US3769169A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fermentation process
Assignee: Merck and Co Inc
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Filing: 1971-05-20
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Granted: 1973-10-30
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Expires: 1991-05-20
Abstract
Abstract A method for improving the yield of 7-(D-5-amino-5carboxylvaleramido)-3-(carbamoyloxymethyl)-7-methoxy-3-cephem -4carboxylic acid by adding to fermentation media an additive selected from glycine, L-phenylalanine, a carbamate or an amide, or combinations thereof. The product is a new antibiotic produced from a new strain of Streptomyces; it exhibits activity against gram-negative and gram-positive bacteria.
Inventors
- 👤 J Birnbaum
- 👤 E Inamine
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 08:32
CPC Classifications
C; C12; C12P; C12P35/00; C12P35/08; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
49 words
The method of claim 1 wherein the pH of the aqueous nutrient medium is in the range of from about 6.0 to 8.0 and contains between about 1 percent and 6 percent by weight of carbohydrate and between about 0.2 percent and 6 percent by weight of available nitrogen.
Claim 2
Dependent Claim
14 words
The method of claim 1 wherein the carbamate is a compound of the formula:
Claim 3
Dependent Claim
14 words
The method of claim 3 wherein the amide is formamide or N,N-di-lower alkyl formamide.
Claim 4
Dependent Claim
20 words
The method of claim 3 wherein the amide employed is N,N-diisobutylpropionamide at a concentration of from about 0.063 -0.125 percent.
Claim 5
Dependent Claim
21 words
The method of claim 3 wherein the carbamate employed is N-2-hydroxyethylcarbamate at a concentration of from about 0.2 - 0.8 percent.
Claim 6
Dependent Claim
22 words
The method of claim 3 wherein the carbamate or amide is employed at a concentration of from about 0.0156 - 2.0 percent.
Claim 7
Dependent Claim
10 words
The method of claim 3 wherein the amide is N,N-dimethylformamide.
Claim 8
Dependent Claim
19 words
The method of claim 1 wherein glycine is employed at a concentration of from about 0.01 - 0.10 percent.
Claim 9
Dependent Claim
19 words
The method of claim 1 wherein L-phenylalanine is employed at a concentration of from about 0.05 - 0.3 percent.
Claim 10
Dependent Claim
50 words
In the method for preparing 7-(D-5-amino-5-carboxyvaleramido)-3-(carbamoyloxymethyl)-7-methoxy-3-cephem -4-carboxylic acid by growing Streptomyces lactamdurans in a nutrient medium, the improvement which comprises using 0.05% glycine, 0.3% L-phenylalanine, 0.2 - 0.8 percent ethyl N-2-hydroxy-ethylcarbamate or 0.063 - 0.125 percent N,N-diisobutylpropionamide as an additive to the fermentation medium to improve the yield of antibiotic.
Claim 11
Dependent Claim
18 words
The method of claim 11 wherein either N-2-hydroxyethylcarbamate or N,N-diisobutylpropionamide is used in combination with glycine and L-phenylalanine.
References Cited (Backward Citations)
US3436310A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-29 | 1977-04-30 | 1977-10-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-29 | 1981-04-30 | 1981-10-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-29 | 1985-04-30 | 1985-10-30 | $7700.00 | Window Closed / Lapsed |