Patent US2126800A successfully refreshed from Google Patents.
Patent EP0502504A1 successfully refreshed from Google Patents.
EP0502504A1
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EP
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A biosensor utilizing enzyme and a method for producing the same
Assignee: Panasonic Holdings Corp
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Filing: 1992-03-04
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Granted: —
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Expires: 2012-03-04
Abstract
Abstract The biosensor of the present application determines an analyte (eq fructose) contained in a sample liquid by reducing electron acceptors using electrons generated in a reaction of the analyte with an enzyme and then by measuring the reduced amount of the electron acceptors electrochemically. The biosensor has an electrical insulating substrate (1), an electrode system (14) including at least a working electrode (4) and a counter electrode (5), a reaction layer (15) including the enzyme (7) provided on the electrode system and a hydrogen ion concentration (pH) control layer (11), and the reaction layer is in contact with the electrode system. According to the present application, the pH of the sample can be optimised for the type of the enzyme contained in the reaction layer, without prior addition of pH buffer to the sample liquid. Thus, the specific analyte contained in the sample liquid can be easily determined with accuracy and speed.
Inventors
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 21:27
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
93 words
A biosensor for quantifying a substrate contained in a sample liquid by reducing electron acceptors using electrons generated in a reaction of the substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically, the biosensor comprising: an electrical insulating substrate, an electrode system including at least a working electrode and a counter electrode which are formed on the insulating substrate, a reaction layer including the enzyme provided on the electrode system, and a hydrogen ion concentration control layer; the reaction layer being in contact with the electrode system.
Claim 2
Dependent Claim
17 words
A biosensor according to claim 1, wherein the reaction layer further includes hydrophilic polymer and electron acceptors.
Claim 3
Dependent Claim
24 words
A biosensor according to claim 1, wherein the reaction layer further includes hydrophilic polymer and the hydrogen ion concentration control layer includes electron acceptors.
Claim 4
Dependent Claim
38 words
A biosensor according to claim 2, wherein the reaction layer is formed by laminating a first layer including hydrophilic polymer and enzyme, a second layer including hydrophilic polymer and a third layer including electron acceptors in this order.
Claim 5
Dependent Claim
19 words
A biosensor according to claim 1, wherein the hydrogen ion concentration control layer is provided on the electrode system.
Claim 6
Dependent Claim
35 words
A biosensor according to claim 5, wherein the reaction layer further includes hydrophilic polymer and electron acceptors, and a hydrophilic polymer layer is provided between the reaction layer and the hydrogen ion concentration control layer.
Claim 7
Dependent Claim
44 words
A biosensor according to claim 5, wherein the reaction layer comprises a first layer including hydrophilic polymer and electron acceptors and a second layer including enzyme laminated on the first layer, and the hydrogen ion concentration control layer is provided on the second layer.
Claim 8
Dependent Claim
15 words
A biosensor according to claim 1, wherein the electrode system is mainly formed from carbon.
Claim 9
Dependent Claim
43 words
A biosensor according to claim 1, wherein the hydrogen ion concentration control layer includes buffering salt selected from the group consisting of potassium biphosphate-dipotassium phosphate, potassium biphosphate-disodium phosphate, citric acid-disodium phosphate, citric acid-trisodium citrate, potassium bicitrate-sodium hydroxide and maleic acid monosodium salt-sodium hydroxide.
Claim 10
Dependent Claim
40 words
A biosensor according to claim 1, wherein the hydrophilic polymer is selected from the group consisting of carboximethyl cellulose, polyvinylpyrrolidone, hydroxyethyl cellulose, hydroxy proryl cellulose, methyl cellulose, ethyl cellulose, ethyl hydroxyethyl cellulose, carboxy methyl ethyl cellulose, polyvinyl alcohol and gelatin.
Claim 11
Dependent Claim
36 words
A biosensor according to claim 1, wherein the enzyme is selected from the group consisting of fructose dehydrogenase, invertase, mutarotase, glucose oxidase, alcohol oxidase, lactate oxidase, lactate dehydrogenase, cholesterol oxidase, xanthine oxidase and amino acid oxidase.
Claim 12
Dependent Claim
23 words
A biosensor according to claim 1, wherein the electron acceptor is selected from the group consisting of potassium ferricyanide, p-benzoquinone, phenazinemethosulfate and ferrocene.
Claim 13
Independent Claim
98 words
A method for producing a biosensor for quantifying a substrate contained in a sample liquid by reducing electron acceptors using electrons generated in a reaction of the substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically, the method comprising the steps of: firstly, providing an electrode system including at least a working electrode and a counter electrode on an electrical insulating substrate; secondly, forming a hydrogen ion concentration control layer including electron acceptors on the insulating substrate; and finally, forming a reaction layer including hydrophilic polymer and enzyme on the electrode system.
References Cited (Backward Citations)
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