Patent US3926732A successfully refreshed from Google Patents.
US3926732A
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Method for assaying catalase in milk and other liquids
Assignee: Alfa Laval AB
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Filing: 1974-02-14
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Granted: 1975-12-16
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Expires: 1994-02-14
Abstract
Abstract To the milk or other catalase-containing liquid, mainly of biological origin, are added a reagent comprising a substance which releases hydrogen peroxide in the presence of a substance available in the milk or other liquid, and another reagent which, upon oxidation by hydrogen peroxide, yields a color reaction, the catalase content being determined qualitatively and quantitatively with the aid of the hue of the color thus produced. A device for carrying out this assay method comprises the two reagents separated physically from each other by a space through which the generated hydrogen peroxide can diffuse and which is capable of admitting the milk or other liquid.
Inventors
- 👤 Ernst Anders Carl-Gustaf Rosen
- 👤 Helena Mariana Rosen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:15
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/02; G01N33/04; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/30; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
99 words
A METHOD FOR QUALITATIVE AND QUANTITATIVE DETERMINATION OF CATALASE IN A LIQUID SAID METHOD COMPRISING THE STEPS OF ADDING TO THE LIQUID A FIRST REAGENT COMPRISING A SUBSTANCE WHICH RELEASES HYDROGEN PEROXIDE IN THE PRESENCE OF ANOTHER SUBSTANCE IN SAID LIQUID ADDING TO THE LIQUID A SECOND REAGENT OXIDIZABLE BY HYDROGEN PEROXIDE TO YIELD A COLOR REACTION, SAID OXIDATION BY HYDROGEN PEROXIDE BEING INHIBITED BY THE PRESENCE OF CATALASE, WHEREBY SAID COLOR REACTION IS STRONGER WITH A SMALLER CATALASE CONTENT OF THE LIQUID, AND DETERMINING THE CATALASE CONTENT WITH THE AID OF THE HUE OF THE COLOR THUS PRODUCED.
Claim 2
Independent Claim
99 words
A METHOD FOR QUALITATIVE AND QUANTITATIVE DETERMINATION OF CATALASE IN A LIQUID SAID METHOD COMPRISING THE STEPS OF ADDING TO THE LIQUID A FIRST REAGENT COMPRISING A SUBSTANCE WHICH RELEASES HYDROGEN PEROXIDE IN THE PRESENCE OF ANOTHER SUBSTANCE IN SAID LIQUID ADDING TO THE LIQUID A SECOND REAGENT OXIDIZABLE BY HYDROGEN PEROXIDE TO YIELD A COLOR REACTION, SAID OXIDATION BY HYDROGEN PEROXIDE BEING INHIBITED BY THE PRESENCE OF CATALASE, WHEREBY SAID COLOR REACTION IS STRONGER WITH A SMALLER CATALASE CONTENT OF THE LIQUID, AND DETERMINING THE CATALASE CONTENT WITH THE AID OF THE HUE OF THE COLOR THUS PRODUCED.
Claim 3
Dependent Claim
13 words
The method of claim 1, in which said first reagent is a peroxide.
Claim 4
Dependent Claim
34 words
The method of claim 1, in which said first reagent is a compound capable of generating a peroxide. 4.The method of claim 1, in which said other substance in the liquid is xanthine oxidase.
Claim 5
Dependent Claim
13 words
The method of claim 1, in which said first reagent includes an enzyme.
Claim 6
Dependent Claim
15 words
The method of claim 1, in which said first reagent is the enzyme galactose oxidase.
Claim 7
Dependent Claim
29 words
The method of claim 1, in which said substance of the first reagent is made up of synergistic substances consisting essentially of the enzymes Beta -galactoxidase and glucose oxidase.
Claim 8
Dependent Claim
29 words
The method of claim 1, in which said substance of the first reagent is made up of synergistic substances consisting essentially of the enzymes Beta -galactoxidase and galactose oxidase.
Claim 9
Dependent Claim
14 words
The method of claim 1, in which said second reagent is a leuko dye.
Claim 10
Dependent Claim
14 words
The method of claim 1, comprising also adding to said liquid the enzyme peroxidase.
Claim 11
Dependent Claim
34 words
The method of claim 1, wherein said two reagents are added while physically separated from each other by a space through which said released hydrogen peroxide diffuses and to which said liquid is admitted.
Claim 12
Dependent Claim
33 words
The method of claim 1, wherein said two reagents are added by maintaining the reagents separated from each other while attached to a porous body, and introducing the liquid into the porous body.
Claim 13
Dependent Claim
30 words
The method of claim 12, comprising also enclosing the two reagents separately in respective gel layers, and attaching the two gel layers apart from each other to the porous body.
Claim 14
Dependent Claim
30 words
The method of claim 12, comprising also adsorbing the two reagents separately in respective porous layers, and attaching the two porous layers apart from each other to the porous body.
Claim 15
Dependent Claim
34 words
The method of claim 12, comprising also separately microencapsulating each of the two reagents, forming a mixture of the separate microcapsules and an inert diffusion medium, and forming said mixture into said porous body.
Claim 16
Dependent Claim
23 words
The method of claim 12, comprising also separately microencapsulating each of the two reagents, and attaching the microcapsules separating to said porous body.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-15 | 1979-06-16 | 1979-12-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-15 | 1983-06-16 | 1983-12-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-15 | 1987-06-16 | 1987-12-16 | $7700.00 | Window Closed / Lapsed |