US3847740A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the production of levulose-bearing syrups
Assignee: Genencor International Wisconsin Inc
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Filing: 1973-09-28
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Granted: 1974-11-12
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Expires: 1993-09-28
Abstract
Abstract Levulose-bearing syrups are produced by passing a dextrose solution through a column or bed of an immobilized dextrose isomerase enzyme preparation. The useful life of the enzyme preparation is extended by conducting the isomerization initially at a low operating temperature, then increasing the temperature of operation after an initial decrease in enzyme activity has been observed. Also disclosed is an improved immobilized xylose isomerase enzyme preparation which is prepared by contacting a solution of xylose isomerase with particulate basic magnesium carbonate. The novel immobilized enzyme preparation is useful in enzymatically isomerizing dextrose solutions to levulose-bearing syrups.
Inventors
- 👤 R Heady
- 👤 W Jacaway
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 15:33
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12P; C12P19/00; C12P19/24; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
19 words
A STABILIZED XYLOSE ISOMERASE ENZYME PREPARATION COMPRISING A CELL FREE XYLOSE ISOMERASE ENZYME SORBED ON PARTICULATE BASIC MAGNESIUM CARBONATE.
Claim 2
Dependent Claim
19 words
A STABILIZED XYLOSE ISOMERASE ENZYME PREPARATION COMPRISING A CELL FREE XYLOSE ISOMERASE ENZYME SORBED ON PARTICULATE BASIC MAGNESIUM CARBONATE.
Claim 3
Dependent Claim
21 words
The stabilized xylose isomerase enzyme preparation of claim 1, wherein said enzyme is derived from a microorganism of the genus Streptomyces.
Claim 4
Dependent Claim
40 words
A stabilized and immobilized xylose isomerase enzyme preparation comprising cell-free xylose isomerase bound to particulate basic magnesium carbonate, said preparation being characterized as having an effective isomerase activity of at least about 100 units per gram of the enzyme preparation.
Claim 5
Dependent Claim
40 words
A stabilized and immobilized xylose isomerase enzyme preparation comprising cell-free xylose isomerase bound to particulate basic magnesium carbonate, said preparation being characterized as having an effective isomerase activity of at least about 100 units per gram of the enzyme preparation.
Claim 6
Dependent Claim
15 words
The preparation of claim 3, wherein said particulate basic magnesium carbonate is in granular form.
Claim 7
Dependent Claim
19 words
The preparation of claim 3, wherein said soluble xylose isomerase is derived from a microorganism of the genus Streptomyces.
Claim 8
Dependent Claim
39 words
The preparation of claim 3, wherein said soluble xylose isomerase is derived from a microorganism selected from the group of strains consisting of S. olivochromogenes, ATCC No. 21713, S. olivochromogenes, ATCC No. 21714, and S. olivochromogenes, ATCC No. 21715.
Claim 9
Dependent Claim
28 words
The preparation of claim 3, wherein said composition has an effective isomerase activity of at least about 175 units of isomerase activity per gram of the enzyme preparation.
Claim 10
Dependent Claim
30 words
A process for the preparation of a stabilized and immobilized xylose isomerase enzyme preparation comprising contacting a particulate basic magnesium carbonate with a solution containing cell-free, soluble xylose isomerase enzyme.
Claim 11
Dependent Claim
30 words
A process for the preparation of a stabilized and immobilized xylose isomerase enzyme preparation comprising contacting a particulate basic magnesium carbonate with a solution containing cell-free, soluble xylose isomerase enzyme.
Claim 12
Dependent Claim
22 words
The process of claim 8, wherein the pH of the solution is in the range of from about 7.5 to about 9.5.
Claim 13
Dependent Claim
18 words
The process of claim 8, wherein the cell-free and soluble xylose isomerase enzyme is in substantially purified form.
Claim 14
Dependent Claim
21 words
The process of claim 8, wherein the cell-free and soluble xylose isomerase is derived from a microorganism of the genus Streptomyces.
Claim 15
Dependent Claim
38 words
The process of claim 8, wherein the cell-free and soluble xylose isomerase is derived from a microorganism of the strains consisting of S. olivochromogenes, ATCC No. 21713, S. olivochromogenes, ATCC No. 21714, and S. olivochromogenes, ATCC No. 21715.
Claim 16
Dependent Claim
85 words
A process for enzymatically converting dextrose to levulose which comprises passing a reaction solution containing from about 20 percent to about 70 percent dextrose, by weight, at a pH in the range from about 7.5 to about 9.5, and at a temperature of from about 30* C to about 90* C, through a bed of an enzyme preparation of cell-free xylose isomerase bound to particulate basic magnesium carbonate having an effective isomerase activity of at least about 100 units per gram of the enzyme preparation.
Claim 17
Dependent Claim
85 words
A process for enzymatically converting dextrose to levulose which comprises passing a reaction solution containing from about 20 percent to about 70 percent dextrose, by weight, at a pH in the range from about 7.5 to about 9.5, and at a temperature of from about 30* C to about 90* C, through a bed of an enzyme preparation of cell-free xylose isomerase bound to particulate basic magnesium carbonate having an effective isomerase activity of at least about 100 units per gram of the enzyme preparation.
Claim 18
Dependent Claim
38 words
The process of claim 13, whereiN said reaction solution contains from about 40 percent to about 60 percent dextrose by weight and the pH of the solution is in the range of from about 8.2 to about 9.2.
Claim 19
Dependent Claim
36 words
The process of claim 13, wherein said bed containing solubilized xylose isomerase bound to a particulate basic magnesium carbonate has an effective isomerase activity of at least about 175 units per gram of the enzyme preparation.
Claim 20
Dependent Claim
20 words
The process of claim 13, wherein said xylose isomerase enzyme is derived from a microorganism belonging to the Streptomyces genus.
Claim 21
Dependent Claim
39 words
The process of claim 13, wherein the soluble xylose isomerase is derived from a microorganism selected from the group of strains consisting of S. olivochromogenes, ATCC No. 21713, S. olivochromogenes, ATCC No. 21714, and S. olivochromogenes, ATCC No. 21715.
Claim 22
Dependent Claim
23 words
The process of claim 13, wherein said bed containing soluble xylose isomerase bound to a particulate basic magnesium carbonate is in a column.
Claim 23
Dependent Claim
27 words
The process of claim 18, wherein said column contains inlets whereby an alkalizing agent is fed into the column to regulate the pH of the reaction solution.
Claim 24
Dependent Claim
20 words
The process of claim 18, wherein said reaction solution is continuously passed through more than one column positioned in series.
Claim 25
Dependent Claim
15 words
The process of claim 13, wherein said particulate basic magnesium carbonate is in granular form.
Claim 26
Dependent Claim
26 words
The process of claim 13, wherein the reaction solution is a starch hydrolysate produced by either sequentially or concurrently enzymatically liquefying and saccharifying a starch-containing solution.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-11-11 | 1978-05-13 | 1978-11-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-11-11 | 1982-05-13 | 1982-11-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-11-11 | 1986-05-13 | 1986-11-12 | $7700.00 | Window Closed / Lapsed |