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US3755082A
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Methanol-assimilating propagation of microbial cells
Assignee: GYOZO TERUI JA
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Filing: 1971-11-10
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Granted: 1973-08-28
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Expires: 1991-11-10
Abstract
Abstract A strain belonging to the new species Pseudomonas methanolica is aerobically cultured in a nutrient medium containing methanol as a substrate and the propagated microbial cells are recovered from the culture liquor.
Inventors
- 👤 G Terui
- 👤 N Takada
- 👤 H Sawada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 13:26
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/32; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
16 words
The process of claim 1 wherein said strain of Pseudomonas methanolica is Pseudomonas methanolica ATCC 21704.
Claim 2
Dependent Claim
37 words
The process of claim 1 wherein said culturing is carried out in a single tank fermenter and said process further includes the step of maintaining the concentration of methanol in said culture medium stationarily below 0.2% (V/V).
Claim 3
Dependent Claim
64 words
the process of claim 1 wherein said culturing step includes the steps of culturing said strain in a main culturing tank containing a culture medium having a methanol concentration of from 1 to 2% (V/V), and venting volatilized methanol into a secondary culturing tank containing an inoculated culture medium having a methanol concentration less than said main tank to effect complete recovery of methanol.
Claim 4
Dependent Claim
43 words
The process of claim 1 wherein said culturing step includes the steps of continuously supplying culture medium to a culturing tank and partially discharging culture liquor from said tank while maintaining the methanol concentration in said culturing tank to less than 0.2% (V/V).
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-08-27 | 1977-02-26 | 1977-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-08-27 | 1981-02-26 | 1981-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-08-27 | 1985-02-26 | 1985-08-28 | $7700.00 | Window Closed / Lapsed |