US5670372A
Expired / Lapsed
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Pluripotential embryonic stem cells and methods of making same
Assignee: Vanderbilt University
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Filing: 1995-06-05
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Granted: 1997-09-23
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Expires: 2015-06-05
Abstract
Abstract The present invention provides a non-mouse pluripotential embryonic stem cell which can be maintained on feeder layers for at least 20 passages and give rise to embryoid bodies and multiple differentiated cell phenotypes in monolayer culture. The invention further provides a method of making a pluripotential embryonic stem cell comprising administering a growth enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to primordial germ cells under cell growth conditions, thereby making a pluripotential embryonic stem cell.
Inventors
- 👤 Brigid L. M. Hogan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 09:36
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0603; C12N5/0606; C12N2501/00; C12N2501/10; C12N2501/115; C12N2501/125; C12N2501/20; C12N2501/23; C12N2501/235; C12N2503/00; C12N2503/02; C12N2506/00; C12N2506/04; C12N2510/00
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
76 words
A method of screening factors for the ability to promote the formation of embryonic stem cells, comprising combining primordial germ cells with a factor selected from the group consisting of fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor with the factor to be screened and determining the formation of embryonic stem cells, the formation of embryonic stem cells indicating a factor capable of promoting the formation of embryonic stem cells.
Claim 2
Independent Claim
76 words
A method of screening factors for the ability to promote the formation of embryonic stem cells, comprising combining primordial germ cells with a factor selected from the group consisting of fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor with the factor to be screened and determining the formation of embryonic stem cells, the formation of embryonic stem cells indicating a factor capable of promoting the formation of embryonic stem cells.
References Cited (Backward Citations)
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2000-09-22 | 2001-03-24 | 2001-09-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-09-22 | 2005-03-24 | 2005-09-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-09-22 | 2009-03-24 | 2009-09-23 | $7700.00 | Window Closed / Lapsed |