Multifunctional laryngotracheitis vaccine and method of making the same

Claim 1

A PROCESS FOR PREPARING AN ATTENUATED LARYNGOTRACHEITIS VIRUS VACCINE WHICH CAN BE SAFELY ADMINISTERED OCULARLY, INTRANASALLY, INTRATRACHEALLY AND IN DRINKING WATER FO IMMUNIZING FOWL AGAINST INFECTIOUS LARYNGOTRACHEITIS COMPRISING THE STEPS OF INTRODUCING A VIRULENT, INFECTIOUS LARYNGOTRACHEITIS VIRUS INTO A FERTILE AVIAN EGG, INCUBATING THE EGG AT GROWTH SUSTAINING TEMPERATURES UNTIL A HARVESTABLE QUANTITY OF LARYNGOTRACHEITIS VIRUS HAS GROWN THEREIN, HARVESTING SAID LARYNGOTRACHEITIS VIRUS BY REMOVING AN INFECTED PORTION OF THE EMBRYONIC CONTENT OF THE EGG, REINTRODUCING THE VIRUS SO HARVESTED INTO A SECOND FERTILE AVIAN EGG AND REPEATING SUCH CULTURE PASSAGE OF THE VIRUS FOR A SUFFICIENT NUMBER OF PASSAGES TO PRODUCE AN ATTENUATED ANTIGENICALLY-EFFECTIVE LARYNGOTRACHEITIS VIRUS WHICH CAN BE SAFELY ADMINISTERED OCULARLY, INTRANASALLY, INTRATRACHEALLY AND IN DRINKING WATER.