Process for purified rabies vaccine

Claim 3

A method of claim 1 which consists of: A. centrifugation at from about 45,000 to about 65,000 G. for from about 20 to about 40 minutes; B. collecting and dispersing the solid phase in a physiologically-acceptable vaccine diluent to produce a suspension; C. centrifugation at from about 1,000 to about 2,000 G. for from about 4 to about 8 minutes; and D. collecting the liquid phase.

Claim 4

A purified egg-embryo-derived rabies vaccine, prepared by the following process: A. infecting embryonated duck eggs with rabies virus; B. incubating the eggs at about 36°C. for about 14 days; C. harvesting the embryos from the infected eggs; D. grinding and dispersing the embryos in a quantity of a physiologically-acceptable vaccine diluent which provides a final dispersed volume of the egg-embryo-derived crude rabies vaccine which does not exceed 1 ml. per 200 mg. of embryos dispersed therein; E. removing gross amounts of tissue by straining or centrifuging; F. purifying the crude rabies vaccine by the method of claim 1; and G. inactivating the vaccine, which inactivation may take place either before or after purifying the vaccine; each milliliter of which purified rabies vaccine represents at least 200 mg. of embryos, and which vaccine contains less than about 15 mg. of lipids per g. of embryos represented by the vaccine and also contains less than about 2 mg. of protein nitrogen per g. of embryos represented by the vaccine.