Patent US4022876A successfully refreshed from Google Patents.
US4022876A
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Mass spectrometric immunoassay
Assignee: SRI International Inc
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Filing: 1973-06-21
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Granted: 1977-05-10
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Expires: 1993-06-21
Abstract
Abstract A method and means of making an immunological assay is provided whereby stable isotopes of certain elements, or long-lived radioisotopes of these elements are used to tag antigens or antibodies. A known amount of the tagged antibodies or antigens are then mixed with the unknown number of antigens or antibodies forming an antigen-antibody complex, (bound) and free tagged antigens, or antibodies, (unbound). The bound antigens or antibodies are separated from the unbound, and their quantity is determined by negative ion mass spectrometry. An aliquot of the unbound or bound antigens or antibodies is taken and dried in a metal crucible, for example, which is then inserted into a negative ion mass spectrometer where the sample is vaporized, ionized and the number of tagging atoms is then counted. From the information provided by the count, an immunoassay can be established.
Inventors
- 👤 Michael Anbar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 21:05
CPC Classifications
G; G21; G21H; G21H5/00; G21H5/02; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/806; Y10S436/807
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
108 words
The method of making an immunological assay of a sample contining an unknown amount of an antigen or antibody comprising: preparing a solution containing a known amount of one of said antigens or antibodies, when the sample contains the other, tagged with a stable isotope or a long lived radioisotope, mixing said sample with said solution to produce a mixture of tagged bound and unbound antigens or antibodies, separating said bound from said unbound, and applying said bound or said unbound to a negative ion mass spectrometer for counting the number of ions of tagging material whereby the amount of antigens or antibodies in said sample are determined.
Claim 2
Independent Claim
108 words
The method of making an immunological assay of a sample contining an unknown amount of an antigen or antibody comprising: preparing a solution containing a known amount of one of said antigens or antibodies, when the sample contains the other, tagged with a stable isotope or a long lived radioisotope, mixing said sample with said solution to produce a mixture of tagged bound and unbound antigens or antibodies, separating said bound from said unbound, and applying said bound or said unbound to a negative ion mass spectrometer for counting the number of ions of tagging material whereby the amount of antigens or antibodies in said sample are determined.
Claim 3
Independent Claim
148 words
The method of making an immunological assay of a sample containing either an unknown amount of antigens or of a sample containing an unknown amount of antibodies, comprising: preparing a solution containing a known amount of antibodies or antigens tagged with a stable isotope or a long lived radioisotope, mixing said solution of a known amount of tagged antibodies with said unknown antigen sample or mixing said sample of said known amount of tagged antigens with said unknown antibody sample, to produce either a solution having bound and unbound tagged antibodies, or a solution having bound and unbound antigens, separating said bound from said unbound in each of said solutions, and applying an aliquot of said separated bound or unbound to a negative ion mass spectrometer for isolating and counting the number of tagging material ions to indicate the amount of antigens or antibodies present in the sample.
Claim 4
Independent Claim
148 words
The method of making an immunological assay of a sample containing either an unknown amount of antigens or of a sample containing an unknown amount of antibodies, comprising: preparing a solution containing a known amount of antibodies or antigens tagged with a stable isotope or a long lived radioisotope, mixing said solution of a known amount of tagged antibodies with said unknown antigen sample or mixing said sample of said known amount of tagged antigens with said unknown antibody sample, to produce either a solution having bound and unbound tagged antibodies, or a solution having bound and unbound antigens, separating said bound from said unbound in each of said solutions, and applying an aliquot of said separated bound or unbound to a negative ion mass spectrometer for isolating and counting the number of tagging material ions to indicate the amount of antigens or antibodies present in the sample.
Claim 5
Dependent Claim
29 words
The method as recited in claim 2 wherein said tagging material is one of a group consisting of 129 I, 36 Cl, 79 Se, 133 Te, and 14 C.
Claim 6
Dependent Claim
64 words
The method as recited in claim 2 wherein the step of counting the tagged antigens or antibodies includes: vaporizing an aliquot of sid bound or unbound tagged antibodies or antigens, ionizing said vaporized aliquot to form negative ions of the material with which said antigens or antibodies were tagged, separated said negative ions from the remainder, and counting the number of said negative ions.
Claim 7
Independent Claim
166 words
The method of making an immunological assay of a solution containing an unknown amount of antigens or a solution contining an unknown amount of antibodies, comprising: preparing a solution of a known amount of antibodies or antigens tagged with a stable isotope or a long lived radioisotope, mixing said solution of a known amount of tagged antibodies with said unknown antigen solution or said known amount of tagged antigens with said unknown antibody solution to produce either a solution having bound and unbound tagged antibodies, or a solution having bound and unbound antigens, in each of said solution separating said unbound from said bound, vaporizing an aliquot of one of said bound or unbound for each of said solution, ionizing said vaporizing aliquot for each of said solutions, separating the ions of said stable isotope from all others in said ionized vaporized aliquot, and counting said number of ions of said tagging material from which count the unknown amount of antigens or antibodies may be derived.
Claim 8
Independent Claim
166 words
The method of making an immunological assay of a solution containing an unknown amount of antigens or a solution contining an unknown amount of antibodies, comprising: preparing a solution of a known amount of antibodies or antigens tagged with a stable isotope or a long lived radioisotope, mixing said solution of a known amount of tagged antibodies with said unknown antigen solution or said known amount of tagged antigens with said unknown antibody solution to produce either a solution having bound and unbound tagged antibodies, or a solution having bound and unbound antigens, in each of said solution separating said unbound from said bound, vaporizing an aliquot of one of said bound or unbound for each of said solution, ionizing said vaporizing aliquot for each of said solutions, separating the ions of said stable isotope from all others in said ionized vaporized aliquot, and counting said number of ions of said tagging material from which count the unknown amount of antigens or antibodies may be derived.
Claim 9
Dependent Claim
31 words
The method as recited in claim 2 wherein said stable isotope of one of a group consisting of 129 I, 36 Cl, 79 Se, 133 Te, 14 C, and 3 H.
References Cited (Backward Citations)
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