US4013416A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic means for the detection of protein in body fluids
Assignee: Roche Diagnostics GmbH
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Filing: 1976-02-26
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Granted: 1977-03-22
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Expires: 1996-02-26
Abstract
Abstract Protein in body fluids, e.g., urine, is detected by contacting a sample of the body fluid with a diagnostic agent comprising an absorbent carrier impregnated with a pH indicator of the octahalosulfophthalein group, a buffer, and at least one linear or branched chain polypropylene glycol immiscible with water; the resulting test papers are highly sensitive to protein without being subject to interfering effects by nitrogen-containing compounds.
Inventors
- 👤 Walter Rittersdorf
- 👤 Werner Guthlein
- 👤 Wolfgang Werner
- 👤 Hanz-Georg Rey
- 👤 Peter Rieckmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 12:41
CPC Classifications
C; C09; C09B; C09B11/00; C09B11/04; C09B11/06; C09B11/08; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6827; G01N33/6839
Claims Analysis
Total Claims: 45
Claim 1
Independent Claim
49 words
A diagnostic means for the detection of protein in body fluids, comprising an absorbent carrier impregnated with a pH indicator of the octahalosulfophthalein group, a buffer, and at least one linear or branched chain polypropylene glycol immiscible with water and having a molecular weight of from 500 to 10,000.
Claim 2
Dependent Claim
49 words
A diagnostic means for the detection of protein in body fluids, comprising an absorbent carrier impregnated with a pH indicator of the octahalosulfophthalein group, a buffer, and at least one linear or branched chain polypropylene glycol immiscible with water and having a molecular weight of from 500 to 10,000.
Claim 3
Dependent Claim
16 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol contains other lower oxyalkylene groups.
Claim 4
Dependent Claim
20 words
Diagnostic means as claimed in claim 1 wherein the polypropylene glycol has a molecular weight of from 1,200 to 6,300.
Claim 5
Dependent Claim
14 words
Diagnostic means as claimed in claim 1 wherein the absorbent carrier is filter paper.
Claim 6
Dependent Claim
14 words
Diagnostic means as claimed in claim 1 wherein the absorbent carrier is fiber fleece.
Claim 7
Dependent Claim
13 words
Diagnostic means as claimed in claim 1 wherein the absorbent carrier is asbestos.
Claim 8
Dependent Claim
23 words
Diagnostic means as claimed in claim 1 wherein the buffer is citric acid in admixture with an alkaline metal or ammonium salt thereof.
Claim 9
Dependent Claim
23 words
Diagnostic means as claimed in claim 1 wherein the buffer is malic acid in admixture with an alkali metal or ammonium salt thereof.
Claim 10
Dependent Claim
23 words
Diagnostic means as claimed in claim 1 wherein the buffer is tartaric acid in admixture with an alkali metal or ammonium salt thereof.
Claim 11
Dependent Claim
12 words
Diagnostic means as claimed in claim 1 also containing a wetting agent.
Claim 12
Dependent Claim
12 words
Diagnostic means as claimed in claim 1 also containing a complex-forming agent.
Claim 13
Dependent Claim
14 words
Diagnostic means as claimed in claim 1 also containing a swelling and/or thickening agent.
Claim 14
Dependent Claim
21 words
Diagnostic means as claimed in claim 1 substantially comprising on said absorbent carrier, ammonium citrate buffer, 3',3",5',5"-tetrachlorophenol-3,4,5,6-tetrabromosulfophthalein, and said polypropylene glycol.
Claim 15
Dependent Claim
21 words
Diagnostic means as claimed in claim 1 substantially comprising on said absorbent carrier, ammonium citrate buffer, 3',3",5',5",3,4,5,6-octabromophenolsulfophthalein, and said polypropylene glycol.
Claim 16
Dependent Claim
21 words
Diagnostic means as claimed in claim 1 substantially comprising on said absorbent carrier, sodium malate buffer 3',3",5',5",3,4,5,6-octabromophenolsulfophthalein, and said polypropylene glycol.
Claim 17
Dependent Claim
21 words
Diagnostic means as claimed in claim 1 substantially comprising on said absorbent carrier, sodium citrate buffer 3',3",5',5",3,4,5,6-octachlorophenolsulfophthalein, and said polypropylene glycol
Claim 18
Dependent Claim
21 words
Diagnostic means as claimed in claim 1 substantially comprising on said absorbent carrier, sodium citrate buffer, 3',3"-dibromo-5',5"-dichlorophenol-3,4,5,6-tetrachlorosulfophthalein, and said polypropylene glycol.
Claim 19
Dependent Claim
21 words
Diagnostic means as claimed in claim 1 substantially comprising on said absorbent carrier, sodium citrate buffer, 3',3",5',5"-tetrabromophenol-3,4,5,6-tetrachlorosulfophthalein, and said polypropylene glycol.
Claim 20
Dependent Claim
24 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a linear polypropylene glycol with an average molecular weight of about 4,000.
