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US3965035A
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Enzyme carrier regeneration
Assignee: Corning Glass Works
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Filing: 1974-09-18
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Granted: 1976-06-22
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Expires: 1994-09-18
Abstract
Abstract Highly porous MgO-Al2O3 support materials useful for the immobilization of glucose isomerase can be regenerated for reuse by pyrolysis under conditions sufficient to remove substantially all carbonaceous matter, followed by treatment with a neutralized citrate solution.
Inventors
- 👤 Lorraine R. Bialousz
- 👤 Ethel R. Herritt
- 👤 Donald J. Lartigue
- 👤 Wayne H. Pitcher, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 15:31
CPC Classifications
B; B01; B01J; B01J21/00; B01J21/20; B01J31/00; B01J31/003; B01J31/02; B01J31/04; B01J35/00; B01J35/60; B01J35/64; B01J35/647; B01J35/651; C; C12; C12P; C12P1/00; C12P19/00; C12P19/24; B01J35/40; B01J35/45; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/584
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
126 words
A method of regenerating a porous inorganic support material used for the adsorption of glucose isomerase and comprising porous particles of a MgO-Al 2 O 3 composition wherein the amount of MgO is within the range of about 0.84 to 12.0% by weight, the particles have an average particle size within the range of about 4 to 200 mesh, U.S. Standard Sieve, and an average pore diameter within the range of about 100A to 1000A, the method comprising the steps of pyrolyzing the material at a temperature within the range of about 500°C. to 900°C. under conditions sufficient to remove essentially all carbonaceous matter and then reacting the material with an aqueous solution of citrate ions, the solution having a pH between about 6.0 and 10.0.
Claim 2
Independent Claim
126 words
A method of regenerating a porous inorganic support material used for the adsorption of glucose isomerase and comprising porous particles of a MgO-Al 2 O 3 composition wherein the amount of MgO is within the range of about 0.84 to 12.0% by weight, the particles have an average particle size within the range of about 4 to 200 mesh, U.S. Standard Sieve, and an average pore diameter within the range of about 100A to 1000A, the method comprising the steps of pyrolyzing the material at a temperature within the range of about 500°C. to 900°C. under conditions sufficient to remove essentially all carbonaceous matter and then reacting the material with an aqueous solution of citrate ions, the solution having a pH between about 6.0 and 10.0.
Claim 3
Dependent Claim
23 words
The method of claim 1 wherein the citrate solution is about a 0.1 molar sodium citrate solution, having a pH of about 7.0.
Claim 4
Dependent Claim
21 words
The method of claim 2 wherein the amount of citrate solution used is about 3 ml. for each gram of carrier.
Claim 5
Dependent Claim
31 words
The method of claim 1 wherein the treatment with the citrate solution occurs in a plugged flow-through column containing the support material and through which the citrate solution is continuously passed.
Claim 6
Dependent Claim
24 words
The method of claim 1 wherein the pyrolysis is at a temperature of between about 600°C. and 900°C. for at least about 1 hour.
Claim 7
Dependent Claim
22 words
The method of claim 5 wherein the reaction with the citrate solution occurs within a plugged flow-through column containing the support material.
Claim 8
Dependent Claim
38 words
The method of claim 1 wherein the pyrolysis is at a temperature of about 500-600°C. for about 1 hour and the citrate solution consists of a 0.1 molar sodium citrate solution neutralized to a pH of about 7.0.
Claim 9
Dependent Claim
28 words
The method of claim 7 wherein the treatment with the citrate solution is accomplished by continuously passing the solution through a plugged flow-through column containing the support material.
Claim 10
Dependent Claim
24 words
The method of claim 8 wherein the amount of citrate solution is about 3 ml. for each gram of support material in the column.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-22 | 1979-12-22 | 1980-06-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-22 | 1983-12-22 | 1984-06-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-22 | 1987-12-22 | 1988-06-22 | $7700.00 | Window Closed / Lapsed |