Patent EP0710668A2 successfully refreshed from Google Patents.
EP0710668A2
Expired / Lapsed
EP
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Covalent attachment of thiazole orange to oligonucleotides for use in nucleic acid detection
Assignee: Becton Dickinson and Co
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Filing: 1995-07-18
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Granted: —
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Expires: 2015-07-18
Abstract
Abstract The present invention relates to conjugates of a cyanine dye and an oligonucleotide. When these conjugates hybridize or bind to a target, a detectable increase in fluorescence intensity or change in fluorescence polarization is observed.
Inventors
- 👤 C. Preston Linn
- 👤 J. Bruce Pitner
- 👤 Pat D. Mize
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 21:19
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
77 words
A compound of the formula wherein X is O, S, Se, N-alkyl having from 1-6 carbons or C(CH₃) n ; R₁ is alkyl having from 1-6 carbons; R₂ is alkyl having from 1-6 carbons; R₃ is fused benzene, alkyl having from 1-6 carbons, methoxy or is absent; R₄ is alkyl having from 1-6 carbons methoxy or is absent; Z is an oligonucleotide having from 8 to 50 bases; and n is zero or an integer from 1-6.
Claim 2
Dependent Claim
9 words
The compound of claim 1 wherein X is S.
Claim 3
Dependent Claim
9 words
The compound of claim 2 wherein n is 3.
Claim 4
Dependent Claim
9 words
The compound of claim 2 wherein n is 6.
Claim 5
Dependent Claim
9 words
The compound of claim 1 wherein X is O.
Claim 6
Dependent Claim
9 words
The compound of claim 5 wherein n is 3.
Claim 7
Dependent Claim
9 words
The compound of claim 5 wherein n is 6.
Claim 8
Dependent Claim
9 words
The compound of claim 1 wherein X is Se.
Claim 9
Dependent Claim
9 words
The compound of claim 8 wherein n is 3
Claim 10
Dependent Claim
9 words
The compound of claim 8 wherein n is 6.
References Cited (Backward Citations)
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