US4707451A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Detection of reticulocytes
Assignee: Becton Dickinson and Co
•
Filing: 1985-09-18
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Granted: 1987-11-17
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Expires: 2005-09-18
Abstract
Abstract Reticulocytes are stained with thioflavin T or chrysaniline for detection in a flow cytometer.
Inventors
- 👤 Burton H. Sage, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 03:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/80
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
86 words
A process for determining reticulocytes comprising: (a) staining reticulocytes in a blood sample with chrysaniline to give a mixture; (b) exciting chrysaniline-stained reticulocytes in said mixture with light energy from an output peak of a laser having a maximum output at about 488 nm; and (c) detecting said stained reticulocytes and distinguishing them from chrysaniline-stained erythrocytes in said mixture by detecting in a flow cytometer fluorescence emission from said stained reticulocytes at an emission wavelength different from the wavelength of fluorescence emission from said stained erythrocytes.
Claim 2
Dependent Claim
86 words
A process for determining reticulocytes comprising: (a) staining reticulocytes in a blood sample with chrysaniline to give a mixture; (b) exciting chrysaniline-stained reticulocytes in said mixture with light energy from an output peak of a laser having a maximum output at about 488 nm; and (c) detecting said stained reticulocytes and distinguishing them from chrysaniline-stained erythrocytes in said mixture by detecting in a flow cytometer fluorescence emission from said stained reticulocytes at an emission wavelength different from the wavelength of fluorescence emission from said stained erythrocytes.
Claim 3
Dependent Claim
23 words
The process of claim 1 wherein said chrysaniline stained reticulocytes are excited in said flow cytometer with light from an argon ion laser.
Claim 4
Dependent Claim
13 words
The process of claim 1 wherein said reticulocytes are detected without fixation thereof.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-16 | 1991-05-18 | 1991-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-16 | 1995-05-18 | 1995-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-16 | 1999-05-18 | 1999-11-17 | $7700.00 | Window Closed / Lapsed |