US3907637A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the production of L-lysine
Assignee: KH Neochem Co Ltd
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Filing: 1974-04-05
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Granted: 1975-09-23
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Expires: 1994-04-05
Abstract
Abstract L-lysine is produced by fermentation of a mutant strain of the genus Protaminobacter, capable of utilizing methanol and having a resistance to S-2-aminoethyl-L-cysteine and L-threonine. The mutant strain is cultured in a medium containing methanol as a main carbon source. L-lysine is accumulated in the culture medium and isolated therefrom.
Inventors
- 👤 Kiyoshi Nakayama
- 👤 Mamoru Kohata
- 👤 Yoshitake Tanaka
- 👤 Tadaaki Nomura
- 👤 Ryoichi Katsumata
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 20:09
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/08; Y; Y10; Y10S; Y10S435/00; Y10S435/813; Y10S435/8215; Y10S435/822; Y10S435/858
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
66 words
A PROCESS FOR THE PRODUCTION OF L-LYSINE WHICH COMPRISES CULTURING A MUTANT STRAIN BELONGING TO THE GENUS PROTAMINOBACTER, SAID MUTANT BEING CAPABLE OF UTILIZING METHANOL AND CAPABLE OF GROWING IN THE PRESENCE OF AT LEAST 10MG/ML OF S-2-AMINOETHYL-L-CYSTEIN AND AT LEAST 20MG/ML OF L-THREONINE, IN A NUTRIENT MEDIUM CONTAINING METHANOL AS A MAIN SOURCE OF CARBON, ACCUMULATING L-LYSINE IN SAID MEDIUM AND THEREAFTER ISOLATING SAID L-LYSINE.
Claim 2
Dependent Claim
66 words
A PROCESS FOR THE PRODUCTION OF L-LYSINE WHICH COMPRISES CULTURING A MUTANT STRAIN BELONGING TO THE GENUS PROTAMINOBACTER, SAID MUTANT BEING CAPABLE OF UTILIZING METHANOL AND CAPABLE OF GROWING IN THE PRESENCE OF AT LEAST 10MG/ML OF S-2-AMINOETHYL-L-CYSTEIN AND AT LEAST 20MG/ML OF L-THREONINE, IN A NUTRIENT MEDIUM CONTAINING METHANOL AS A MAIN SOURCE OF CARBON, ACCUMULATING L-LYSINE IN SAID MEDIUM AND THEREAFTER ISOLATING SAID L-LYSINE.
Claim 3
Dependent Claim
37 words
A process according to claim 1 wherein said methanol is fed to said culture medium continuously in an amount of 0.3 to 0.6% by volume based upon the volume of the culture medium during said culturing step.
Claim 4
Dependent Claim
37 words
A process according to claim 1 wherein said methanol is fed to said culture medium intermittently in an amount of 0.5 to 2% by volume based upon the volume of said culture medium during said culturing step.
Claim 5
Dependent Claim
15 words
A process according to claim 1, wherein said mutant belongs to the species Protaminobacter thiaminophagus.
Claim 6
Dependent Claim
14 words
A process according to claim 4, wherein said mutant is Protaminobacter thiaminophagus ATCC 21926.
Claim 7
Dependent Claim
15 words
A process according to claim 1, wherein culturing is carried out at 20* to 40*C.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-09-22 | 1979-03-24 | 1979-09-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-09-22 | 1983-03-24 | 1983-09-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-09-22 | 1987-03-24 | 1987-09-23 | $7700.00 | Window Closed / Lapsed |