Patent US3989594A successfully refreshed from Google Patents.
US3989594A
Expired / Lapsed
US
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Microbiological production of protein
Assignee: Imperial Chemical Industries Ltd
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Filing: 1973-11-15
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Granted: 1976-11-02
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Expires: 1993-11-15
Abstract
Abstract Growth of bacteria belonging to new species of genera Pseudomonas, Microcyclus and Hyphomicrobium upon culture media comprising sources of assimilable carbon, for example oxygenated hydrocarbons, and inorganic nutrients to produce protein compositions and/or other fermentation products, for example amino acids. The protein compositions produced are suitable for use as protein supplements in human and animal foods.
Inventors
- 👤 David Graham MacLennan
- 👤 John Clark Ousby
- 👤 Terence Robert Owen
- 👤 David Charles Steer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 06:07
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/32; Y; Y10; Y10S; Y10S435/00; Y10S435/804; Y10S435/8215; Y10S435/822; Y10S435/874
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
68 words
A process for the microbiological production of a protein composition as the essential product which comprises aerobically culturing bacteria belonging to at least one methanol-utilizing strain of a species selected from the group consisting of Pseudomonas methylotropha, and Pseudomonas rosea, in an aqueous culture medium comprising methanol as a source of assimilable carbon and inorganic nutrients to produce said protein composition and recovering the protein composition so produced.
Claim 2
Dependent Claim
68 words
A process for the microbiological production of a protein composition as the essential product which comprises aerobically culturing bacteria belonging to at least one methanol-utilizing strain of a species selected from the group consisting of Pseudomonas methylotropha, and Pseudomonas rosea, in an aqueous culture medium comprising methanol as a source of assimilable carbon and inorganic nutrients to produce said protein composition and recovering the protein composition so produced.
Claim 3
Dependent Claim
29 words
A process according to claim 1 wherein the bacteria belong to at least one strain selected from the group comprising NCIB Nos. 10508 to 10515 and 10592 to 10612.
Claim 4
Dependent Claim
38 words
A process according to claim 1 wherein the culture medium comprises a nitrogen source, selected from the group consisting of ammonia, an ammonium salt, urea and a nitrate, and inorganic sources of phosphorus, sulphur, magnesium, potassium and sodium.
Claim 5
Dependent Claim
27 words
A process according to claim 1 wherein the culture medium comprises ions of an element selected from the group consisting of calcium, copper, iron, cobalt and manganese.
Claim 6
Dependent Claim
86 words
A process according to claim 3 wherein the culture medium contains the carbon source in proportions between 0.05% and 10% by weight, the nitrogen source in proportions between 0.001% and 3% by weight of elementary nitrogen and the sources of phosphorus, sulphur, magnesium, potassium and sodium in amounts sufficient to provide the weight percent ion concentrations PO 4 3 - 0.01 to 0.5; SO 4 2 - 0.01 to 0.25; Mg 2 + 0.001 to 0.1; K + 0.01 to 0.25; Na + 0.002 to 0.06.
Claim 7
Dependent Claim
20 words
A process according to claim 1 wherein culturing is effected at a temperature within the range 20° to 50° C.
Claim 8
Dependent Claim
20 words
A process according to claim 6 wherein culturing is effected at a temperature within the range 34° to 45° C.
Claim 9
Dependent Claim
12 words
A process according to claim 3 wherein the species is Pseudomonas methylotropha.
Claim 10
Dependent Claim
12 words
A process according to claim 3 wherein the species is Pseudomonas rosea.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-11-02 | 1980-05-03 | 1980-11-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-11-02 | 1984-05-03 | 1984-11-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-11-02 | 1988-05-03 | 1988-11-02 | $7700.00 | Window Closed / Lapsed |