Patent US3875002A successfully refreshed from Google Patents.
US3875002A
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Highly specific tuberculin
Assignee: Sanofi Pasteur Ltd
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Filing: 1974-01-14
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Granted: 1975-04-01
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Expires: 1994-01-14
Abstract
Abstract A purified highly specific tuberculin, substantially free of protein, useful in the diagnosis of tuberculosis in humans and a process for preparing said tuberculin. Unlike known tuberculins, the aforesaid tuberculin is capable of being used to distinguish between tuberculosis-infected individuals and other individuals such as BCG-vaccinated individuals, and individuals infected with atypical mycobacteria of Runyon Groups I, II, III and IV. The average molecular weight of the purified highly specific tuberculin is lower than known tuberculins and in one embodiment of the purified highly specific tuberculin the average molecular weight is not more than 5000.
Inventors
- 👤 Silvio Landi
- 👤 Krishana Chandara Gupta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 16:46
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/35; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
52 words
A PROCESS FOR THE PREPARATION OF A PURIFIED HIGHLY SPECIFIC TUBERCULIN PEPTIDE SUBSTANTIALLY FEED OF PROTEIN COMPRISING HYDROLYZING A TUBERCULIN PPD WITH AN ALKALINE ACTIVE PROTEOLYTIC ENZYME, REMOVING PROTEIN FROM THE RESULTANT HYDROLYSTATE AND RECOVERING A FIRST PEPTIDE FRACTION FROM THE REMAINING HYDROLYSATE BY SEPARATION WITH AN ION-EXCHANGE RESIN AT ABOUT PH3.
Claim 2
Dependent Claim
52 words
A PROCESS FOR THE PREPARATION OF A PURIFIED HIGHLY SPECIFIC TUBERCULIN PEPTIDE SUBSTANTIALLY FEED OF PROTEIN COMPRISING HYDROLYZING A TUBERCULIN PPD WITH AN ALKALINE ACTIVE PROTEOLYTIC ENZYME, REMOVING PROTEIN FROM THE RESULTANT HYDROLYSTATE AND RECOVERING A FIRST PEPTIDE FRACTION FROM THE REMAINING HYDROLYSATE BY SEPARATION WITH AN ION-EXCHANGE RESIN AT ABOUT PH3.
Claim 3
Dependent Claim
20 words
A process according to claim 1 wherein the enzyme is one of the group consisting of trypsin, chymotrypsin and ''proteinase''.
Claim 4
Dependent Claim
11 words
A process according to claim 1 wherein the enzyme is trypsin.
Claim 5
Dependent Claim
11 words
A process according to claim 1 wherein the enzyme is chymotrypsin.
Claim 6
Dependent Claim
11 words
A process according to claim 1 wherein the enzyme is ''proteinase''.
Claim 7
Dependent Claim
17 words
A process according to claim 1 wherein the protein is removed from the resultant hydrolysate by precipitation.
Claim 8
Dependent Claim
70 words
A process according to claim 6 in which the tuberculin peptide has an average molecular weight of not more than 5,000, wherein the protein is removed from the hydrolysate by removing a first precipitate with trichloroacetic acid and then by removinG a second precipitate from the resultant supernatant with phosphotungstic acid and recovering a peptide precipitate from an aqueous barium hydroxide solution of the second precipitate by precipitation with acetone.
Claim 9
Dependent Claim
16 words
A process according to claim 7 wherein the enzyme is selected from trypsin, chymotrypsin and ''proteinase''.
Claim 10
Dependent Claim
18 words
A purified highly specific tuberculin peptide substantially free of protein whenever prepared by the process of claim 1.
Claim 11
Dependent Claim
51 words
A process for the preparation of a purified highly specific tuberculin peptide substantially free of protein comprising hydrolyzing a tuberculin PPD with an alkaline active proteolytic enzyme, removing protein from the resultant hydrolysate and recovering a first peptide fraction from the remaining hydrolysate by separation with an ion-exchange resin at pH3.1.
Claim 12
Dependent Claim
51 words
A process for the preparation of a purified highly specific tuberculin peptide substantially free of protein comprising hydrolyzing a tuberculin PPD with an alkaline active proteolytic enzyme, removing protein from the resultant hydrolysate and recovering a first peptide fraction from the remaining hydrolysate by separation with an ion-exchange resin at pH3.1.
Claim 13
Dependent Claim
18 words
A purified highly specific tuberculin peptide substantially free of protein whenever prepared by the process of claim 10.
References Cited (Backward Citations)
US3621005A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-31 | 1978-09-30 | 1979-04-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-31 | 1982-09-30 | 1983-04-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-31 | 1986-09-30 | 1987-04-01 | $7700.00 | Window Closed / Lapsed |