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EP0440304A1
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Fungal resistant plants, process for obtaining fungal resistant plants and recombinant polynucleotides for use therein
Assignee: Rijksuniversiteit Te Leiden syngenta Mogen Bv
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Filing: 1991-01-30
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Granted: —
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Expires: 2011-01-30
Abstract
Abstract Plants are provided with improved resistance against pathogenic fungi. They are genetically transformed with one or more polynucleotides which essentially comprise one or more genes encoding plant chitinases and β-1,3-glucanases. Preferred are the intracellular forms of the said hydrolytic enzymes, especially preferred are those forms which are targeted to the apoplastic space of the plant by virtue of the modification of the genes encoding the said enzymes. Particularly preferred are plants exhibiting a relative overexpression of at least one gene encoding a chitinase and one gene encoding a β-1,3-glucanase.
Inventors
- 👤 Bernardus Johannes Clemens Cornelissen
- 👤 Leo Sjoerd Melchers
- 👤 Elisabeth Josine Sophie Meulenhoff
- 👤 Jeroen Sebastiaan Charles Van Roekel
- 👤 Marianne Beatrix Sela-Buurlage
- 👤 Alexandra Aleida Vloemans
- 👤 Charles Peter Woloshuk
- 👤 John Ferdinand Bol
- 👤 Hubertus Josephus Maria Linthorst
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 05:30
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8279; C12N15/8282; A; A01; A01N; A01N65/00; A01N65/08; A01N65/38; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/244; C12N9/2442; C12Y; C12Y302/00; C12Y302/01; C12Y302/01006; C12Y302/01014; C12Y302/01039; C12Y302/01058
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
42 words
A plant which exhibits, as a result of genetic manipulation of the said plant or an ancestor thereof, through the use of one or more recombinant polynucleotides, a relative overexpression of an intracellular chitinase gene, in at least one of the tissues.
Claim 2
Dependent Claim
42 words
A plant which exhibits, as a result of genetic manipulation of the said plant or an ancestor thereof, through the use of one or more recombinant polynucleotides, a relative overexpression of an intracellular chitinase gene, in at least one of the tissues.
Claim 3
Dependent Claim
24 words
The plant according to Claim 1, in which the intracellular chitinase is targeted to the apoplast, due to modification of the gene encoding it.
Claim 4
Dependent Claim
32 words
The plant according to Claim 2, in which the gene encoding said intracellular chitinase gene is modified by creating a translation stopcodon in the coding region at the 3'-end of the gene.
Claim 5
Dependent Claim
25 words
The plant according to Claim 3, in which the creation of the translation stopcodon results in deletion of between 3 and 10 C-terminal amino acids.
Claim 6
Dependent Claim
55 words
A plant which exhibits, as a result of the genetic manipulation of the said plant, or an ancestor, or any plant part regenerated to produce the said plant, through the use of one or more recombinant polynucleotides, a relative overexpression of a chitinase gene and a 13-1,3-glucanase gene in at least one of the tissues.
Claim 7
Dependent Claim
55 words
A plant which exhibits, as a result of the genetic manipulation of the said plant, or an ancestor, or any plant part regenerated to produce the said plant, through the use of one or more recombinant polynucleotides, a relative overexpression of a chitinase gene and a 13-1,3-glucanase gene in at least one of the tissues.
Claim 8
Dependent Claim
38 words
The plant according to any of the Claims 1 to 4, which further exhibits the relative overexpression of at least one gene encoding an enzyme selected from the group consisting of extracellular chitinases, intracellular 13-1,3-glucanases, and extracellular β-1,3-glucanases.
Claim 9
Dependent Claim
27 words
The plant according to any of the Claims 1-4, which further exhibits the relative overexpression of at least an extracellular chitinase gene and an intracellular β-1,3-glucanase gene.
Claim 10
Dependent Claim
29 words
The plant according to any of the Claims 1-4, which further exhibits the relative overexpression of an extracellular chitinase gene, an intracellular β-1,3-glucanase gene and an extracellular 13-1,3-glucanase gene.
Claim 11
Dependent Claim
29 words
The plant according to any of the Claims 6 to 8, in which the intracellular β-1,3-glucanase is targeted to the apoplast, due to modification of the gene encoding it.
Claim 12
Dependent Claim
32 words
The plant according to Claim 9, in which the gene encoding the intracellular 13-1,3-glucanase gene is modified by creating a translation stopcodon in the coding region at the 3'-end of the gene.
Claim 13
Dependent Claim
29 words
The plant according to Claim 10, in which the creation of the translation stopcodon results in deletion of between 3 and 25 C-terminal amino acids of the intracellular β-1,3-glucanase.
Claim 14
Dependent Claim
26 words
The plant according to any of the Claims 1 to 11, in which the newly introduced genes are under the control of the CaMV 35S promoter.
Claim 15
Dependent Claim
67 words
A recombinant polynucleotide, comprising genetic information for the relative overexpression of an intracellular chitinase gene, essentially comprising, a) a promoter that is functional in plants, b) a gene encoding the intracellular chitinase, under the control of the said promoter, and c) a terminator operably linked to the said gene, and d) a gene encoding a selectable or screenable trait, operably linked to regulatory sequences for proper expression.
