US4957870A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Detection of Reticulocytes, RNA and DNA
Assignee: Becton Dickinson and Co
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Filing: 1989-04-03
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Granted: 1990-09-18
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Expires: 2009-04-03
Abstract
Abstract Reticulocytes, RNA or DNA are stained with a dye for detection in a flow cytometer. The dye has the formula: ##STR1## Wherein X=O, S, se or C(CH 3 ) 2 ; R 1 =alkyl having from 1-6 carbons; R 2 =alkyl having from 1-6 carbons; R 3 =fused benzene, alkyl (having 1-6 carbons), methoxy or is absent; R 4 =alkyl having 1-6 carbons, methoxy or is absent; and n=zero or an integer from 1-6.
Inventors
- 👤 Linda G. Lee
- 👤 Chia-Huei Chen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 13:45
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/80; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
93 words
In a process for detecting reticulocytes, RNA or DNA in a sample, the improvement comprising: adding to said sample a fluorescent dye having the formula ##STR7## wherein: X=0, S, Se or C (CH 3 ) 2 ; R 1 =alkyl having from 1-6 carbons; R 2 =alkyl having from 1-6 carbons; R 3 =fused benzene, alkyl (having 1-6 carbons), methoxy or hydrogen; R 4 =alkyl having 1-6 carbons, methoxy or hydrogen; and n=zero or an integer from 1-6; exciting said sample with light of excitation wavelength; and measuring fluorescence emitted from said sample.
Claim 2
Dependent Claim
93 words
In a process for detecting reticulocytes, RNA or DNA in a sample, the improvement comprising: adding to said sample a fluorescent dye having the formula ##STR7## wherein: X=0, S, Se or C (CH 3 ) 2 ; R 1 =alkyl having from 1-6 carbons; R 2 =alkyl having from 1-6 carbons; R 3 =fused benzene, alkyl (having 1-6 carbons), methoxy or hydrogen; R 4 =alkyl having 1-6 carbons, methoxy or hydrogen; and n=zero or an integer from 1-6; exciting said sample with light of excitation wavelength; and measuring fluorescence emitted from said sample.
Claim 3
Dependent Claim
20 words
The process of claim 1 wherein the sample is excited and fluorescence is measured by means of a flow cytometer.
Claim 4
Dependent Claim
19 words
The process of claim 1 wherein the sample is excited and fluorescence is measured by means of fluorescence microscopy.
Claim 5
Dependent Claim
20 words
The process of claim 2 wherein the samples excited in the flow cytometer with light from a mercury arc lamp.
Claim 6
Dependent Claim
20 words
The process of claim 2 wherein the sample is excited in the flow cytometer with light from an argon laser.
Claim 7
Dependent Claim
11 words
The process of claim 2 wherein the sample comprises whole blood.
Claim 8
Dependent Claim
15 words
The process of claim 6 wherein the sample is not fixed with a cell fixative.
Claim 9
Dependent Claim
25 words
The process of claim 1 wherein R 1 ═CH 3 , R 2 ═CH 3 , R 3 ═hydrogen, R 4 ═hydrogen, X═S and n═1.
Claim 10
Dependent Claim
26 words
The process of claim 1 wherein R 1 ═CH 3 , R 2 ═CH 3 , R 3 ═fused benzene, R 4 ═hydrogen, X═S and n═0.
Claim 11
Dependent Claim
25 words
The process of claim 1 wherein R 1 ═CH 3 , R 2 ═CH 3 , R 3 ═hydrogen, R 4 ═hydrogen, X═0 and n═zero.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-09-17 | 1994-03-19 | 1994-09-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-09-17 | 1998-03-19 | 1998-09-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-09-17 | 2002-03-19 | 2002-09-18 | $7700.00 | Window Closed / Lapsed |