US3876774A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of using a simple protein derived from liver
Assignee: Palolab Pharmaceuticals Corp
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Filing: 1971-12-27
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Granted: 1975-04-08
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Expires: 1991-12-27
Abstract
Abstract A new simple protein, described in a copending application, is useful in treating several disease states by enhancing metabolism functions. The protein facilitates glucose uptake in muscle as well as glycogen deposition, leading to utility in the treatment of uremia and shock in animals.
Inventors
- 👤 Jack G Fortini
- 👤 Walter A Blair
- 👤 Gerald M Grodsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 12:44
CPC Classifications
C; C07; C07K; C07K2/00; A; A61; A61K; A61K35/00; A61K35/12; A61K35/37; A61K35/407; A61K38/00
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
35 words
A COMPOSITION FOR THE TREATMENT OF DISEASE IN MAMMALS COMPRISING (A) A PHARMACEUTICALLY COMPATIBLE EXCIPIENT AND (B) PROTEIN IN AN AMOUNT EFFECTIVE FOR ENHANCING METABOLIC FUNTIONS, SAID PROTEIN BEING A BIOLOGICALLY ACTIVE PROTEIN DERIVED BY;
Claim 2
Dependent Claim
35 words
A COMPOSITION FOR THE TREATMENT OF DISEASE IN MAMMALS COMPRISING (A) A PHARMACEUTICALLY COMPATIBLE EXCIPIENT AND (B) PROTEIN IN AN AMOUNT EFFECTIVE FOR ENHANCING METABOLIC FUNTIONS, SAID PROTEIN BEING A BIOLOGICALLY ACTIVE PROTEIN DERIVED BY;
Claim 3
Dependent Claim
27 words
TREATING LIVER WITH AN AQUEOUS SOLUTION OF DIVALENT METAL ACTIVATING CATION AT A PH ESSENTIALLY NEUTRAL OR SLIGHTLY ALKALINE AND SEPARATING THE RESULTANT SOLIDS FROM THE LIQUID;
Claim 4
Dependent Claim
27 words
TREATING LIVER WITH AN AQUEOUS SOLUTION OF DIVALENT METAL ACTIVATING CATION AT A PH ESSENTIALLY NEUTRAL OR SLIGHTLY ALKALINE AND SEPARATING THE RESULTANT SOLIDS FROM THE LIQUID;
Claim 5
Dependent Claim
15 words
TREATING THE LIQUID WITH HEAVY METAL PRECIPTATING AGENT AND SEPARATING CARESULTANT PRECIPITATE FROM THE LIQUID;
Claim 6
Dependent Claim
15 words
TREATING THE LIQUID WITH HEAVY METAL PRECIPTATING AGENT AND SEPARATING CARESULTANT PRECIPITATE FROM THE LIQUID;
Claim 7
Dependent Claim
19 words
A method of treating an inflammatory condition comprising injecting a therapeutically effective amount of the composition of claim 1.
Claim 8
Dependent Claim
17 words
A method of treating shock comprising injecting a therapeutically effective amount of the composition of claim 1.
Claim 9
Dependent Claim
25 words
ADJUSTING THE PH OF THE LIQUID FROM STEP (2) TO A RANGE OF BETWEEN 8.3 AND 8.5 AND SEPARATING THE RESULTANT PRECIPITATE FROM THE LIQUID;
Claim 10
Dependent Claim
25 words
ADJUSTING THE PH OF THE LIQUID FROM STEP (2) TO A RANGE OF BETWEEN 8.3 AND 8.5 AND SEPARATING THE RESULTANT PRECIPITATE FROM THE LIQUID;
Claim 11
Dependent Claim
26 words
TREATING THE LIQUID FROM STEP (3) WITH A CONCENTRATED SOLUTION OF SALTING OUT AGENT TO PRECIPITATE UNDESIRED PROTEIN AND SEPARATING THE PROTEIN PRECIPITATE FROM THE LIQUID;
Claim 12
Dependent Claim
26 words
TREATING THE LIQUID FROM STEP (3) WITH A CONCENTRATED SOLUTION OF SALTING OUT AGENT TO PRECIPITATE UNDESIRED PROTEIN AND SEPARATING THE PROTEIN PRECIPITATE FROM THE LIQUID;
Claim 13
Dependent Claim
17 words
A method of treating uremia comprising injecting a therapeutically effective amount of the composition of claim 1.
