Composition and method of using a protein mixture derived from liver

Claim 2

A composition comprising (a) a pharmaceutically compatible excipient, (b) an inflammation inhibiting amount of corticosteroid, and (c) a biologically effective amount for the treatment of disease in mammals of protein mixture for enhancing metabolic functions derived by: 1 treating liver with an aqueous solution of divalent metal activating cation at a pH essentially neutral or slightly alkaline and separating the resultant solids from the liquid; 2.

Claim 3

treating the liquid with heavy metal precipitating agent and separating the resultant precipitate from the liquid; 3. adjusting the pH of the liquid from step (2) to a range of between 8.3 and 8.5 and separating the resultant precipitate from the liquid; 4. treating the liquid from step (3) with a concentrated solution of salting out agent to precipitate undesired protein and separating the protein precipitate from the liquid; 5. adding solvent to the liquid from step (4) in an amount sufficient to give the resultant mixture a solvent concentration of 20 to 26% by volume and separating the resultant precipitate from the liquid; adding solvent to the liquid in an amount sufficient to give the resultant mixture a solvent concentration to 35 to 40% by volume to produce a mixture having three layers; separating the upper layer and adding to it in amount sufficient to give the resultant mixture a solvent content 70 and 77% by volume whereby protein is precipitated; 6. separating the protein precipitate and dispersing it in a solvent at a pH of 7.8 to 8.2; 7. dialyzing the dispersion from step (6); 8. heating the dialyzate from step (7) to a temperature between 46 and 50° C. and separating the resultant precipitate from the liquid; 9. subjecting the liquid from step (8) to electrophoresis and recovering a

Claim 4

treating the liquid with heavy metal precipitating agent and separating the resultant precipitate from the liquid; 3. adjusting the pH of the liquid from step (2) to a range of between 8.3 and 8.5 and separating the resultant precipitate from the liquid; 4. treating the liquid from step (3) with a concentrated solution of salting out agent to precipitate undesired protein and separating the protein precipitate from the liquid; 5. adding solvent to the liquid from step (4) in an amount sufficient to give the resultant mixture a solvent concentration of 20 to 26% by volume and separating the resultant precipitate from the liquid; adding solvent to the liquid in an amount sufficient to give the resultant mixture a solvent concentration to 35 to 40% by volume to produce a mixture having three layers; separating the upper layer and adding to it in amount sufficient to give the resultant mixture a solvent content 70 and 77% by volume whereby protein is precipitated; 6. separating the protein precipitate and dispersing it in a solvent at a pH of 7.8 to 8.2; 7. dialyzing the dispersion from step (6); 8. heating the dialyzate from step (7) to a temperature between 46 and 50° C. and separating the resultant precipitate from the liquid; 9. subjecting the liquid from step (8) to electrophoresis and recovering a

Claim 5

biologically active protein mixture. 2. A method of treating disease conditions in the group consisting of cirrhosis of the liver, scar tissue, tissue damage, wounds and arthritis comprising administering a therapeutically effective amount of protein mixture derived by: 1. treating liver with an aqueous solution of divalent metal activating cation at a pH essentially neutral or slightly alkaline and separating the resultant solids from the liquid; 2. treating the liquid with heavy metal precipitating agent and separating the resultant precipitate from the liquid; 3. adjusting the pH of the liquid from step (2) to a range of between 8.3 and 8.5 and separating the resultant precipitate from the liquid; 4. treating the liquid from step (3) with a concentrated solution of salting out agent to precipitate undesired protein and separating the protein precipitate from the liquid; 5. adding solvent to the liquid from step (4) in an amount sufficient to give the resultant mixture a solvent concentration of 20 to 26% by volume and separating the resultant precipitate from the liquid; adding solvent to the liquid in an amount sufficient to give the resultant mixture a solvent concentration to 35 to 40% by volume to produce a mixture having three layers; separating the upper layer and adding to it in amount sufficient to give the resultant mixture a solvent content 70 and 77% by volume whereby protein is precipitated; 6. separating the protein precipitate and dispersing it in a solvent at a pH of 7.8 to 8.2; 7. dialyzing the dispersion from step (6); 8. heating the dialyzate from step (7) to a temperature between 46° and 50° C. and separating the resultant precipitate from the liquid; 9. subjecting the liquid from step (8) to electrophoresis and recovering a biologically active protein mixture.

Claim 6

biologically active protein mixture. 2. A method of treating disease conditions in the group consisting of cirrhosis of the liver, scar tissue, tissue damage, wounds and arthritis comprising administering a therapeutically effective amount of protein mixture derived by: 1. treating liver with an aqueous solution of divalent metal activating cation at a pH essentially neutral or slightly alkaline and separating the resultant solids from the liquid; 2. treating the liquid with heavy metal precipitating agent and separating the resultant precipitate from the liquid; 3. adjusting the pH of the liquid from step (2) to a range of between 8.3 and 8.5 and separating the resultant precipitate from the liquid; 4. treating the liquid from step (3) with a concentrated solution of salting out agent to precipitate undesired protein and separating the protein precipitate from the liquid; 5. adding solvent to the liquid from step (4) in an amount sufficient to give the resultant mixture a solvent concentration of 20 to 26% by volume and separating the resultant precipitate from the liquid; adding solvent to the liquid in an amount sufficient to give the resultant mixture a solvent concentration to 35 to 40% by volume to produce a mixture having three layers; separating the upper layer and adding to it in amount sufficient to give the resultant mixture a solvent content 70 and 77% by volume whereby protein is precipitated; 6. separating the protein precipitate and dispersing it in a solvent at a pH of 7.8 to 8.2; 7. dialyzing the dispersion from step (6); 8. heating the dialyzate from step (7) to a temperature between 46° and 50° C. and separating the resultant precipitate from the liquid; 9. subjecting the liquid from step (8) to electrophoresis and recovering a biologically active protein mixture.

Claim 7

A method of facilitating tissue repair in mammals comprising administering a therapeutically effective amount of the composition of claim 2.

Claim 8

A method of treating cirrhosis of the liver in mammals comprising administering a therapeutically effective amount of the composition of claim 2.

Claim 9

A method of treating scar tissue in mammals comprising administering a therapeutically effective amount of the composition of claim 2.

Claim 10

A method of treating arthritis in mammals comprising administering a therapeutically effective amount of the composition of claim 2.

Claim 11

A method of simultaneously inhibiting inflammation and facilitating wound healing in mammals comprising administering a therapeutically effective amount of the composition of claim 2.