Patent US3983002A successfully refreshed from Google Patents.
US3983002A
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Process for preparation of cellulase
Assignee: Amano Enzyme Inc
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Filing: 1974-11-08
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Granted: 1976-09-28
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Expires: 1994-11-08
Abstract
Abstract Aeromonas sp. No. 212 is cultured to allow it to produce a novel cellulase 212 and/or novel hemicellulase in a culture medium, and the so produced cellulase 212 and/or hemicellulase 333 is separated from the culture medium. The so separated novel cellulase 212 and/or novel hemicellulase 333 is used as a cellulosic material-decomposing agent in purifying tanks, plants for treatment of industrial waste waters, excrement treatment plants, drainage disposal treatment plants and the like.
Inventors
- 👤 Takaichi Ohya
- 👤 Nobumasa Yokoi
- 👤 Tamio Mase
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 15:02
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; A; A23; A23F; A23F5/00; A23F5/16; A23F5/163; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C11D3/38645; C12N9/2477; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
67 words
A process for preparing cellulase 212 comprising: aerobically culturing Aeromonas sp. No. 212, FERM-P No. 2306, ATCC No. 31085, in a culture medium containing a nitrogen source, a carbon source and a nutrient while maintaining the pH of the culture medium at 6-10 until cellulase is produced and accumulated therein; and separating and recovering the cellulase produced and accumulated in the culture medium during the culturing step.
Claim 2
Dependent Claim
67 words
A process for preparing cellulase 212 comprising: aerobically culturing Aeromonas sp. No. 212, FERM-P No. 2306, ATCC No. 31085, in a culture medium containing a nitrogen source, a carbon source and a nutrient while maintaining the pH of the culture medium at 6-10 until cellulase is produced and accumulated therein; and separating and recovering the cellulase produced and accumulated in the culture medium during the culturing step.
Claim 3
Dependent Claim
21 words
A process in accordance with claim 1, wherein the pH is adjusted to 7-9 at the initial stage of the culturing.
Claim 4
Dependent Claim
18 words
A process in accordance with claim 1 wherein, said culturing step is conducted at 30°-45°C. for 2-5 days.
Claim 5
Dependent Claim
66 words
A process for preparing hemicellulase 333 comprising: aerobically culturing Aeromonas sp. No. 212, FERM-P No. 2306, ATCC No. 31085, in culture medium containing a nitrogen source, a carbon source and a nutrient while maintaining the pH of the culture medium at 6-10 until hemicellulase is produced and accumulated therein; and separating and recovering the hemicellulase produced and accumulated in the culture medium during the culturing step.
Claim 6
Dependent Claim
66 words
A process for preparing hemicellulase 333 comprising: aerobically culturing Aeromonas sp. No. 212, FERM-P No. 2306, ATCC No. 31085, in culture medium containing a nitrogen source, a carbon source and a nutrient while maintaining the pH of the culture medium at 6-10 until hemicellulase is produced and accumulated therein; and separating and recovering the hemicellulase produced and accumulated in the culture medium during the culturing step.
Claim 7
Dependent Claim
18 words
A process in accordance with claim 4, wherein said culturing step is conducted at 30°-45°C. for 2-5 days.
Claim 8
Dependent Claim
74 words
A process for preparing both cellulase 212 and hemicellulase 333, comprising: aerobically culturing Aeromonas sp. No. 212, FERM-P No. 2306, ATCC No. 31085, in a culture medium containing a nitrogen source, a carbon source and a nutrient while maintaining the pH of the culture medium at 6-10 until both cellulase and hemicellulase are produced and accumulated therein; and recovering the cellulase and hemicellulase produced and accumulated in the culture medium during the culturing step.
Claim 9
Dependent Claim
74 words
A process for preparing both cellulase 212 and hemicellulase 333, comprising: aerobically culturing Aeromonas sp. No. 212, FERM-P No. 2306, ATCC No. 31085, in a culture medium containing a nitrogen source, a carbon source and a nutrient while maintaining the pH of the culture medium at 6-10 until both cellulase and hemicellulase are produced and accumulated therein; and recovering the cellulase and hemicellulase produced and accumulated in the culture medium during the culturing step.
References Cited (Backward Citations)
US3844890A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-09-28 | 1980-03-29 | 1980-09-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-09-28 | 1984-03-29 | 1984-09-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-09-28 | 1988-03-29 | 1988-09-28 | $7700.00 | Window Closed / Lapsed |