Electrophoresis chamber

Claim 2

In electrophoresis apparatus comprising a pair of adjacent compartments each constructed and arranged to hold a supply of buffer solution, an electrode in each said compartment in position to be submerged in the respective supply of buffer solution and means for connecting said electrodes to a source of electrical potential, and means for supporting in position above the surface of said supplies of buffer solution an impervious backing member having an electrophoresis gel layer on its upper surface, the improvement comprising a cover for said compartments having an aperture positioned over the location of said gel layer arranged to permit introduction therethrough of material to be subjected to electrophoresis, said cover having secured to its bottom adjacent each opposite side of said aperture a layer of bibulous material in position to bear upon each opposing margin of said gel layer, each said layer of bibulous material extending laterally away from said aperture to a position beyond the margin of said underlying gel layer, thence downwardly into one of said compartments, each said layer of bibulous material being constructed and arranged to come into contact with its respective supply of buffer solution.

Claim 3

Apparatus as claimed in claim 1 including means for advancing said backing member with its gel layer along said supporting means.

Claim 4

Apparatus as claimed in claim 1 in which said supporting means for said backing member extends over said compartments and is provided with slots through which said layer of bibulous material extends downwardly.

Claim 5

In electrophoresis apparatus comprising a pair of adjacent compartments each constructed and arranged to hold a supply of buffer solution, the improvement comprising removable means for supporting in position above the surface of said supplies of buffer solution an impervious backing member having an electrophoresis gel layer on its upper surface, said supporting means including a pair of electrodes and means for supporting each said electrode in each of said compartments in posiTion to contact the respective supply of buffer solution and means for detachably connecting said electrodes to a source of electrical potential, and a cover for said compartments having an aperture positioned over the location of said gel layer arranged to permit introduction therethrough of material to be subjected to electrophoresis said cover having secured to its bottom adjacent each opposite side of said aperture a layer of bibulous material in position to bear upon each opposing margin of said gel layer, each said layer of bibulous material extending laterally away from said aperture to a position beyond the margin of said underlying gel layer, thence downwardly into one of said compartments, each said layer of bibulous material being constructed and arranged to come into contact with its respective supply of buffer solution.

Claim 6

In electrophoresis apparatus comprising a pair of adjacent compartments each constructed and arranged to hold a supply of buffer solution, the improvement comprising removable means for supporting in position above the surface of said supplies of buffer solution an impervious backing member having an electrophoresis gel layer on its upper surface, said supporting means including a pair of electrodes and means for supporting each said electrode in each of said compartments in posiTion to contact the respective supply of buffer solution and means for detachably connecting said electrodes to a source of electrical potential, and a cover for said compartments having an aperture positioned over the location of said gel layer arranged to permit introduction therethrough of material to be subjected to electrophoresis said cover having secured to its bottom adjacent each opposite side of said aperture a layer of bibulous material in position to bear upon each opposing margin of said gel layer, each said layer of bibulous material extending laterally away from said aperture to a position beyond the margin of said underlying gel layer, thence downwardly into one of said compartments, each said layer of bibulous material being constructed and arranged to come into contact with its respective supply of buffer solution.

Claim 7

Apparatus as claimed in claim 4 in which the means for supporting each said electrode comprises a baffle extending downwardly into each compartment.

Claim 8

Apparatus as claimed in claim 5 in which the detachable electrical connection means comprises a connection between said support means and the wall of the compartment.