US3674678A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrophoretic apparatus
Assignee: EMD Millipore Corp
•
Filing: 1970-10-28
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Granted: 1972-07-04
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Expires: 1990-10-28
Abstract
Abstract An apparatus for conducting electrophoretic analysis comprising a container filled with a conductive gel, two electrodes in the container and means for effecting a voltage across the gel. The container is shaped with deep reservoirs at opposing ends and a relatively shallow mid-section having wells to accommodate a sample to be analyzed. The electrodes extend the width of the reservoirs at about the reservoir midpoint. This apparatus eliminates the need for wicks in gel electrophoresis.
Inventors
- 👤 Arthur H Post Jr
- 👤 Ronald L Polinsky
- 👤 James L Dwyer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 05:03
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
73 words
An apparatus for conducting electrophoretic analysis comprising a hollow container, said container having deep reservoirs at opposing ends and a relatively shallow mid-section between said reservoirs, a continuous solid electrically conductive gel filling said reservoirs and covering said midsection with a relatively thin layer thereof, said gel remaining in a solid condition during use of the apparatus for conducting electrophoretic analysis, and an electrode embedded within the gel in each of said reservoirs.
Claim 2
Independent Claim
73 words
An apparatus for conducting electrophoretic analysis comprising a hollow container, said container having deep reservoirs at opposing ends and a relatively shallow mid-section between said reservoirs, a continuous solid electrically conductive gel filling said reservoirs and covering said midsection with a relatively thin layer thereof, said gel remaining in a solid condition during use of the apparatus for conducting electrophoretic analysis, and an electrode embedded within the gel in each of said reservoirs.
Claim 3
Dependent Claim
23 words
The apparatus of claim 1 wherein said electrodes are provided with means to prevent gas build-up at the electrode and to promote endosmosis.
Claim 4
Dependent Claim
31 words
The apparatus of claim 1 wherein wells in the mid-section of said gel are arranged in two separated parallel rows, the wells in each row being equidistant from an adjacent reservoir.
Claim 5
Dependent Claim
29 words
The apparatus of claim 2 wherein the net width of the gel mid-section between the rows of perforations is less than the width of the gel in each reservoir.
Claim 6
Dependent Claim
10 words
The apparatus of claim 1 wherein the container is transparent.
Claim 7
Dependent Claim
27 words
The apparatus of claim 1 wherein the container has walls extending above the surface of said gel and a water impermeable cover adapted to enclose the container.
Claim 8
Dependent Claim
23 words
The apparatus of claim 1 wherein the gel in the reservoirs adjacent the electrodes is perforated to permit escape of gases during electrophoresis.
Claim 9
Dependent Claim
17 words
The apparatus of claim 1 wherein the container has raised sections between adjacent wells in each row.
References Cited (Backward Citations)
US3317417A
Cited
US3371027A
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US3432414A
Cited
US3407133A
Cited
US3482943A
Cited
US3479265A
Cited
US3554894A
Cited
US3494846A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-07-04 | 1976-01-03 | 1976-07-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-07-04 | 1980-01-03 | 1980-07-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-07-04 | 1984-01-03 | 1984-07-04 | $7700.00 | Window Closed / Lapsed |