Patent US4052504A successfully refreshed from Google Patents.
US4052504A
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Assay for thyroxine binding globulin
Assignee: Corning Glass Works
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Filing: 1976-06-30
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Granted: 1977-10-04
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Expires: 1996-06-30
Abstract
Abstract Method of determining the concentration of thyroxine binding globulin (TBG) in a fluid sample. The method comprises the steps of adding to the sample an excess amount of thyroxine (T 4 ) and then analyzing the sample for T 4 via immunoassay technique in the presence and absence of a blocking agent to establish a binding differential. The differential is then correlated with a standard curve which relates known TBG concentrations to binding differentials obtained in a similar manner.
Inventors
- 👤 William Hertl
- 👤 Gerald Odstrchel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 21:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/804
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
86 words
A method of determining the concentration of thyroxine binding globulin in a sample, the method comprising the steps of 1. adding to the sample an excess amount of thyroxine; 2. analyzing the sample for thyroxine via separate immunoassays, one immunoassay being in the presence of a blocking agent and the other immunoassay being in the absence of a blocking agent to thereby establish a binding differential; and 3. then correlating that differential with a standard curve which relates known thyroxine binding globulin concentrations with binding differentials.
Claim 2
Independent Claim
86 words
A method of determining the concentration of thyroxine binding globulin in a sample, the method comprising the steps of 1. adding to the sample an excess amount of thyroxine; 2. analyzing the sample for thyroxine via separate immunoassays, one immunoassay being in the presence of a blocking agent and the other immunoassay being in the absence of a blocking agent to thereby establish a binding differential; and 3. then correlating that differential with a standard curve which relates known thyroxine binding globulin concentrations with binding differentials.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein the excess thyroxine comprises at least about 200 ng of thyroxine per ml of sample.
Claim 4
Dependent Claim
16 words
The method of claim 1 wherein the immunoassay technique for analyzing for thyroxine is a radioimmunoassay.
Claim 5
Dependent Claim
25 words
The method of claim 1 wherein the radioimmunoassay technique is a solid phase radioimmunoassay technique using silanized glass particles as carriers for the anti-thyroxine antibodies.
Claim 6
Dependent Claim
40 words
The method of claim 1 wherein the binding differential is represented by the difference between the amount of thyroxine bound with and without the use of a blocking agent divided by the amount of thyroxine bound with a blocking agent.
Claim 7
Dependent Claim
14 words
The method of claim 1 wherein the blocking agent is thimerosal or 8-anilino-1-naphthalenesulfonic acid.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-10-03 | 1981-04-04 | 1981-10-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-10-03 | 1985-04-04 | 1985-10-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-10-03 | 1989-04-04 | 1989-10-04 | $7700.00 | Window Closed / Lapsed |