US3844890A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Alkaline cellulase and preparation of the same
Assignee: RIKEN
•
Filing: 1972-09-27
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Granted: 1974-10-29
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Expires: 1992-09-27
Abstract
Abstract A novel alkaline cellulase characterized by being an enzyme having an optimal pH of about from 8 to 10 at a temperature of 40* C and a method for preparing thereof characterized by fermentation of a strain of microorganism selected from the group consisting of Bacillus N1 and Bacillus N4 in an alkaline culture medium containing a carbonate.
Inventors
- 👤 M Nakao
- 👤 K Horikoshi
- 👤 Y Ikeda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 09:48
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12Y; C12Y302/00; C12Y302/01; C12Y302/01004; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/838
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
45 words
AN ALKALINE CELLULASE WHICH IS A MEMBER OF THE GROUP OF THE ALKALINE CELLUS LASE FROM BACILLUS N1(ATCC 21832) AND THE ALKALINE CELLULASE FROM BACILLUS N4 (ATCC 21833) SAID ALKALINE CELLULASE HAVING OPTIMUM STABILITY IN THE PH RANGE OF 810 AT A TEMPERATURE OF 40*C.
Claim 2
Dependent Claim
45 words
AN ALKALINE CELLULASE WHICH IS A MEMBER OF THE GROUP OF THE ALKALINE CELLUS LASE FROM BACILLUS N1(ATCC 21832) AND THE ALKALINE CELLULASE FROM BACILLUS N4 (ATCC 21833) SAID ALKALINE CELLULASE HAVING OPTIMUM STABILITY IN THE PH RANGE OF 810 AT A TEMPERATURE OF 40*C.
Claim 3
Dependent Claim
110 words
A process for preparing the novel alkaline cellulase having physicochemical properties defined in claim 1, comprising inoculating a strain of a microorganism selected from the group consisting of Bacillus N1 (ATCC 21832) and Bacillus N4 (ATCC 21833) in a culture medium composed of an alkali carbonate, a carbon source, a nitrogen source, and inorganic material, cultivating the said culture medium at pH 8 - 11, at temperatures of about 20* to 40* C for a period sufficient to impart substantial enzyme activity in the said culture medium and to produce the said alkaline cellulase in the said culture medium, and collecting the said alkaline cellulase from the said culture medium.
Claim 4
Dependent Claim
18 words
A process according to claim 2, wherein the culture is carried out under aerobic submerged condition with stirring.
Claim 5
Dependent Claim
18 words
A process according to claim 2, wherein the culture is carried out for about 24 to 75 hours.
Claim 6
Dependent Claim
21 words
The alkaline cellulase from the microorganism Bacillus N4, according to claim 1, which when pure exhibits maximum activity at pH 10.
Claim 7
Dependent Claim
19 words
The enzyme according to claim 1 which is not deactivated by ethylene diamine tetraacetate or by p-chloromercury benzoic acid.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-10-28 | 1978-04-29 | 1978-10-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-10-28 | 1982-04-29 | 1982-10-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-10-28 | 1986-04-29 | 1986-10-29 | $7700.00 | Window Closed / Lapsed |