Patent US4014744A successfully refreshed from Google Patents.
US4014744A
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Processes for measuring tri-, di- and monoglycerides
Assignee: Bayer Corp
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Filing: 1975-01-30
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Granted: 1977-03-29
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Expires: 1995-01-30
Abstract
Abstract In processes for measuring glycerides in a fluid, which processes require initial conversion thereof to glycerol, this conversion is achieved according to an improved and simplified saponifying reaction in which a reaction temperature between 35° and 40° C. is used.
Inventors
- 👤 Eppie Sheng Chang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 20:43
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/61; C12Q1/48; Y; Y10; Y10S; Y10S435/00; Y10S435/808
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
58 words
In a process for measuring glycerides in a fluid in which substantially all of the glycerides are saponified to glycerol with an ethanol-potassium hydroxide saponifying reagent, the improvement which consists of effecting saponification by incubating a combination of the fluid and the saponifying reagent at a temperature between 35 and 40° C. until said saponification is substantially complete.
Claim 2
Dependent Claim
58 words
In a process for measuring glycerides in a fluid in which substantially all of the glycerides are saponified to glycerol with an ethanol-potassium hydroxide saponifying reagent, the improvement which consists of effecting saponification by incubating a combination of the fluid and the saponifying reagent at a temperature between 35 and 40° C. until said saponification is substantially complete.
Claim 3
Dependent Claim
17 words
A process according to claim 1 in which said mixture is incubated for at least 12 minutes.
Claim 4
Dependent Claim
12 words
A process according to claim 1 in which said fluid is serum.
Claim 5
Dependent Claim
12 words
A process according to claim 1 in which said fluid is plasma.
Claim 6
Dependent Claim
32 words
A process according to claim 1 including as subsequent steps mixing a filtering agent and a metal salt with the saponified mixture and filtering the resulting combination to form a clear filtrate.
Claim 7
Dependent Claim
15 words
A process according to claim 5 in which said metal salt is MgSO 4 .
Claim 8
Dependent Claim
68 words
A process according to claim 6 including as subsequent steps mixing said filtrate with GK and ATP to form GP and ADP, mixing said GP and ADP with PEP and PK to form ATP and pyruvate, adding DNPH to said pyruvate and ATP to form a mixture including P-DNPH, adding a base to said mixture to obtain a basic pH and measuring the absorbance of the final mixture.
Claim 9
Dependent Claim
18 words
A process according to claim 7 in which the absorbance is measured colorimetrically between 530 and 550 nm.
References Cited (Backward Citations)
US3703591A
Cited
US3862009A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-28 | 1980-09-27 | 1981-03-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-28 | 1984-09-27 | 1985-03-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-28 | 1988-09-27 | 1989-03-29 | $7700.00 | Window Closed / Lapsed |