Method for preparing reticulo-endo-thelial system stimulant and stimulant thereof

Claim 1

IN A PROCESS FOR ISOLATING WALL COMPONENTS FROM MYCOBACTERIUM PHLEI WHICH COMPRISES: (A) RUPTURING SAID M. PHLEI BY FEEDING A VIABLE SUSPENSION OF SAID M. PHLEI IN AN AQUEOUS VEHICLE CONTAINING AT LEAST ONE SURFACE ACTIVE AGENT AND DISCRETE PARTICLES OF AN INERT MATERIAL HAVING A MAXIMUM AVERAGE DIMENSION OF ABOUT 120 MICRONS THROUGH AN OPENING OF NOT MORE THAN 0.03 INCH FORMED BY TWO SURFACES MOVING AT HIGH SPEED AND IN OPPOSING DIRECTIONS WITH RESPECT TO EACH OTHER; (B) REMOVING THE RESULTING SUSPENSION OF SAID RUPTURED M. PHLEI FROM SAID OPENING; (C) SUBJECTING SAID RUPTURED M. PHELI TO A GRAVITATIONAL FORCE OF ABOUT 9,200 TO 11,000 TIMES GRAVITY TO OBTAIN A CLEAR SUPERNATANT AND A SEDIMENTARY PHASE; (D) DISCARDING SAID CLEAR SUPERNATANT; (E) SUSPENDING SAID SEMIDENTARY PHASE WITH WATER CONTAINING AT LEAST ONE SURFACED ACTIVE AGENT; (F) SUBJECTING SAID SUSPENDED SEDIMENTARY PHASE TO A SECOND GRAVITATIONAL FORCE OF ABOUT 1,000 TO 1,200 TIMES GRAVITY TO OBTAIN A SUPERNATANT CONTAINING ISOLATED CELL WALL FRACTIONS OF SAID M. PHLEI AND SEDIMENTARY PHASE CONTAINING ANY INTACT CELLS OF SAID M. PHLEI; (G) DISCARDING SAID SEDIMENTARY PHASE CONTAINING INTACT CELLS, AND (H) PURIFYING SAID SUPERNATANT FLUID CONTAINING ISOLATED CELL WALL FRACTIONS OF M. PHLEI BY DIGESTING WITH RIBONUCLEASE AT A TEMPERATURE OF 37* TO 38*C., SEPARATING THE RIBONUCLEASE DIGESTED CELL WALL FRACTION FROM RIBONUCLEASE, DIGESTING SAID RIBONUCLEASE DIGESTED CELL WALL FRACTION WITH TRYPSIN AT A TEMPERATURE OF 37* TO 38*C. AND SEPARATING THE RESULTING TRYSPIN DIGESTED CELL WALL FRACTION FROM TRYPSIN.