US3873272A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reagent and method for albumin determination
Assignee: Baxter International Inc
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Filing: 1974-02-20
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Granted: 1975-03-25
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Expires: 1994-02-20
Abstract
Abstract A reagent for albumin determination comprising an aqueous solution of 0.1 - 0.2 grams of bromcresol green, 4 - 6 grams of sodium salt of EDTA and 4.5 - 6 ml. of polyoxyethylene fatty alcohol ether per liter of said solution buffered to pH 4 - 4.2.
Inventors
- 👤 Elisabeth D Wakefield
- 👤 Patricia A Friedman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 05:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6827; G01N33/6839
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
113 words
A reagent for use in the determination of albumin consisting essentially of an aqueous solution of from about 0.1 to about 0.2 gram of bromcresol green, from about 4 to about 6 grams of sequestering agent selected from the group consisting of disodium and tetrasodium salts of ethylenediamine tetraacetic acid, from about 4.5 to about 6 ml. of nonionic surfactant selected from the group consisting of polyoxyethylene fatty alcohol ethers having from about 12 to about 30 carbon atoms in the alcohol moiety and from about 2 to about 23 ethylene oxides in the molecule, per liter of said aqueous solution and buffered to a pH of from about 4 to about 4.2.
Claim 2
Dependent Claim
113 words
A reagent for use in the determination of albumin consisting essentially of an aqueous solution of from about 0.1 to about 0.2 gram of bromcresol green, from about 4 to about 6 grams of sequestering agent selected from the group consisting of disodium and tetrasodium salts of ethylenediamine tetraacetic acid, from about 4.5 to about 6 ml. of nonionic surfactant selected from the group consisting of polyoxyethylene fatty alcohol ethers having from about 12 to about 30 carbon atoms in the alcohol moiety and from about 2 to about 23 ethylene oxides in the molecule, per liter of said aqueous solution and buffered to a pH of from about 4 to about 4.2.
Claim 3
Dependent Claim
23 words
The reagent of claim 1 in which the sequestering agent is disodium ethylenediamine tetraacetate and the nonionic surfactant is polyoxyethylene (23) lauryl ether.
Claim 4
Dependent Claim
106 words
IN THE METHOD OF DETERMINING SERUM ALBUMIN BY THE USE OF BROMCRESOL GREEN, TOGETHER WITH SEQUESTERING AGENT IN AQUEOUS SOLUTION BUFFERED TO A PH OF 4-4.2, THE IMPROVEMENT COMPRISING EMPLOYING FROM ABOUT 4 TO ABOUT 6 GRAMS OF SEQUESTERING AGENT SELECTED FROM THE GROUP CONSISTING OF DISODIUM AND TETRASODIUM SALTS OF ETHYLENEDIAMINE TETRAACETIC ACID, AND FROM ABOUT 4.5 TO ABOUT 6 ML. OF NONIONIC SURFACTANT SELECTED FROM THE GROUP CONSISTING OF POLYOXYETHYLENE FATTY ALCOHOL ETHERS HAVING FROM ABOUT 12 TO ABOUT 30 CARBON ATOMS IN THE ALCOHOL MOIETY AND FROM ABOUT 2 TO ABOUT 23 ETHYLENE OXIDES IN THE MOLECULE, PER LITER OF SAID AQUEOUS SOLUTION.
Claim 5
Dependent Claim
106 words
IN THE METHOD OF DETERMINING SERUM ALBUMIN BY THE USE OF BROMCRESOL GREEN, TOGETHER WITH SEQUESTERING AGENT IN AQUEOUS SOLUTION BUFFERED TO A PH OF 4-4.2, THE IMPROVEMENT COMPRISING EMPLOYING FROM ABOUT 4 TO ABOUT 6 GRAMS OF SEQUESTERING AGENT SELECTED FROM THE GROUP CONSISTING OF DISODIUM AND TETRASODIUM SALTS OF ETHYLENEDIAMINE TETRAACETIC ACID, AND FROM ABOUT 4.5 TO ABOUT 6 ML. OF NONIONIC SURFACTANT SELECTED FROM THE GROUP CONSISTING OF POLYOXYETHYLENE FATTY ALCOHOL ETHERS HAVING FROM ABOUT 12 TO ABOUT 30 CARBON ATOMS IN THE ALCOHOL MOIETY AND FROM ABOUT 2 TO ABOUT 23 ETHYLENE OXIDES IN THE MOLECULE, PER LITER OF SAID AQUEOUS SOLUTION.
Claim 6
Dependent Claim
23 words
The method of claim 3 in which the sequestering agent is disodium ethylenediamine tetraacetate and the nonionic surfactant is polyoxyethylene (23) lauryl ether.
References Cited (Backward Citations)
US3533749A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-24 | 1978-09-23 | 1979-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-24 | 1982-09-23 | 1983-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-24 | 1986-09-23 | 1987-03-25 | $7700.00 | Window Closed / Lapsed |