US3897212A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Test for systemic lupus erythematosus
Assignee: ALBERT EINSTEIN MEDICAL CENTER
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Filing: 1973-01-30
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Granted: 1975-07-29
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Expires: 1993-01-30
Abstract
Abstract A test for systemic lupus erythematosus which involves the detection of anti-DNA antibodies by use of 125I labeled DNA. According to the test, native DNA labeled with 125I labeled 5iododeoxyuridene (IUdR) preferably, is mixed with a sample of serum from a patient to be tested. The mixture is then incubated to allow reaction or complexing of the DNA with any anti-DNA antibodies present in the serum, after which a precipitating agent for antibody/DNA complexes is added and the supernatant and precipitate fractions are separated. The percentage of DNA bound to antibodies may then be readily determined by calculation from the respective radioactivities of the supernatant and precipitate fractions. A high percentage of bound DNA represents a positive test for systemic lupus erythematosus.
Inventors
- 👤 Shalom A Leon
- 👤 Bernard Shapiro
- 👤 George J Kollmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 22:57
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/564; G01N2800/00; G01N2800/10; G01N2800/101; G01N2800/104; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/811
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
134 words
A METHOD FOR TESTING PATIENTS FOR SYSTEMIC LUPUS ERYTHEMATOSUS COMPRISING THE STEPS OF TAKING A SAMPLE OF SERUM FROM A PATIENT TO BE TESTED, LABELING A SAMPLE OF NATIVE DNA WITH 125I BY INTRODUCING 125I-LABELED 5-IODOEXYURIDINE INTO THE DNA MOLECULE, MIXING TOGETHER, IN THE PRESENCE OF A BUFFER, PORTIONS OF SAID 125I-LABELED DNA AND SAID SERUM INCUBATING THE RESULTING MIXTURE TO ALLOW COMPLEXING OF THE DNA WITH ANY ANTI-DNA ANTIBODIES PRESENT IN THE SERUM, ADDING TO SAID MIXTURE A PRECIPITATING AGENT FOR ANTIBODY/DNA COMPLEXES WHICH PRECIPITATING AGENT WILL NOT PRECIPITATE NON-COMPLEXED DNA, SEPARATING THE MIXTURE INTO SUPERNATANT AND PRECIPITATE FRACTIONS, AND DETERMINING THE RESPECTIVE RADIOACTIVITIES OF THE SEPARATED FRACTIONS, A HIGH DEGREE OF RADIOACTIVITY IN THE PRECIPITATE FRACTION INDICATING SUBSTANTIAL COMPLEXING OF DNA WITH ANTIBODIES WHICH REPRESENTS A POSITIVE TEST FOR SYSTEMIC LUPUS ERTHEMATOSUS.
Claim 2
Independent Claim
134 words
A METHOD FOR TESTING PATIENTS FOR SYSTEMIC LUPUS ERYTHEMATOSUS COMPRISING THE STEPS OF TAKING A SAMPLE OF SERUM FROM A PATIENT TO BE TESTED, LABELING A SAMPLE OF NATIVE DNA WITH 125I BY INTRODUCING 125I-LABELED 5-IODOEXYURIDINE INTO THE DNA MOLECULE, MIXING TOGETHER, IN THE PRESENCE OF A BUFFER, PORTIONS OF SAID 125I-LABELED DNA AND SAID SERUM INCUBATING THE RESULTING MIXTURE TO ALLOW COMPLEXING OF THE DNA WITH ANY ANTI-DNA ANTIBODIES PRESENT IN THE SERUM, ADDING TO SAID MIXTURE A PRECIPITATING AGENT FOR ANTIBODY/DNA COMPLEXES WHICH PRECIPITATING AGENT WILL NOT PRECIPITATE NON-COMPLEXED DNA, SEPARATING THE MIXTURE INTO SUPERNATANT AND PRECIPITATE FRACTIONS, AND DETERMINING THE RESPECTIVE RADIOACTIVITIES OF THE SEPARATED FRACTIONS, A HIGH DEGREE OF RADIOACTIVITY IN THE PRECIPITATE FRACTION INDICATING SUBSTANTIAL COMPLEXING OF DNA WITH ANTIBODIES WHICH REPRESENTS A POSITIVE TEST FOR SYSTEMIC LUPUS ERTHEMATOSUS.
Claim 3
Dependent Claim
19 words
A method according to claim 1 wherein the source of DNA to be labeled is growing E. coli cells.
Claim 4
Dependent Claim
20 words
A method according to claim 2 wherein the growing E. coli cells comprise the 15 TAU strain of E. coli.
Claim 5
Dependent Claim
18 words
A method according to claim 1 wherein the source of DNA to be labeled is a thymine-requiring mutant.
Claim 6
Dependent Claim
29 words
A method according to claim 1 wherein only enough 125I-labeled 5-iododeoxyuridine is introduced to yield a final specific radioactivity of the labeled DNA of about 10,000-50,000 cpm/ Mu g.
Claim 7
Dependent Claim
27 words
A method according to claim 1 wherein a percentage of DNA binding to antibodies of greater than 20 percent represents a positive test for systemic lupus erythematosus.
Claim 8
Independent Claim
115 words
A method for testing serum for anti-DNA antibodies comprising the steps of labeling a sample of native DNA with 125I by introducing 125I-labeled 5-iododeoxyuridine into the DNA molecule, mixing said 125I-labeled DNA with a sample of serum to be tested, incubating the resulting mixture to allow reaction of the DNA with any anti-DNA antibodies present in the serum, adding to said mixture a precipitating agent for antibody/DNA complexes which precipitating agent will not precipitate noncomplexed DNA, separating the mixture into supernatant and precipitate fractions, and determining the respective radioactivities of the separated fractions, whereby the percentage of DNA bound to antibodies can be calculated on the basis of the relative radioactivities of the separated fractions.
Claim 9
Independent Claim
115 words
A method for testing serum for anti-DNA antibodies comprising the steps of labeling a sample of native DNA with 125I by introducing 125I-labeled 5-iododeoxyuridine into the DNA molecule, mixing said 125I-labeled DNA with a sample of serum to be tested, incubating the resulting mixture to allow reaction of the DNA with any anti-DNA antibodies present in the serum, adding to said mixture a precipitating agent for antibody/DNA complexes which precipitating agent will not precipitate noncomplexed DNA, separating the mixture into supernatant and precipitate fractions, and determining the respective radioactivities of the separated fractions, whereby the percentage of DNA bound to antibodies can be calculated on the basis of the relative radioactivities of the separated fractions.
Claim 10
Dependent Claim
18 words
A method according to claim 7 wherein the source of DNA to be labeled is a thymine-requiring mutant.
Claim 11
Dependent Claim
22 words
A method according to claim 8 wherein the source of DNA to be labeled comprises the 15 TAU strain of E. coli.
References Cited (Backward Citations)
US3555143A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-07-28 | 1979-01-27 | 1979-07-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-07-28 | 1983-01-27 | 1983-07-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-07-28 | 1987-01-27 | 1987-07-29 | $7700.00 | Window Closed / Lapsed |