US3977942A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fermentation of cephamycin C
Assignee: Merck and Co Inc
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Filing: 1975-11-21
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Granted: 1976-08-31
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Expires: 1995-11-21
Abstract
Abstract Increased yields of the known and useful antibiotic cephamycin C [7-(D-5-amino-5-carboxyvaleramido)-3-(carbamoyloxymethyl)-7-methoxy-3- cephem-4-carboxylic acid] are obtained by the addition of D- or DL-arginine, D- or DL-ornithine and/or one of several polyamines; or D- or DL-lysine in combination with one of several polyamines to fermentation media composed of complex organic nutrients.
Inventors
- 👤 Edward Inamine
- 👤 Jerome Birnbaum
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 06:23
CPC Classifications
C; C12; C12P; C12P35/00; C12P35/08; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
60 words
In the process for preparing cephamycin C by growing a cephamycin C producing species of Streptomyces in a nutrient medium, the improvement which comprises the addition of the amino acid D- or DL-arginine, D- or DL-ornithine or one of several polyamines selected from the group consisting of 1,3-diaminopropane; 1,3-diamino-2-hydroxypropane; N-methyl-1,3-diaminopropane, agmatine, spermidine, spermine, cadaverine and putrescine to the nutrient medium.
Claim 2
Dependent Claim
60 words
In the process for preparing cephamycin C by growing a cephamycin C producing species of Streptomyces in a nutrient medium, the improvement which comprises the addition of the amino acid D- or DL-arginine, D- or DL-ornithine or one of several polyamines selected from the group consisting of 1,3-diaminopropane; 1,3-diamino-2-hydroxypropane; N-methyl-1,3-diaminopropane, agmatine, spermidine, spermine, cadaverine and putrescine to the nutrient medium.
Claim 3
Dependent Claim
12 words
The process of claim 1 wherein the Streptomyces specie is Streptomyces lactamdurans.
Claim 4
Dependent Claim
15 words
The process of claim 2 wherein the nutrient medium is a complex organic nutrient medium.
Claim 5
Dependent Claim
109 words
The process of claim 3 wherein the nutrient medium contains from about 0.025 to about 0.40 percent (weight/volume) of the amino acids D- or DL-arginine or D- or DL-ornithine calculated as the hydrochloride; or from about 0.01 to about 0.2% (weight/volume) of 1,3-diaminopropane calculated as the dihydrochloride; or from about 0.0025 to 0.05% (weight/volume) of spermidine or spermine calculated as the trihydrochloride and tetrahydrochloride respectively; or from about 0.025 to 0.2% (weight/volume) of cadaverine or putrescine calculated as the dihydrochloride; or from about 0.025 to 0.2% (weight/volume) of agmatine calculated as the sulfate; or from about 0.05 to about 0.2% (weight/volume) of 1,3-diamino-2-hydroxypropane or N-methyl-1,3-diaminopropane as the free base.
Claim 6
Dependent Claim
60 words
In the process for preparing cephamycin C by growing a cephamycin C producing species of Streptomyces in a nutrient medium, the improvement which comprises the addition of the amino acid D- or DL-arginine, D- or DL-ornithine or D- or DL-lysine in combination with a polyamine selected from the group consisting of 1,3-diaminopropane; 1,3-diamino-2-hydroxypropane; N-methyl-1,3-diaminopropane, agmatine, spermidine, spermine, cadaverine and putrescine.
Claim 7
Dependent Claim
60 words
In the process for preparing cephamycin C by growing a cephamycin C producing species of Streptomyces in a nutrient medium, the improvement which comprises the addition of the amino acid D- or DL-arginine, D- or DL-ornithine or D- or DL-lysine in combination with a polyamine selected from the group consisting of 1,3-diaminopropane; 1,3-diamino-2-hydroxypropane; N-methyl-1,3-diaminopropane, agmatine, spermidine, spermine, cadaverine and putrescine.
Claim 8
Dependent Claim
12 words
The process of claim 5 wherein the Streptomyces species is Streptomyces lactamdurans.
Claim 9
Dependent Claim
15 words
The process of claim 6 wherein the nutrient medium is a complex organic nutrient medium.
Claim 10
Dependent Claim
92 words
The process of claim 7 wherein the nutrient medium contains the amino acids D- or DL-arginine, D- or DL-ornithine or D- or DL-lysine to the extent of about 0.1 to about 0.2% (weight/volume) calculated as the hydrochloride in combination with about 0.1% (weight/volume) of 1,3-diaminopropane or cadaverine or putrescine calculated as the dihydrochloride; or about 0.02% (weight/volume) of spermidine or spermine calculated as the trihydrochloride and tetrahydrochloride respectively; or about 0.1% (weight/volume) agmatine calculated as the sulfate; or about 0.05 to about 0.2% (weight/volume) of 1,3-diamino-2-hydroxypropane or N-methyl-1,3-diaminopropane as the free base.
Claim 11
Dependent Claim
26 words
The process according to claim 8 wherein DL-lysine and 1,3-diaminopropane is added to the extent of about 0.1% (weight/volume) calculated as the hydrochloride and dihydrochloride respectively.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-08-31 | 1980-03-01 | 1980-08-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-08-31 | 1984-03-01 | 1984-08-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-08-31 | 1988-03-01 | 1988-08-31 | $7700.00 | Window Closed / Lapsed |