Patent US3963576A successfully refreshed from Google Patents.
US3963576A
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Method for rendering bacteria dormant and the product produced thereby
Assignee: General Environmental Science Corp
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Filing: 1975-02-21
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Granted: 1976-06-15
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Expires: 1995-02-21
Abstract
Abstract A method for rendering bacteria dormant is provided which includes the steps of growing bacteria under aerobic conditions in an aqueous media in such a manner that the so-produced bacteria include at least one species of Pseudemonas bacteria which is capable of enzymatically reducing nitrate to nitrogen and one species of Rhodopseudomonas bacteria which is capable of photosynthetically forming red pigment; dissolving an effective amount of at least one compound selected from the group consisting of the sulfides of sodium and potassium in the bacteria containing media; and exposing the sulfide containing media under anaerobic conditions to light for a period of time sufficient to cause red photosynthetic pigment to develop in the Rhodopseudomonas bacteria. The product produced is a suspension of dormant bacteria.
Inventors
- 👤 Frank L. Horsfall, III
- 👤 Barton Gilbert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 09:20
CPC Classifications
C; C02; C02F; C02F3/00; C02F3/34; C12; C12N; C12N1/00; C12N1/04; C12N1/20; C12N1/205; C12P; C12P39/00; C12R; C12R2001/00; C12R2001/01; C12R2001/38; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/874
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
119 words
A method for rendering an aqueous suspension of bacteria dormant which comprises, a. growing bacteria under aerobic conditions in an aqueous media in such a manner that said bacteria includes at least one species of Pseudemonas bacteria which is capable of enzymatically reducing nitrate to nitrogen and one species of Rhodopseudomonas bacteria which is capable of photosynthetically forming red pigment; b. dissolving an effective amount of at least one sulfide compound selected from the group consisting of the sulfides of sodium, potassium and mixtures thereof in said bacteria containing media; and c. exposing said sulfide containing media under anaerobic conditions to light for a period of time sufficient to cause red photosynthetic pigment to develop in the Rhodopseudomonas bacteria.
Claim 2
Independent Claim
119 words
A method for rendering an aqueous suspension of bacteria dormant which comprises, a. growing bacteria under aerobic conditions in an aqueous media in such a manner that said bacteria includes at least one species of Pseudemonas bacteria which is capable of enzymatically reducing nitrate to nitrogen and one species of Rhodopseudomonas bacteria which is capable of photosynthetically forming red pigment; b. dissolving an effective amount of at least one sulfide compound selected from the group consisting of the sulfides of sodium, potassium and mixtures thereof in said bacteria containing media; and c. exposing said sulfide containing media under anaerobic conditions to light for a period of time sufficient to cause red photosynthetic pigment to develop in the Rhodopseudomonas bacteria.
Claim 3
Dependent Claim
30 words
The method of claim 1 wherein the concentration of sulfide compound, based on the sulfide (sulfur) content of said compound, ranges from about 0.07 to about 0.3 grams per liter.
Claim 4
Dependent Claim
12 words
The method of claim 2 wherein said sulfide compound is sodium sulfide.
Claim 5
Dependent Claim
32 words
The method of claim 3 wherein said bacteria are grown using a nutrient system comprising: a. ammonium chloride, b. monobasic potassium phosphate; c. magnesium sulfate; d. sodium acetate, and e. yeast extract.
Claim 6
Dependent Claim
15 words
The method of claim 4 wherein the bacteria grown further includes Bacillus and Pseudomonas bacteria.
Claim 7
Dependent Claim
26 words
The method of claim 1 wherein the concentration of cells grown in step (a) is at least 1 × 10 6 cells per milliliter of solution.
Claim 8
Dependent Claim
49 words
The method of claim 5 wherein said bacteria are grown at a temperature ranging from about 25° to about 30°C for a period of about 18 to 24 hours, whereby the total cell concentration of bacteria ranges from about 2 to about 5 million cells per milliliter of solution.
