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EP0284232A1 Expired / Lapsed EP ⏱️ 0.0 years remaining

Solid phase assay

Assignee: Becton Dickinson and Co
•
Filing: 1988-03-07
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Granted: —
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Expires: 2008-03-07
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Abstract

Abstract An article for use in an assay for an analyte, comprising a solid support having at least a first portion and a second portion, the first and second portions being in capillary flow communication with each other whereby material may flow by capillarity from the first portion to the second portion and the second portion containing a binder immobilized wherein the binder being a binder for at least the analyte; a tracer comprised of a ligand portion and a detectable label portion conjugated to the ligand portion, the tracer being supported on the solid support on the first portion thereof, whereby when the first portion is wetted with a liquid sample suspected of containing analyte, tracer and any analyte flow by capillarity to the second portion for contact with the binder. A method of assay for an analyte comprises contact­ing a sample suspected of containing analyte to be assayed with the first portion of the above article; and determining at least one of tracer which is bound or not bound in the second portion.

Inventors

  • 👤 Robert W. Rosenstein

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-02 00:30
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; G01N30/00; G01N30/90; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S435/97; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/829

Claims Analysis

Total Claims: 10
Claim 1
Independent Claim 131 words
An article for use in an assay for an analyte, comprising: a solid support having at least a first portion and a second portion, the first and second portions being in capillary flow communication with each other whereby material may flow by capillarity from the first portion to the second portion and the second portion containing a binder immobilized therein, the binder being a binder for at least the analyte; a tracer comprised of a ligand portion and a detectable label portion conjugated to the ligand portion, the tracer being supported on the solid support on the first portion thereof, whereby when the first portion is wetted with a liquid sample suspected of containing analyte, tracer and any analyte flow by capillarity to the second portion for contact with the binder.
Claim 2
Dependent Claim 131 words
An article for use in an assay for an analyte, comprising: a solid support having at least a first portion and a second portion, the first and second portions being in capillary flow communication with each other whereby material may flow by capillarity from the first portion to the second portion and the second portion containing a binder immobilized therein, the binder being a binder for at least the analyte; a tracer comprised of a ligand portion and a detectable label portion conjugated to the ligand portion, the tracer being supported on the solid support on the first portion thereof, whereby when the first portion is wetted with a liquid sample suspected of containing analyte, tracer and any analyte flow by capillarity to the second portion for contact with the binder.
Claim 3
Dependent Claim 26 words
An article as claimed in claim 1 in which the solid support is a strip and the first and secoond portions are in the same plane.
Claim 4
Dependent Claim 17 words
An article as claimed in claim 2 in which the second portion is comprised of a nitrocellulose.
Claim 5
Dependent Claim 25 words
An article as claimed in claim 2 in which the ligand portion of the tracer is bound by the binder immobilized on the second portion.
Claim 6
Dependent Claim 26 words
An article as claimed in any one of the preceding claims in which the tracer comprises a ligand conjugated to a sac having a detectable marker.
Claim 7
Dependent Claim 15 words
An article as claimed in claim 5 in which the sac includes a visible maker.
Claim 8
Dependent Claim 14 words
An article as claimed in claim 6 in which the sac is a liposome.
Claim 9
Dependent Claim 42 words
An article as claimed in any one of the preceding claims in which the solid support includes a third portion in capillary flow communication with the second portion whereby material may flow by capillarity from the second portion to the third portion.
Claim 10
Independent Claim 49 words
A method of assay for an analyte, comprising: contacting a sample suspected of containing the analyte to be assayed with the first portion of the article as claimed in any one of claims 1 - 9; and determining whether tracer is bound or not bound in the second portion.

References Cited (Backward Citations)

EP0154749A1 Cited
EP0212599A2 Cited
EP0225054A1 Cited
EP0255342A1 Cited

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