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US3635796A Expired / Lapsed US ⏱️ 0.0 years remaining

Method for fermenting a liquefied hydrocarbon gas

Assignee: KH Neochem Co Ltd
•
Filing: 1968-05-26
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Granted: 1972-01-18
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Expires: 1988-05-26
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Abstract

Abstract A continuous process for culturing a micro-organism in an aqueous nutrient medium under aerobic conditions in the presence of a liquefied hydrocarbon gas as a main carbon source which comprises adding fresh liquefied hydrocarbon gas to the culture medium, determining the concentration of dissolved liquefied hydrocarbon gas in the culture medium and controlling the addition of fresh liquefied hydrocarbon gas depending upon the detected concentration of said liquefied hydrocarbon gas in the culture medium, the supply and dissolution of the liquefied hydrocarbon gas being based on the rate of said gas consumed by the micro-organisms. Alternatively, the partial pressure of the liquefied hydrocarbon gas in the vent gas can be measured and used to control the rate of liquefied hydrocarbon gas supplied to the culture medium.

Inventors

  • 👤 Osamu Imada
  • 👤 Kazuo Hoshiai
  • 👤 Masatami Tanaka

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-06 12:00
CPC Classifications
C; C12; C12M; C12M27/00; C12M27/02; C12M27/08; C12M27/18; C12M27/24; C12M29/00; C12M29/18; C12M29/20; C12M41/00; C12M41/30; C12M41/32; C12N; C12N1/00; C12N1/26; C12N1/28; C12N1/30; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/911; Y10S435/921

Claims Analysis

Total Claims: 6
Claim 1
Independent Claim 21 words
The process of claim 1 wherein, the micro-organism is selected from the group consisting of yeasts, bacteria, molds, actinomycetes, and fungi.
Claim 2
Dependent Claim 33 words
The process of claim 1 wherein, the micro-organism is a member selected from the group consisting of Candida rigida, No. 113, Candida japonica, No. 104, ATCC No. 14437 and Brevibacterium ketoglutamicum ATCC 15587.
Claim 3
Dependent Claim 25 words
The process of claim 1 wherein, the liquefied hydrocarbon gas is selected from the group consisting of methane, ethane, propane, n-butane, iso-butane, propylene, and butylene.
Claim 4
Dependent Claim 21 words
The process of claim 1 wherein, the liquefied hydrocarbon gas is mixed with air, or a mixture of oxygen and air.
Claim 5
Dependent Claim 155 words
A continuous process for culturing a micro-organism selected from the group consisting of Candida rigida, No. 113, Candida japonica, No. 104, ATCC 14437 and Brevibacterium ketoglutamicum ATCC 15587 in an aqueous nutrient medium under aerobic conditions in the presence of a liquefied hydrocarbon gas as the main carbon source which comprises adding fresh liquefied hydrocarbon gas to the culture medium, conducting the culturing at a temperature of about 20* to 40* C. and pH of about 5 to 9, determining the concentration of dissolved liquefied hydrocarbon gas in the culture medium by measuring the partial pressure of the liquefied hydrocarbon gas in the vent which is removed from the culture medium, and controlling the addition of fresh liquefied hydrocarbon gas to the medium depending upon the measurement of liquefied hydrocarbon gas in the vent, the supply and dissolution of liquefied hydrocarbon gas being based on the rate of liquefied hydrocarbon gas consumed by the micro-organisms.
Claim 6
Dependent Claim 25 words
The process of claim 6, wherein the liquefied hydrocarbon gas is selected from the group consisting of methane, ethane, propane, n-butane, iso-butane, propylene, and butylene.

References Cited (Backward Citations)

US3010881A Cited
US3222258A Cited
US3384553A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1975-01-17 1975-07-19 1976-01-18 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1979-01-17 1979-07-19 1980-01-18 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1983-01-17 1983-07-19 1984-01-18 $7700.00 Window Closed / Lapsed

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