EP0270248A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
DNA useful for the transport of foreign proteins to the plant cell wall
Assignee: Syngenta Ltd
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Filing: 1987-11-03
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Granted: —
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Expires: 2007-11-03
Abstract
Abstract Novel DNA base sequences which comprise a first base sequence coding for a plant cell wall targeting protein fragment upstream of a second base sequence coding for a protein not naturally found in plant cell walls. Exemplified with reference to the polygalacturonase polypeptide leader and the β-glucuronidase (gus) gene.
Inventors
- 👤 Ian George Bridges
- 👤 Wolfgang Walter Schuch
- 👤 Donald Grierson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 05:52
CPC Classifications
C; C12; C12Y; C12Y302/00; C12Y302/01; C12Y302/01031; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8221; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12Y302/01015; C07; C07K; C07K2319/00; C07K2319/01; C07K2319/02
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
54 words
Recombinant DNA comprising a reading frame initiator commencing an upstream base sequence followed by a downstream base sequence in register therewith, the upstream sequence coding for at least a substantial part of a plant cell wall targeting protein fragment, and the downstream sequence coding for a protein not naturally found in plant cell walls.
Claim 2
Dependent Claim
54 words
Recombinant DNA comprising a reading frame initiator commencing an upstream base sequence followed by a downstream base sequence in register therewith, the upstream sequence coding for at least a substantial part of a plant cell wall targeting protein fragment, and the downstream sequence coding for a protein not naturally found in plant cell walls.
Claim 3
Dependent Claim
22 words
DNA as claimed in claim 1 in which the plant cell wall targeting protein fragment is the leader protein associated with prepolygalacturonase.
Claim 4
Dependent Claim
31 words
DNA as claimed in either of claims 1 or 2 in which the targeting protein fragment has the 71 amino-acid sequence shown in Figure 1 from base 51 to base 263.
Claim 5
Dependent Claim
23 words
DNA as claimed in any of claims 1 to 3 in which the upstream sequence codes for substantially all the targeting protein fragment.
Claim 6
Dependent Claim
33 words
DNA as claimed in any of claims 1 to 4 in which the upstream base sequence includes a substantial part of the sequence of bases numbered from 51 to 263 in Figure 1.
Claim 7
Dependent Claim
30 words
DNA as claimed in claims 4 and 5 having an upstream base sequence continuing beyond base 263 and terminating at the RsaI restriction site at bases 282-285 of Figure 1.
Claim 8
Dependent Claim
23 words
DNA as claimed in claim 5 having an upstream base sequence terminating at the HindIII restriction site at bases 195-201 of Figure 1.
Claim 9
Dependent Claim
20 words
DNA as claimed in any of claims 1 to 7 in which the downstream sequence codes for the protein β-glucuronidase.
Claim 10
Dependent Claim
20 words
DNA as claimed in any of claims 1-7 in which the downstream sequence codes for an insecticidal or antifungal protein.
Claim 11
Dependent Claim
18 words
DNA as claimed in claim 9 in which the protein is a Bacillus thuringiensis toxin, chitinase or β-glucanase.
Claim 12
Dependent Claim
13 words
A vector containing DNA as claimed in any of claims 1 to 10.
Claim 13
Dependent Claim
11 words
A vector as claimed in claim 11 which is a plasmid.
Claim 14
Dependent Claim
14 words
A vector as claimed in claim 12 which is derived from a pTAK plasmid.
Claim 15
Dependent Claim
56 words
A process for making a vector claimed in any of claims 11 to 13 which comprises cutting with a suitable restriction enzyme the upstream base sequence from a first vector containing it and cloning it into a second vector containing the downstream sequence, adjacent to, upstream of, and in reading frame register with said downstream sequence.
Claim 16
Dependent Claim
55 words
A process for making a vector claimed in any of claims 11 to 13 which comprises cutting with a suitable restriction enzyme the downstream base sequence from a vector containing it, and cloning it into a second vector containing the upstream sequence, adjacent to, downstream of, and in reading frame register with said upstream sequence.
Claim 17
Dependent Claim
17 words
A plant cell containing DNA claimed in any of claims 1 to 10 incorporated into its genome.
Claim 18
Dependent Claim
13 words
A cell as claimed in claim 16 which is of a dicotyledonous plant.
Claim 19
Dependent Claim
13 words
A cell as claimed in claim 17 in which the plant is tobacco.
Claim 20
Dependent Claim
11 words
Plants and seeds comprising cells claimed in any of claims 16-18.
References Cited (Backward Citations)
No citation records indexed.
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