EP0400918A1
Expired / Lapsed
EP
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Enzyme sensor
Assignee: Nakano Vinegar Co Ltd
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Filing: 1990-05-24
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Granted: —
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Expires: 2010-05-24
Abstract
Abstract An enzyme sensor comprising an enzyme-modified electrode and a counter electrode, wherein the enzyme-modified electrode comprises, on an electrode substrate, a homogenous composition comprising an electron-transfer mediator and an enzyme and/or an enzyme-containing substance. The enzyme sensor is useful in analysis, such as the analysis of compounds in foods or components in the living body, the diagnosis of diseases and the control of reaction processes. The preparation of the enzyme-modified electrode is also described.
Inventors
- 👤 Akira Nanba
- 👤 Masahiro Fukaya
- 👤 Hajime Okumura
- 👤 Yoshiya Kawamura
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 16:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817; Y10S436/00; Y10S436/806
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
43 words
An enzyme sensor which comprises an enzyme-modified electrode and a counter electrode, wherein the enzyme-modified electrode comprises an electrode substrate and a homogenous composition comprising an electron transfer mediator and an enzyme and/or enzyme containing substance on a surface of the electrode substrate.
Claim 2
Dependent Claim
43 words
An enzyme sensor which comprises an enzyme-modified electrode and a counter electrode, wherein the enzyme-modified electrode comprises an electrode substrate and a homogenous composition comprising an electron transfer mediator and an enzyme and/or enzyme containing substance on a surface of the electrode substrate.
Claim 3
Dependent Claim
39 words
A sensor according to claim 1, wherein the enzyme-containing substance is cells, a cultured medium comprising disrupted cells of an enzyme-producing microorganism, or a fractionation component, cellular extract, cell membrane fraction or crude enzyme from such disrupted micro-organism cells.
Claim 4
Dependent Claim
14 words
A sensor according to claim 1 or 2, wherein the enzyme is a dehydrogenase.
Claim 5
Dependent Claim
24 words
A sensor according to claim 3, wherein the dehydrogenase is an alcohol dehydrogenase, aldehyde dehydrogenase, glucose dehydrogenase, fructose dehydrogenase, sorbitol dehydrogenase or glycerol dehydrogenase.
Claim 6
Dependent Claim
23 words
A sensor according to any one of claims 1 to 4, wherein the electron transfer mediator is a redox compound and/or a coenzyme.
Claim 7
Dependent Claim
31 words
A sensor according to any one of claims 1 to 5, wherein the electron transfer mediator is p-benzoguinone, ferrocene, dimethylferrocene, potassium ferricyanide, phenazine methosulphate, 2,6-dichlorophenol indophenol, PQQ, FAD, NAb or NADP.
Claim 8
Dependent Claim
35 words
A sensor according to claim 6, wherein the enzyme and electron transfer mediator are, respectively: an aldehyde dehydrogenase and p-benzoguinone; fructose dehydrogenase and dimethylferrocene; sorbitol dehydrogenase and dimethyl ferrocene; or glycerol dehydrogenase and potassium ferricyanide.
Claim 9
Dependent Claim
17 words
A sensor according to any one of claims 1 to 7, wherein the homogenous composition comprises carbon.
Claim 10
Dependent Claim
15 words
A sensor according to claim 8 wherein the carbon is in the form of graphite.
Claim 11
Dependent Claim
13 words
An enzyme-modified electrode as defined in any one of claims 1 to 9.
Claim 12
Independent Claim
56 words
A method of preparing an enzyme modified-electrode which comprises preparing a composition by dissolving a water-insoluble electron-transfer mediator in an organic solvent, adding liquid paraffin thereto, removing the organic solvent and mixing the resultant mixture with graphite powder and an enzyme and/or an enzyme-containing substance, and applying the resulting composition to the surface of an electrode.
Claim 13
Independent Claim
56 words
A method of preparing an enzyme modified-electrode which comprises preparing a composition by dissolving a water-insoluble electron-transfer mediator in an organic solvent, adding liquid paraffin thereto, removing the organic solvent and mixing the resultant mixture with graphite powder and an enzyme and/or an enzyme-containing substance, and applying the resulting composition to the surface of an electrode.
Claim 14
Independent Claim
46 words
A method of preparing an enzyme-modified electrode which comprises preparing a composition by mixing reversed micelles, into which a water-soluble electron-transfer mediator is incorporated, with graphite powder and an enzyme and/or an enzyme-containing substance, and then applying the resulting composition to the surface of an electrode.
Claim 15
Independent Claim
46 words
A method of preparing an enzyme-modified electrode which comprises preparing a composition by mixing reversed micelles, into which a water-soluble electron-transfer mediator is incorporated, with graphite powder and an enzyme and/or an enzyme-containing substance, and then applying the resulting composition to the surface of an electrode.
Claim 16
Independent Claim
52 words
A method of preparing an enzyme-modified electrode which comprises preparing a composition by mixing a water-insoluble complex of ferricyanide and a cationic surface active agent with liquid paraffin and then with graphite powder and an enzyme and/or an enzyme-containing substance, and then applying the resulting composition to the surface of an electrode.
Claim 17
Independent Claim
52 words
A method of preparing an enzyme-modified electrode which comprises preparing a composition by mixing a water-insoluble complex of ferricyanide and a cationic surface active agent with liquid paraffin and then with graphite powder and an enzyme and/or an enzyme-containing substance, and then applying the resulting composition to the surface of an electrode.
Claim 18
Dependent Claim
79 words
An apparatus for analysing the components in a solution, which comprises a reaction chamber equipped with a sample injection port, an electrolyte inlet, a waste liquid-outlet, a stirring device, a counter electrode, and an enzyme-modified electrode as defined in any one of claims 1 to 9 or as produced by the method as claimed in claim 11, 15 or 13, and which further comprises a constant voltage power supply part, a current voltage converting part, and an amplifier part.
References Cited (Backward Citations)
EP0177743A2
Cited
WO1989010395A1
Cited
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