Claim 9
Dependent Claim
17 words
The method of claim 7 wherein the amount of sodium sulfide is about 1.0 grams per liter.
Claim 10
Dependent Claim
25 words
The method of claim 1 wherein step (c) is continued for a period of time sufficient to cause the Rhodopseudomonas to develop photosynthetic red pigment.
Claim 11
Dependent Claim
17 words
The method of claim 9 wherein step (c) is continued for a period of about 24 hours.
Claim 12
Dependent Claim
115 words
An aqueous suspension of dormant bacteria obtained by: a. growing bacteria under aerobic conditions in an aqueous media in such a manner that said bacteria includes at least one species of Pseudemonas bacteria which is capable of enzymatically reducing nitrate to nitrogen and one species of Rhodopseudomonas bacteria which is capable of photosynthetically forming red pigment; b. dissolving an effective amount of at least one sulfide compound selected from the group consisting of the sulfides of sodium, potassium and mixtures thereof in said bacteria containing media; and c. exposing said sulfide containing media under anaerobic conditions to light for a period of time sufficient to cause red photosynthetic pigment to develop in the Rhodopseudomonas bacteria.
Claim 13
Dependent Claim
115 words
An aqueous suspension of dormant bacteria obtained by: a. growing bacteria under aerobic conditions in an aqueous media in such a manner that said bacteria includes at least one species of Pseudemonas bacteria which is capable of enzymatically reducing nitrate to nitrogen and one species of Rhodopseudomonas bacteria which is capable of photosynthetically forming red pigment; b. dissolving an effective amount of at least one sulfide compound selected from the group consisting of the sulfides of sodium, potassium and mixtures thereof in said bacteria containing media; and c. exposing said sulfide containing media under anaerobic conditions to light for a period of time sufficient to cause red photosynthetic pigment to develop in the Rhodopseudomonas bacteria.
Claim 14
Dependent Claim
31 words
The aqueous suspension of claim 11 wherein the concentration of sulfide compound, based on the sulfide (sulfur) content of said compound, ranges from about 0.07 to about 0.3 grams per liter.
Claim 15
Dependent Claim
13 words
The aqueous suspension of claim 12 wherein said sulfide compound is sodium sulfide.
Claim 16
Dependent Claim
33 words
The aqueous suspension of claim 13 wherein said bacteria are grown using a nutrient system comprising: a. ammonium chloride, b. monobasic potassium phosphate; c. magnesium sulfate; d. sodium acetate, and e. yeast extract.
Claim 17
Dependent Claim
16 words
The aqueous suspension of claim 14 wherein the bacteria grown further includes Bacillus and Pseudomonas bacteria.
Claim 18
Dependent Claim
27 words
The aqueous suspension of claim 11 wherein the concentration of cells grown in step (a) is at least 1 × 10 6 cells per milliliter of solution.
Claim 19
Dependent Claim
50 words
The aqueous suspension of claim 15 wherein said bacteria are grown at a temperature ranging from about 25° to about 30°C. for a period of about 18 to 24 hours, whereby the total cell concentration of bacteria ranges from about 2 to about 5 million cells per milliliter of solution.
Claim 20
Dependent Claim
18 words
The aqueous suspension of claim 17 wherein the amount of sodium sulfide is about 1.0 grams per liter.
Claim 21
Dependent Claim
26 words
The aqueous suspension of claim 11 wherein step (c) is continued for a period of time sufficient to cause the Rhodopseudomonas to develop photosynthetic red pigment.
Claim 22
Dependent Claim
18 words
The aqueous suspension of claim 19 wherein step (c) is continued for a period of about 24 hours.
References Cited (Backward Citations)
US3880740A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-15 | 1979-12-15 | 1980-06-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-15 | 1983-12-15 | 1984-06-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-15 | 1987-12-15 | 1988-06-15 | $7700.00 | Window Closed / Lapsed |