US4332785A
Expired / Lapsed
US
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Immunoassay for measurement of reticulocytes, and immunoreactive reagents for use therein
Assignee: University Patents Inc
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Filing: 1980-04-09
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Granted: 1982-06-01
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Expires: 2000-04-09
Abstract
Abstract The reticulocyte content present in a specimen of red blood cells is quantitatively measured based upon the selective immunoreactivity of the reticulocyte portion of the specimen with a reticulocyte-specific antibody which is immunoreactive with proteinaceous material associated with reticulocytes but not associated with mature red blood cells. Such immunoreactive proteinaceous material may be transferrin, transferrin receptor, transcobalamin II, or transcobalamin II receptor. Various procedures are described for quantitating such selective immunoreactivity, including fluorescent and radioactive detection techniques employing direct or indirect fluorescent or radioactive labeling of the reticulocyte-specific antibody.
Inventors
- 👤 Robert H. Allen
- 👤 Paul A. Seligman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 21:53
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/80; Y; Y10; Y10S; Y10S435/00; Y10S435/966; Y10S435/968; Y10S436/00; Y10S436/80; Y10S436/801; Y10S436/804
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
107 words
A method for the quantitative assay of the reticulocyte content present in a specimen of red blood cells, comprising the steps of: (a) forming an incubation mixture comprising said specimen and a reticulocyte-specific antibody which is immunoreactive with proteinaceous material substantially associated with reticulocytes but not substantially associated with mature red blood cells; (b) incubating said incubation mixture for a period of time sufficient to enable immunoreaction to occur, whereby said reticulocyte-specific antibody forms an immunoreaction product selectively with the reticulocyte portion of said specimen via said immunoreactive proteinaceous material; and (c) quantitatively measuring the reticulocyte portion of said specimen based upon the extent of said immunoreaction.
Claim 2
Independent Claim
107 words
A method for the quantitative assay of the reticulocyte content present in a specimen of red blood cells, comprising the steps of: (a) forming an incubation mixture comprising said specimen and a reticulocyte-specific antibody which is immunoreactive with proteinaceous material substantially associated with reticulocytes but not substantially associated with mature red blood cells; (b) incubating said incubation mixture for a period of time sufficient to enable immunoreaction to occur, whereby said reticulocyte-specific antibody forms an immunoreaction product selectively with the reticulocyte portion of said specimen via said immunoreactive proteinaceous material; and (c) quantitatively measuring the reticulocyte portion of said specimen based upon the extent of said immunoreaction.
Claim 3
Dependent Claim
28 words
The method of claim 1, wherein said immunoreactive proteinaceous material is selected from the group consisting of transferrin, transferrin receptor, transcobalamin II, transcobalamin II receptor, and combinations thereof.
Claim 4
Dependent Claim
13 words
The method of claim 2, wherein said immunoreactive proteinaceous material is transferrin receptor.
Claim 5
Dependent Claim
26 words
The method of claim 1, wherein the quantitative measurement of the reticulocyte portion of said spcimen is based upon the amount of said immunoreaction product formed.
Claim 6
Dependent Claim
27 words
The method of claim 4, wherein said reticulocyte-specific antibody is in fluorescent-labeled form, and the amount of said immunoreaction product formed is determined by fluorescent detection techniques.
Claim 7
Dependent Claim
27 words
The method of claim 4, wherein said reticulocyte-specific antibody is in radioactive-labeled form, and the amount of said immunoreaction product formed is determined by radioactive detection techniques.
Claim 8
Dependent Claim
44 words
The method of claim 4, including the further step subsequent to step (b) of incubating said immunoreaction product with a fluorescent-labeled substance which is bindable to the immunoreacted reticulocyte-specific antibody, and the amount of said immunoreaction product formed is determined by fluorescent detection techniques.
Claim 9
Dependent Claim
19 words
The method of claim 7, wherein said fluorescent-labeled substance is a fluorescent-labeled second antibody directed against said reticulocyte-specific antibody.
Claim 10
Dependent Claim
44 words
The method of claim 4, including the further step subsequent to step (b) of incubating said immunoreaction product with a radioactive-labeled substance which is bindable to the immunoreacted reticulocyte-specific antibody, and the amount of said immunoreaction product formed is determined by radioactive detection techniques.
Claim 11
Dependent Claim
19 words
The method of claim 9, wherein said radioactive-labeled substance is a radioactive-labeled second antibody directed against said reticulocyte-specific antibody.
Claim 12
Dependent Claim
34 words
The method of claim 4, wherein complement to said reticulocyte-specific antibody is added to said incubation mixture, and the amount of said immunoreaction product formed is determined by measurement of the resulting complement-mediated effects.
Claim 13
Dependent Claim
30 words
The method of claim 1, wherein the quantitative measurement of the reticulocyte portion of said specimen is based upon the amount of said immunoreactive proteinaceous material present in said specimen.
Claim 14
Dependent Claim
50 words
The method of claim 12, wherein said incubation mixture contains a known amount of said immunoreactive proteinaceous material in addition to that present in said specimen, said known amount being in radioactive-labeled form, and the amount of said immunoreactive proteinaceous material present in said specimen is determined by radioimmunoassay techniques.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-31 | 1985-11-30 | 1986-06-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-31 | 1989-11-30 | 1990-06-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-31 | 1993-11-30 | 1994-06-01 | $7700.00 | Window Closed / Lapsed |