Patent US3558066A successfully refreshed from Google Patents.
US3558066A
Expired / Lapsed
US
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Ultrasonic extraction of viable antigens from gram positive bacteria
Assignee: Individual
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Filing: 1969-01-21
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Granted: 1971-01-26
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Expires: 1989-01-21
Abstract
Abstract A method of extracting antigens from Gram Positive bacteria cells including injecting ultrasonic energy into a solution of live Gram Positive bacteria contained in a narrow cylindrical vessel cooled by a cooling bath maintained at a temperature less than 0*C. After immersing the tip of an ultrasonic probe into the solution, the probe is energized and the solution is maintained at a temperature close to 0*C. The tip of the probe is removed from the solution after an interval of time necessary to release the appropriate antigens or enzymes from said bacteria cells, and thereafter the antigens are separated, usually as a solution in the medium from the residue of the bacteria cells.
Inventors
- 👤 Howard Alliger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 06:16
CPC Classifications
A; A61; A61K; A61K41/00; A61K41/0028; A61K39/00
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
33 words
A method according to claim 1, wherein said solution of bacteria cells is cooled by a cooling bath maintained at a temperature of less than 0* C. while said ultrasonic probe is energized.
Claim 2
Dependent Claim
32 words
A method according to claim 2, wherein the temperature of said solution during the energizing of said ultrasonic probe is maintained in the range of between above 0* C. and 5* C.
Claim 3
Dependent Claim
35 words
A method according to claim 3, wherein said antigenic substances are separated from the residue of said bacteria cells by filtering said solution through a filter having a porosity on the order of 0.3 microns.
Claim 4
Dependent Claim
43 words
A method according to claim 4, including the step of separating antigenic substances as a solution in said medium from said residue of said bacteria cells prior to the filtering thereof by centrifuging to remove at least the major portion of said residue.
Claim 5
Dependent Claim
18 words
A method according to claim 1, wherein said ultrasonic energy is introduced into said solution of live bacteria.
Claim 6
Dependent Claim
58 words
A method according to claim 1, wherein said glass powder has a particle size in the range of about 1 to about 100 microns and said ultrasonic probe operates in the range of about 10 to about 100 kHz. and the power at said tip of said probe has an intensity of approximately 1,000 watts per square inch.
Claim 7
Dependent Claim
16 words
A Method according to claim 1, wherein said Gram Positive bacteria cells are staphlococcus strain 2902.
Claim 8
Dependent Claim
64 words
A method according to claim 8, wherein said glass powder has a particle size in the range of about 1 to about 10 microns and said ultrasonic probe operates at about 20 kHz. and at an intensity of approximately 1,000 watts per square inch for a period of approximately 3 hours to remove enzymes from between the outer and inner walls of said staphlococcus.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-01-25 | 1974-07-27 | 1975-01-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-01-25 | 1978-07-27 | 1979-01-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-01-25 | 1982-07-27 | 1983-01-26 | $7700.00 | Window Closed / Lapsed |