Claim 21
Dependent Claim
24 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a linear polypropylene glycol with an average molecular weight of about 2,000.
Claim 22
Dependent Claim
24 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a linear polypropylene glycol with an average molecular weight of about 1,200.
Claim 23
Dependent Claim
36 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a branched polypropylene glycol modified with ethylene oxide and has an average molecular weight of about 3,800 and an hydroxyl number of about 42.
Claim 24
Dependent Claim
36 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a branched polypropylene glycol modified with ethylene oxide and has an average molecular weight of about 3100 and a hydroxyl number of about 49.
Claim 25
Dependent Claim
33 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a partially branched polypropylene glycol and has an average molecular weight of about 3500 and a hydroxyl number of about 46.
Claim 26
Dependent Claim
36 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a branched polypropylene glycol modified with ethylene oxide and has an average molecular weight of about 3000 and a hydroxyl number of about 56.
Claim 27
Dependent Claim
39 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a branched polypropylene glycol based on trimethylolpropane modified with ethylene oxide and has an average molecular weight of about 6300 and a hydroxyl number of about 27.
Claim 28
Dependent Claim
35 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a branched polypropylene glycol based on trimethylolpropane and has an average molecular weight of about 2600 and a hydroxyl number of about 64.
Claim 29
Dependent Claim
35 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a branched polypropylene glycol based on glycerol and has an average molecular weight of about 3800 and a hydroxyl number of about 29.
Claim 30
Dependent Claim
34 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a branched polypropylene glycol based on trimethylolpropane and has a molecular weight of about 4500 and a hydroxyl number of about 37.
Claim 31
Dependent Claim
38 words
Diagnostic means as claimed in claim 1 wherein said polypropylene glycol is a linear polypropylene glycol modified with ethylene oxide in an amount up to 10% of the glycol and has an average molecular weight of about 3800.
Claim 32
Dependent Claim
29 words
Process for the preparation of a diagnostic means as claimed in claim 1 wherein said absorbent carrier is impregnated with a solution of the indicator, buffer and propylene glycol.
Claim 33
Dependent Claim
40 words
Process for the preparation of a diagnostic means as claimed in claim 1 wherein an absorbent carrier is first impregnated with an aqueous solution of the buffer and thereafter with a solution of the other components in an organic solvent.
Claim 34
Dependent Claim
44 words
Process as claimed in claim 31 wherein 100 ml of impregnation solution contains from about 0.5 to 5 g. of polypropylene glycol, from 10 to 30 g. of buffer, from 0.02 to 0.2 g. of indicator and 0.0 to 1.0 g. of surface-active adjuvant.
Claim 35
Dependent Claim
58 words
Process as claimed in claim 32 wherein 100 ml of the aqeuous solution contains from 10 to 30 g. of buffer and 100ml of the solution of the other components in an organic solvent contains from about 0.5 to 5g. of polypropylene glycol, from 0.02 to 0.2 g. of indicator and 0.0 to 1.0 g. of surface-active adjuvant.
Claim 36
Dependent Claim
22 words
Process as claimed in claim 31 wherein 100 ml. of the impregnation solution contains from 1 to 2 g. of polypropylene glycol.
Claim 37
Dependent Claim
21 words
Process as claimed in claim 31 wherein 100 ml. of the impregnation solution contains from 15 to 20 g. of buffer.
Claim 38
Dependent Claim
21 words
Process as claimed in claim 31 wherein 100 ml. of the impregnation solution contains from 0.05 to 0.1 g. of indicator.
Claim 39
Dependent Claim
21 words
Process as claimed in claim 31 wherein 100 ml. of impregnation solution contains from 0.2 to 0.5 g. of surface-active adjuvant.
Claim 40
Dependent Claim
24 words
Process as claimed in claim 32 wherein 100 ml. of the organic solvent impregnation solution contains from 1 to 2 g. of polypropylene glycol.
Claim 41
Dependent Claim
23 words
Process as claimed in claim 32 wherein 100 ml of the aqueous buffer impregnation solution contains from 15 to 20 g. of buffer.
Claim 42
Dependent Claim
23 words
Process as claimed in claim 32 wherein 100 ml. of the organic solvent impregnation solution contains from 0.5 to 0.1 g. of indicator.
Claim 43
Dependent Claim
24 words
Process as claimed in claim 32 wherein 100 ml. of the organic solvent impregnation solution contains from 0.2 to 0.5 g. of surface-active adjuvant.
Claim 44
Dependent Claim
22 words
Diagnostic means as claimed in claim 1 wherein said impregnated absorbent carrier is sealed between synthetic resin film and a fine-mesh material.
Claim 45
Dependent Claim
18 words
Diagnostic means as claimed in claim 1 wherein said impregnated absorbent carrier is adhered to a plastic strip.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-21 | 1980-09-20 | 1981-03-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-21 | 1984-09-20 | 1985-03-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-21 | 1988-09-20 | 1989-03-22 | $7700.00 | Window Closed / Lapsed |