US5690926A
Expired / Lapsed
US
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Pluripotential embryonic cells and methods of making same
Assignee: Vanderbilt University
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Filing: 1994-03-25
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Granted: 1997-11-25
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Expires: 2014-03-25
Abstract
Abstract The claimed invention is directed towards non-murine pluripotential cells that have the ability to be passaged in vitro for at least 20 passages and which differentiate in culture into a variety of tissues. The scope of the claimed cells includes any non-murine ES cells and particular claims are drawn to human pluripotential cells.
Inventors
- 👤 Brigid L. M. Hogan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 05:02
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0603; C12N5/0606; C12N2501/00; C12N2501/10; C12N2501/115; C12N2501/125; C12N2501/20; C12N2501/23; C12N2501/235; C12N2503/00; C12N2503/02; C12N2506/00; C12N2506/04; C12N2510/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
100 words
An isolated non-murine mammalian pluripotential cell wherein said cell exhibits the following characteristics: (a) can be maintained on feeder layers for at least 20 passages; and (b) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and wherein said cell is derived from a primordial germ cell by the process of: (1) culturing a non-murine mammalian primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor; (2) selecting cells that have characteristics (a) and (b), above, and (3) isolating said non-murine pluripotential cell.
Claim 2
Dependent Claim
100 words
An isolated non-murine mammalian pluripotential cell wherein said cell exhibits the following characteristics: (a) can be maintained on feeder layers for at least 20 passages; and (b) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and wherein said cell is derived from a primordial germ cell by the process of: (1) culturing a non-murine mammalian primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor; (2) selecting cells that have characteristics (a) and (b), above, and (3) isolating said non-murine pluripotential cell.
Claim 3
Dependent Claim
14 words
The pluripotential cell of claim 1, having a mutation which renders a gone non-functional.
Claim 4
Dependent Claim
13 words
The pluripotential cell of claim 1, having an insertion of a functional gene.
Claim 5
Dependent Claim
57 words
A method of using a non-murine and non-human pluripotential cell of claim 1 to contribute to chimeras in vivo comprising: injecting the cell into a blastocyst of the same species of said cell; implanting the resultant chimeric blastocyst into a foster mother of the same species; and allowing the chimeric blastocyst to grow within the foster mother.
Claim 6
Dependent Claim
59 words
A method of using a non-murine and non-human pluripotential cell of claim 1 to contribute to chimeras in vivo comprising: aggregating the cell with a morula stage embryo of the same species of said cell; implanting the resultant chimeric embryo into a foster mother of the same species; and allowing the chimeric embryo to grow within the foster mother.
Claim 7
Dependent Claim
99 words
An isolated human pluripotential cell wherein said cell exhibits the following characteristics: (a) can be maintained on feeder layers for at least 20 passages; and (b) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and wherein said cell is derived from a human primordial germ cell by the process of: (1) culturing a human primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor; (2) selecting cells that have characteristics (a) and (b), above, and (3) isolating said human pluripotential cell.
Claim 8
Dependent Claim
99 words
An isolated human pluripotential cell wherein said cell exhibits the following characteristics: (a) can be maintained on feeder layers for at least 20 passages; and (b) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and wherein said cell is derived from a human primordial germ cell by the process of: (1) culturing a human primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor; (2) selecting cells that have characteristics (a) and (b), above, and (3) isolating said human pluripotential cell.
Claim 9
Dependent Claim
135 words
A composition comprising: (A) a human pluripotential cell derived from a primordial germ cell wherein said cell exhibits the following characteristics: (1) can be maintained on feeder layers for at least 20 passages; and (2) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and wherein said cell is derived from a primordial germ cell by the process of: (a) culturing a human primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor; (b) selecting cells that have characteristics (a) and (b), above, and (c) isolating said human pluripotential cell; and (B) a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor, each in amounts sufficient to permit continued proliferation of said cell.
Claim 10
Dependent Claim
135 words
A composition comprising: (A) a human pluripotential cell derived from a primordial germ cell wherein said cell exhibits the following characteristics: (1) can be maintained on feeder layers for at least 20 passages; and (2) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture; and wherein said cell is derived from a primordial germ cell by the process of: (a) culturing a human primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor; (b) selecting cells that have characteristics (a) and (b), above, and (c) isolating said human pluripotential cell; and (B) a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor, each in amounts sufficient to permit continued proliferation of said cell.
References Cited (Backward Citations)
US5166065A
Cited
WO1990003432A1
Cited
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