Syntheses of 3,24R,25- and 3,24S,25-trihydroxy-5,7-cholestadiene 24,25-ketals and alkanoyl derivatives thereof

Claim 2

A compound of the formula ##STR14## wherein R 1 is hydroxy or lower alkanoyloxy, R 2 and R 3 each independently are lower alkyl, R 2 and R 3 taken together are lower alkylene, X is bromo or chloro and the absolute configuration at C-24 is R or S.

Claim 3

The compound of claim 1 wherein R 1 is lower alkanoyloxy and R 2 and R 3 each independently are lower alkyl.

Claim 4

The compound of claim 2 which is 7-bromo-24R,25-dihydroxycholesteryl 3-acetate 24,25-acetonide.

Claim 5

The compound of claim 2 which is 7-bromo-24S,25-dihydroxycholesteryl 3-acetate 24,25-acetonide.

Claim 6

A compound of the formula ##STR15## wherein R 1 is hydroxy or lower alkanoyloxy, R 2 and R 3 each independently are lower alkyl, R 2 and R 3 taken together are lower alkylene and the absolute configuration at C-24 is R or S.

Claim 7

A compound of the formula ##STR15## wherein R 1 is hydroxy or lower alkanoyloxy, R 2 and R 3 each independently are lower alkyl, R 2 and R 3 taken together are lower alkylene and the absolute configuration at C-24 is R or S.

Claim 8

The compound of claim 5 wherein R 1 is hydroxy or lower alkanoyloxy and R 2 and R 3 each independently are lower alkyl.

Claim 9

The compound of claim 6 which is 3,24R,25-trihydroxy-5,7-cholestadiene 3-acetate 24,25-acetonide.

Claim 10

The compound of claim 6 which is 3,24S,25-trihydroxy-5,7-cholestadiene 3-acetate 24,25-acetonide.

Claim 11

The compound of claim 6 which is 3,24R,25-trihydroxy-5,7-cholestadiene 24,25-acetonide.

Claim 12

The compound of claim 6 which is 3,24S,25-trihydroxy-5,7-cholestadiene 24,25-acetonide.

Claim 13

A process for the preparation of 3,24,25-trihydroxy-5,7-cholestadiene 24,25-ketal and the alkanoyl derivative thereof of the formula ##STR16## wherein R 1 is hydroxy or lower alkanoyloxy, R 2 and R 3 each independently are lower alkyl, R 2 and R 3 taken together are lower alkylene and the absolute configuration at C-24 is R or S which comprises the steps of (a) treating a compound of the formula ##STR17## wherein R 1 , R 2 and R 3 are as above with a chlorinating or brominating agent in an inert organic solvent to form a compound of the formula ##STR18## wherein R 1 , R 2 and R 3 are as above, X is chloro or bromo and the absolute configuration at C-24 is R or S (b) treating said product from step (a) with a heteroaromatic or aliphatic tertiary amine in an inert solvent to form a mixture of 3,24,25-trihydroxy-5,7-cholestadiene 24,25-ketal and the alkanoyl derivative thereof and a compound of the formula ##STR19## wherein R 1 , R 2 and R 3 are as above and the absolute configuration at C-24 is R or S (c) treating the mixture with an organic acid selected from the group consisting of sulfuric acid and sulfonic acids in a second inert organic solvent to form a second mixture of 3,24,25-trihydroxy-5,7-colestadiene 24,25-ketal and the alkanoyl derivative thereof and a compound of the formula ##STR20## wherein R 2 and R 3 are as above and (d) separating the mixture to obtain pure 3,24,25-trihydroxy-5,7-cholestadiene 24,25-ketal and the alkanoyl derivative thereof.

Claim 14

A process for the preparation of 3,24,25-trihydroxy-5,7-cholestadiene 24,25-ketal and the alkanoyl derivative thereof of the formula ##STR16## wherein R 1 is hydroxy or lower alkanoyloxy, R 2 and R 3 each independently are lower alkyl, R 2 and R 3 taken together are lower alkylene and the absolute configuration at C-24 is R or S which comprises the steps of (a) treating a compound of the formula ##STR17## wherein R 1 , R 2 and R 3 are as above with a chlorinating or brominating agent in an inert organic solvent to form a compound of the formula ##STR18## wherein R 1 , R 2 and R 3 are as above, X is chloro or bromo and the absolute configuration at C-24 is R or S (b) treating said product from step (a) with a heteroaromatic or aliphatic tertiary amine in an inert solvent to form a mixture of 3,24,25-trihydroxy-5,7-cholestadiene 24,25-ketal and the alkanoyl derivative thereof and a compound of the formula ##STR19## wherein R 1 , R 2 and R 3 are as above and the absolute configuration at C-24 is R or S (c) treating the mixture with an organic acid selected from the group consisting of sulfuric acid and sulfonic acids in a second inert organic solvent to form a second mixture of 3,24,25-trihydroxy-5,7-colestadiene 24,25-ketal and the alkanoyl derivative thereof and a compound of the formula ##STR20## wherein R 2 and R 3 are as above and (d) separating the mixture to obtain pure 3,24,25-trihydroxy-5,7-cholestadiene 24,25-ketal and the alkanoyl derivative thereof.

Claim 15

The process of claim 11 wherein the heteroaromatic amine is an alkylpyridine.

Claim 16

The process of claim 12 wherein the alkylpyridine is s-collidine.

Claim 17

The process of claim 11 wherein the inert organic solvent is an aromatic solvent.

Claim 18

The process of claim 14 wherein the aromatic solvent is xylene.

Claim 19

A process for the preparation of a compound of the formula ##STR21## wherein R 2 and R 3 each independently are lower alkyl, R 2 and R 3 taken together are lower alkylene and the absolute configuration at C-24 is R or S which comprises treating a compound of the formula ##STR22## wherein the absolute configuration at C-24 is R or S with a compound of the formula ##STR23## wherein R 2 and R 3 are as above, R 6 and R 7 each independently are lower alkyl and R 6 and R 7 taken together are lower alkylene in the presence of a strong acid in an inert solvent at a reduced temperature.

Claim 20

A process for the preparation of a compound of the formula ##STR21## wherein R 2 and R 3 each independently are lower alkyl, R 2 and R 3 taken together are lower alkylene and the absolute configuration at C-24 is R or S which comprises treating a compound of the formula ##STR22## wherein the absolute configuration at C-24 is R or S with a compound of the formula ##STR23## wherein R 2 and R 3 are as above, R 6 and R 7 each independently are lower alkyl and R 6 and R 7 taken together are lower alkylene in the presence of a strong acid in an inert solvent at a reduced temperature.

Claim 21

The process of claim 16 wherein R 2 and R 3 each independently are lower alkyl.

Claim 22

The process of claim 16 wherein the strong acid is a sulfonic acid.

Claim 23

The process of claim 18 wherein the strong acid is para-toluenesulfonic acid.

Claim 24

The process of claim 16 wherein the inert solvent is an organic solvent.

Claim 25

The process of claim 20 wherein the organic solvent is acetone.

Claim 26

The process of claim 20 wherein the organic solvent is methanol.

Claim 27

The process of claim 16 wherein the reaction temperature is between about -20° C. and +20° C.

Claim 28

The process of claim 23 wherein the reaction temperature is about 0° C.

Claim 29

The process of claim 11 wherein the sulfonic acid is p-toluenesulfonic acid.