Patent US4038143A successfully refreshed from Google Patents.
US4038143A
Expired / Lapsed
US
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Test kit for the genetic detection of microorganisms
Assignee: University of Michigan (System)
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Filing: 1975-08-15
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Granted: 1977-07-26
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Expires: 1995-08-15
Abstract
Abstract A test kit for detecting the presence of a specific microorganism in a biological sample utilizing the phenomenon of genetic transformation. The test kit comprises a transformable variant of said specific microorganism, such as auxotrophs which are distinguishable from their wild parent strain. The variants are transformed by deoxyribonucleic acid (DNA) extracted from microorganism cells present in a biological test sample. The detection of transformed cells indicates the presence of the specific microorganism under determination in the sample. The microorganism cells present in the biological sample are lysed utilizing an aqueous solution containing a surfactant capable of performing the lysing step.
Inventors
- 👤 Elliot Juni
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 04:01
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12N; C12N15/00; C12Q1/02; C12Q1/04; C12Q1/12; C12Q1/14; C12Q1/6806; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/851; Y10S435/871; Y10S435/882; Y10S435/885
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
104 words
A test kit for detecting the presence of a specific microorganism in a biological sample based on the phenomenon of genetic transformation, which test kit comprises (1) a transformable variant of said specific microorganism which is distinguishable therefrom and (2) an aqueous solution separate from said variant containing a surfactant at a concentration of between about 0.01 and 0.1 percent by weight which is sufficient to lyse substantially all of the microorganism cells present in said biological sample while insufficient to destroy a substantial amount of said variant upon contact therewith when said variant is supported on a medium capable of diffusing aqueous solutions.
Claim 2
Dependent Claim
104 words
A test kit for detecting the presence of a specific microorganism in a biological sample based on the phenomenon of genetic transformation, which test kit comprises (1) a transformable variant of said specific microorganism which is distinguishable therefrom and (2) an aqueous solution separate from said variant containing a surfactant at a concentration of between about 0.01 and 0.1 percent by weight which is sufficient to lyse substantially all of the microorganism cells present in said biological sample while insufficient to destroy a substantial amount of said variant upon contact therewith when said variant is supported on a medium capable of diffusing aqueous solutions.
Claim 3
Dependent Claim
14 words
A test kit as in claim 1 wherein said surfactant is sodium dodecyl sulfate.
Claim 4
Dependent Claim
21 words
A test kit as in claim 1 which additionally includes a medium capable of supporting said variant and diffusing aqueous solutions.
Claim 5
Dependent Claim
12 words
A test kit as in claim 3 wherein said medium is gelatinous.
Claim 6
Dependent Claim
19 words
A test kit as in claim 3 wherein said medium is a microbiological growth supporting medium for said variant.
Claim 7
Dependent Claim
12 words
A test kit as in claim 5 wherein said medium is gelatinous.
Claim 8
Dependent Claim
43 words
A test kit as in claim 3 which additionally includes a second medium capable of eliciting a response to either the presence or absence of a characteristic that distinguishes said specific microorganism from said variant upon contact with a prototroph of said variant.
Claim 9
Dependent Claim
13 words
A test kit as in claim 7 wherein said response is microbiological growth.
Claim 10
Dependent Claim
29 words
A test kit as in claim 8 wherein said second medium contains an indicator which yields a colorimetric response to either the presence or absence of said microbiological growth.
Claim 11
Dependent Claim
15 words
A test kit as in claim 1 wherein said variant is in a suspended state.
Claim 12
Dependent Claim
15 words
A test kit as in claim 10 wherein said variant is in a lyophilized state.
Claim 13
Dependent Claim
13 words
A test kit as in claim 1 wherein said variant is an auxotroph.
Claim 14
Dependent Claim
22 words
A test kit as in claim 12 which additionally includes a medium capable of supporting said variant and of diffusing aqueous solutions.
Claim 15
Dependent Claim
12 words
A test kit as in claim 13 wherein said medium is gelatinous.
Claim 16
Dependent Claim
32 words
A test kit as in claim 13 which additionally includes a microbiological growth supporting medium for said specific microorganism, which medium lacks at least one nutrient required by said variant for growth.
Claim 17
Dependent Claim
15 words
A test kit as in claim 15 wherein said microbiological growth support medium is gelatinous.
Claim 18
Dependent Claim
26 words
A test kit as in claim 1 wherein said specific microorganism to be detected is a Moraxella, Pheumococcus, Haemophilus, Streptococcus, Staphylococcus, Bacillus, Neisseria, Rhizorium or Acinetobacter.
Claim 19
Dependent Claim
14 words
A test kit as in claim 1 wherein said specific microorganism is a Moraxella.
Claim 20
Dependent Claim
14 words
A test kit as in claim 1 wherein said specific microorganism is an Acinetobacter.
Claim 21
Dependent Claim
14 words
A test kit as in claim 1 wherein said specific microorganism is a Neisseria.
Claim 22
Dependent Claim
14 words
A test kit as in claim 1 wherein said specific microorganism is Neisseria gonnorrhoeae.
References Cited (Backward Citations)
US3671400A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-07-25 | 1981-01-24 | 1981-07-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-07-25 | 1985-01-24 | 1985-07-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-07-25 | 1989-01-24 | 1989-07-26 | $7700.00 | Window Closed / Lapsed |