Claim 16
Dependent Claim
67 words
A recombinant polynucleotide, comprising genetic information for the relative overexpression of an intracellular chitinase gene, essentially comprising, a) a promoter that is functional in plants, b) a gene encoding the intracellular chitinase, under the control of the said promoter, and c) a terminator operably linked to the said gene, and d) a gene encoding a selectable or screenable trait, operably linked to regulatory sequences for proper expression.
Claim 17
Dependent Claim
32 words
A recombinant polynucleotide according to Claim 13, in which the gene encoding the intracellular chitinase is modified by creating a translation stopcodon in the coding region at the 3'-end of the gene.
Claim 18
Dependent Claim
26 words
A recombinant polynucleotide according to Claim 14, in which the stopcodon results in deletion of between 3 and 10 C-terminal amino acids of the intracellular chitinase.
Claim 19
Dependent Claim
82 words
A recombinant polynucleotide according to any of the Claims 13 to 15, additionally comprising the genetic information for the relative overexpression of at least one gene encoding an enzyme selected from the group consisting of extracellular chitinases, intracellular β-1,3-glucanases and extracellular β-1,3-glucanases, said genetic information essentially comprising, e) a promoter that is functional in plants, f) the said gene encoding one of the said enzymes, under the control of the said promoter, and g) a terminator, operably linked to the said gene.
Claim 20
Dependent Claim
33 words
A recombinant polynucleotide according to Claim 16, additionally comprising the genetic information for the relative overexpression of a gene encoding an extracellular chitinase, in which f) is a gene encoding an extracellular chitinase.
Claim 21
Dependent Claim
33 words
A recombinant polynucleotide according to Claim 16, additionally comprising the genetic information for the relative overexpression of a gene encoding an intracellular /3-1,3-glucanase, in which f) is a gene encoding an intracellular 13-1,3-glucanase.
Claim 22
Dependent Claim
33 words
A recombinant polynucleotide according to Claim 16, additionally comprising the genetic information for the relative overexpression of a gene encoding an extracellular β-1,3-glucanase, in which f) is a gene encoding an extracellular 13-1,3-glucanase.
Claim 23
Dependent Claim
67 words
A recombinant polynucleotide, comprising the genetic information for the relative overexpression of an intracellular β-1,3-glucanase gene, essentially comprising, a) a promoter that is functional in plants, b) a gene encoding the intracellular β-1,3-glucanase, under the control of the said promoter, c) a terminator, operably linked to the said gene, and d) a gene encoding a selectable or screenable trait, operably linked to regulatory sequences for proper expression.
Claim 24
Dependent Claim
67 words
A recombinant polynucleotide, comprising the genetic information for the relative overexpression of an intracellular β-1,3-glucanase gene, essentially comprising, a) a promoter that is functional in plants, b) a gene encoding the intracellular β-1,3-glucanase, under the control of the said promoter, c) a terminator, operably linked to the said gene, and d) a gene encoding a selectable or screenable trait, operably linked to regulatory sequences for proper expression.
Claim 25
Dependent Claim
35 words
A recombinant polynucleotide, according to Claim 16, 18, or 20, in which the gene encoding the intracellular β-1,3-glucanase is modified by creating a translation stopcodon in the coding region at the 3'-end of the gene.
Claim 26
Dependent Claim
26 words
A recombinant polynucleotide according to Claim 21, in which the stopcodon results in deletion of between 3 and 25 C-terminal amino acids of the intracellular 13-1,3-glucanase.
Claim 27
Dependent Claim
65 words
A recombinant polynucleotide according to any of the Claims 21 or 22, additionally comprising the genetic information for the relative overexpression of at least a gene encoding an extracellular β-1,3- glucanase, essentially comprising, e) a promoter that is functional in plants, f) a gene encoding the extracellular β-1,3-glucanase, under the control of the said promoter, and g) a terminator, operably linked to the said gene.
Claim 28
Dependent Claim
17 words
A cloning or transformation vector comprising the recombinant polynucleotides of any of the Claims 13 to 23.
Claim 29
Dependent Claim
18 words
A plasmid selected from the group consisting of pMOG200, pMOG212, pMOG289 and pMOG512, as well as derivatives thereof.
Claim 30
Dependent Claim
18 words
A plasmid selected from the group consisting of pMOG200, pMOG212, pMOG289 and pMOG512, as well as derivatives thereof.
Claim 31
Dependent Claim
10 words
Bacteria harboring at least a plasmid according to Claim 25.
Claim 32
Dependent Claim
44 words
A process for obtaining fungal resistant plants by introducing into the genome of the said plants, or their ancestors, or any plant part that can be regenerated to produce the said plant, a recombinant polynucleotide of any one of the Claims 13 to 23.
References Cited (Backward Citations)
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EP0292435A1
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EP0332104A2
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WO1990007001A1
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