Claim 14
Dependent Claim
17 words
A method of treating hepatitis comprising injecting a therapeutically effective amount of the composition of claim 1.
Claim 15
Dependent Claim
92 words
ADDING SOLVENT TO THE LIQUID FROM STEP (4) IN AN AMOUNT SUFFICIENT TO GIVE THE RESULTANT MIXTURE A SOLVENT CONCENTRATION OF 20 TO 26% BY VOLUME AND SEPARATING THE RESULTANT PRECIPITATE FROM THE LIQUID; ADDING SOLVENT TO THE LIQUID IN AN AMOUNT SUFFICIENT TO GIVE THE RESULTANT MIXTURE A SOLVENT CONCENTRATION TO 35 TO 40% BY VOLUME TO PRODUCE A MIXTURE HAVING THREE LAYERS; SEPARATING THE UPPER LAYER AND ADDING TO IT AN AMOUNT SUFFICIENT TO GIVE THE RESULTANT MIXTURE A SOLVENT CONTENT 70 AND 77% BY VOLUME WHEREBY PROTEIN IS PRECIPITATED;
Claim 16
Dependent Claim
92 words
ADDING SOLVENT TO THE LIQUID FROM STEP (4) IN AN AMOUNT SUFFICIENT TO GIVE THE RESULTANT MIXTURE A SOLVENT CONCENTRATION OF 20 TO 26% BY VOLUME AND SEPARATING THE RESULTANT PRECIPITATE FROM THE LIQUID; ADDING SOLVENT TO THE LIQUID IN AN AMOUNT SUFFICIENT TO GIVE THE RESULTANT MIXTURE A SOLVENT CONCENTRATION TO 35 TO 40% BY VOLUME TO PRODUCE A MIXTURE HAVING THREE LAYERS; SEPARATING THE UPPER LAYER AND ADDING TO IT AN AMOUNT SUFFICIENT TO GIVE THE RESULTANT MIXTURE A SOLVENT CONTENT 70 AND 77% BY VOLUME WHEREBY PROTEIN IS PRECIPITATED;
Claim 17
Dependent Claim
17 words
SEPARATING THE PROTEIN PRECIPITATE AND DISPERSING IT IN A SOLVENT AT A PH OF 7.8 TO 8.2;
Claim 18
Dependent Claim
17 words
SEPARATING THE PROTEIN PRECIPITATE AND DISPERSING IT IN A SOLVENT AT A PH OF 7.8 TO 8.2;
Claim 19
Dependent Claim
28 words
A method of increasing the rate of urea nitrogen synthesis in mammalian liver comprising injecting a therapeutically effective amount of the composition of claim 1 into the mammal.
Claim 20
Dependent Claim
21 words
A method of reducing peripheral blood ammonia in mammals comprising injecting a therapeutically effective amount of the composition of claim 1.
Claim 21
Dependent Claim
6 words
DIALYZING THE DISPERSION FROM STEP (6);
Claim 22
Dependent Claim
6 words
DIALYZING THE DISPERSION FROM STEP (6);
Claim 23
Dependent Claim
21 words
HEATING THE DIALYZATE FROM STEP (7) TO A TEMPERATURE BETWEEN 46* AND 50*C. AND SEPARATING THE RESULTANT PRECIPITATE FROM THE LIQUID;
Claim 24
Dependent Claim
21 words
HEATING THE DIALYZATE FROM STEP (7) TO A TEMPERATURE BETWEEN 46* AND 50*C. AND SEPARATING THE RESULTANT PRECIPITATE FROM THE LIQUID;
Claim 25
Dependent Claim
28 words
A method of simultaneously facilitating glucose uptake and glycogen deposition in mammalian cells comprising injecting a therapeutically effctive amount of the composition of claim 1 into the mammal.
Claim 26
Dependent Claim
14 words
SUBJECTING THE LIQUID FROM STEP (8) TO ELECTROPHORESIS AND RECOVERING A BIOLOGICALLY ACTIVE PROTEIN.
Claim 27
Dependent Claim
14 words
SUBJECTING THE LIQUID FROM STEP (8) TO ELECTROPHORESIS AND RECOVERING A BIOLOGICALLY ACTIVE PROTEIN.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-07 | 1978-10-07 | 1979-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-07 | 1982-10-07 | 1983-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-07 | 1986-10-07 | 1987-04-08 | $7700.00 | Window Closed / Lapsed |