US20030190748A1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Serum free cultivation of primate embryonic stem cells
Assignee: Wisconsin Alumni Research Foundation
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Filing: 2003-05-06
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Granted: —
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Expires: 2023-05-06
Abstract
Abstract Disclosed herein are methods for culturing primate embryonic stem cells. These cells are cultured on a prolonged and stable basis in the presence of exogenously supplied fibroblast growth factor and in the absence of animal serum. Preferably there is also a fibroblast feeder layer. Also disclosed is a culture media containing fibroblast feeder layer and the fibroblast growth factor.
Inventors
- 👤 James Thomson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 03:49
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0603; C12N5/0606; C12N2500/00; C12N2500/90; C12N2501/00; C12N2501/10; C12N2501/115
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
45 words
1 . A method of culturing primate embryonic stem cells, comprising: culturing the stem cells in a culture essentially free of mammalian fetal serum and in the presence of fibroblast growth factor that is supplied from a source other than just a fibroblast feeder layer.
Claim 2
Dependent Claim
45 words
1 . A method of culturing primate embryonic stem cells, comprising: culturing the stem cells in a culture essentially free of mammalian fetal serum and in the presence of fibroblast growth factor that is supplied from a source other than just a fibroblast feeder layer.
Claim 3
Dependent Claim
18 words
2 . The method of claim 1 , wherein the culture is essentially free of any animal serum.
Claim 4
Dependent Claim
17 words
3 . The method of claim 2 , wherein the culture also comprises a fibroblast feeder layer.
Claim 5
Dependent Claim
17 words
4 The method of claim 2 , wherein the fibroblast growth factor is basic fibroblast growth factor.
Claim 6
Dependent Claim
27 words
5 . The method of claim 4 , wherein the fibroblast growth factor is human basic fibroblast growth factor which has been produced from a recombinant gene.
Claim 7
Dependent Claim
19 words
6 . The method of claim 2 , wherein the primate embryonic stem cells are human embryonic stem cells.
Claim 8
Dependent Claim
51 words
7 . The method of claim 2 , wherein said culturing step includes the embryonic stem cells proliferating in culture for over one month while maintaining the potential of the stem cells to differentiate into derivatives of endoderm, mesoderm, and ectoderm tissues, and while maintaining the karyotype of the stem cells.
Claim 9
Dependent Claim
36 words
8 . The method of claim 2 , wherein the human basic fibroblast growth factor is present in the culture in a concentration of at least 0.1 ng/ml for at least a portion of the method.
Claim 10
Dependent Claim
57 words
9 . A method of culturing primate embryonic stem cells, comprising: culturing the stem cells in a culture essentially free of mammalian fetal serum and in the presence of a growth factor capable of activating a fibroblast growth factor signaling receptor, wherein the growth factor is supplied from a source other than just a fibroblast feeder layer.
Claim 11
Dependent Claim
57 words
9 . A method of culturing primate embryonic stem cells, comprising: culturing the stem cells in a culture essentially free of mammalian fetal serum and in the presence of a growth factor capable of activating a fibroblast growth factor signaling receptor, wherein the growth factor is supplied from a source other than just a fibroblast feeder layer.
Claim 12
Dependent Claim
18 words
10 . The method of claim 9 , wherein the culture is essentially free of any animal serum.
Claim 13
Dependent Claim
17 words
11 . The method of claim 10 , wherein the culture also comprises a fibroblast feeder layer.
Claim 14
Dependent Claim
19 words
12 . The method of claim 10 , wherein the primate embryonic stem cells are human embryonic stem cells.
Claim 15
Dependent Claim
51 words
13 . The method of claim 10 , wherein said culturing step includes the embryonic stem cells proliferating in culture for over one month while maintaining the potential of the stem cells to differentiate into derivatives of endoderm, mesoderm, and ectoderm tissues, and while maintaining the katyotype of the stem cells.
Claim 16
Dependent Claim
38 words
14 . A culture system for culturing primate embryonic stem cells, comprising: a fibroblast feeder layer; and fibroblast growth factor supplied by other than just the fibroblast layer; wherein the culture system is essentially free of animal serum.
Claim 17
Dependent Claim
38 words
14 . A culture system for culturing primate embryonic stem cells, comprising: a fibroblast feeder layer; and fibroblast growth factor supplied by other than just the fibroblast layer; wherein the culture system is essentially free of animal serum.
Claim 18
Dependent Claim
13 words
15 . A cell line derived using the method of claim 1 .
Claim 19
Dependent Claim
12 words
16 . A cell line derived using the method of claim 9.
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