US3929981A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for determining thyroid function
Assignee: ER Squibb and Sons LLC
•
Filing: 1974-02-27
•
Granted: 1975-12-30
•
Expires: 1994-02-27
Abstract
Abstract In an in vitro test for the determination of thyroxine using the competitive protein-binding technique, the improvement that comprises extracting the thyroxine into a lower alkanol or dioxane, but not separating the lower alkanol or dioxane solution of thyroxine from the serum prior to measurement of the thyroxine.
Inventors
- 👤 Hari S Murty
- 👤 Siva K Gara
- 👤 Dasika R Murty
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 17:26
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/825
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
95 words
IN A METHOD OF DETERMING THYROXINE IN SERUM BY THE COMPETITIVE PROTEIN-BINDING TECHNIQUE COMPRISING EXTRACTING THYROXINE FROM THE SERUM WITH A LOWER ALKANOL OR DIOXANE SOLVENT; ADDING TO THE SERUM AND SOLVENT A SOLUTION OF THYROXINE-BINDING GLOBULIN SATURATED WITH RADIOISOTOPE LABELLED THYROXINE; SEPARATING THYROXINE THAT IS BOUND TO THE THYROXINEBINDING GLOBULIN FROM UNBOUND THYROXINE; AND COUNTING THE BOUND THYROXINE, THE UNBOUND THYROXINE, OR BOTH BOUND AND UNBOUND THYROXINE; THE IMPROVEMENT WHEREIN: THE SOLVENT EXTRACT IS NOT SEPARATED FROM THE SERUM PRIOR TO THE ADDITION OF THE SOLUTION OF THYROXINE-BINDING GLOBULIN SATURATED WITH RADIOISOTOPE LABELLED THYROXINE.
Claim 2
Dependent Claim
95 words
IN A METHOD OF DETERMING THYROXINE IN SERUM BY THE COMPETITIVE PROTEIN-BINDING TECHNIQUE COMPRISING EXTRACTING THYROXINE FROM THE SERUM WITH A LOWER ALKANOL OR DIOXANE SOLVENT; ADDING TO THE SERUM AND SOLVENT A SOLUTION OF THYROXINE-BINDING GLOBULIN SATURATED WITH RADIOISOTOPE LABELLED THYROXINE; SEPARATING THYROXINE THAT IS BOUND TO THE THYROXINEBINDING GLOBULIN FROM UNBOUND THYROXINE; AND COUNTING THE BOUND THYROXINE, THE UNBOUND THYROXINE, OR BOTH BOUND AND UNBOUND THYROXINE; THE IMPROVEMENT WHEREIN: THE SOLVENT EXTRACT IS NOT SEPARATED FROM THE SERUM PRIOR TO THE ADDITION OF THE SOLUTION OF THYROXINE-BINDING GLOBULIN SATURATED WITH RADIOISOTOPE LABELLED THYROXINE.
Claim 3
Dependent Claim
14 words
The process in accordance with claim 1 wherein the solvent is a lower alkanol.
Claim 4
Dependent Claim
12 words
The process in accordance with claim 2 wherein the solvent is ethanol.
Claim 5
Dependent Claim
12 words
The process in accordance with claim 1 wherein the solvent is dioxane.
Claim 6
Dependent Claim
20 words
The process in accordance with claim 1 wherein the volume ratio of serum to solvent is from 3:1 to 1:1.
Claim 7
Dependent Claim
20 words
The process in accordance with claim 1 wherein the volume ratio of serum to solvent is from 2:1 to 1:1.
Claim 8
Dependent Claim
18 words
The process in accordance with claim 1 wherein the volume ratio of serum to solvent is about 1.4:1.
Claim 9
Dependent Claim
26 words
The process in accordance with claim 1 wherein the thyroxine bound to the thyroxine-binding globulin is separated from the unbound thyroxine using an anion exchange material.
Claim 10
Dependent Claim
16 words
The process in accordance with claim 1 wherein the radioisotope labelled thyroxine is labelled with I-125.
Claim 11
Dependent Claim
110 words
The process for determining the thyroxine content of blood serum using the competitive protein-binding technique which comprises: i. extracting thyroxine from a serum sample by adding a lower alkanol or dioxane solvent to the serum; ii. adding a solution of thyroxine-binding globulin saturated with radioisotope labelled thyroxine to the mixture of serum and solvent; iii. separating the thyroxine that is bound to the thyroxine-binding globulin from the unbound thyroxine by adding an adsorbent to the mixture and separating the solid material from the liquid; and iv. measuring the radioactivity on the adsorbent, the radioactivity in the liquid, or both the radioactivity on the adsorbent and the radioactivity in the liquid.
Claim 12
Dependent Claim
110 words
The process for determining the thyroxine content of blood serum using the competitive protein-binding technique which comprises: i. extracting thyroxine from a serum sample by adding a lower alkanol or dioxane solvent to the serum; ii. adding a solution of thyroxine-binding globulin saturated with radioisotope labelled thyroxine to the mixture of serum and solvent; iii. separating the thyroxine that is bound to the thyroxine-binding globulin from the unbound thyroxine by adding an adsorbent to the mixture and separating the solid material from the liquid; and iv. measuring the radioactivity on the adsorbent, the radioactivity in the liquid, or both the radioactivity on the adsorbent and the radioactivity in the liquid.
Claim 13
Dependent Claim
14 words
The process in accordance with claim 10 wherein the solvent is a lower alkanol.
Claim 14
Dependent Claim
13 words
The process in accordance with claim 11 wherein the lower alkanol is ethanol.
Claim 15
Dependent Claim
12 words
The process in accordance with claim 10 wherein the solvent is dioxane.
Claim 16
Dependent Claim
15 words
The process in accordance with claim 10 wherein the adsorbent is an anion exchange material.
Claim 17
Dependent Claim
16 words
The process in accordance with claim 10 wherein the radioisotope labelled thyroxine is labelled with I-125.
Claim 18
Dependent Claim
20 words
The process in accordance with claim 10 wherein the volume ratio of serum to solvent is from 3:1 to 1:1.
Claim 19
Dependent Claim
20 words
The process in accordance with claim 10 wherein the volume ratio of serum to solvent is from 2:1 to 1:1.
Claim 20
Dependent Claim
18 words
The process in accordance with claim 10 wherein the volume ratio of serum to solvent is about 1.4:1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-29 | 1979-06-30 | 1979-12-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-29 | 1983-06-30 | 1983-12-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-29 | 1987-06-30 | 1987-12-30 | $7700.00 | Window Closed / Lapsed |