groups.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-10-24 | 1981-04-25 | 1981-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-10-24 | 1985-04-25 | 1985-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-10-24 | 1989-04-25 | 1989-10-25 | $7700.00 | Window Closed / Lapsed |