EP0154749A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Solid phase immunoassay with visual readout
Assignee: Becton Dickinson and Co
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Filing: 1984-12-14
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Granted: —
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Expires: 2004-12-14
Abstract
Abstract Solid phase assay for an analyte wherein binder is supported on a solid support, such as nitrocellulose, and the tracer is comprised of ligand labeled with a coloured particulate label, such as a liposome including a dye. The assay has a high sensitivity, and the tracer is visible on the support under assay conditions, whereby tracer can be determined, without instrumentation, and without further treatment thereof.
Inventors
- 👤 Robert L. Campbell
- 👤 Daniel B. Wagner
- 👤 James P. O'connell
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 06:42
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; B; B82; B82Y; B82Y5/00; G01N33/531; G01N33/532; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/807; Y10S436/808; Y10S436/809; Y10S436/815; Y10S436/817; Y10S436/818; Y10S436/829
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
129 words
A process of assaying for an analyte, comprising: contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being for at least the analyte, said tracer being comprised of a ligand labeled with a visible particulate label, said ligand being bound to one of the binder and analyte whereby the tracer is bound to one of the binder on the support and the analyte bound to the binder on the support, said test area being formed of a material having a surface area for supporting the binder in a concentration whereby bound tracer is visible on the support under assay conditions; and determining the visibility of tracer bound in said test area as a measure of analyte in a sample.
Claim 2
Dependent Claim
129 words
A process of assaying for an analyte, comprising: contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being for at least the analyte, said tracer being comprised of a ligand labeled with a visible particulate label, said ligand being bound to one of the binder and analyte whereby the tracer is bound to one of the binder on the support and the analyte bound to the binder on the support, said test area being formed of a material having a surface area for supporting the binder in a concentration whereby bound tracer is visible on the support under assay conditions; and determining the visibility of tracer bound in said test area as a measure of analyte in a sample.
Claim 3
Dependent Claim
19 words
The process of Claim 1 wherein analyte and tracer are sequentially contacted with the binder on the solid support.
Claim 4
Dependent Claim
19 words
The process of Claim 1 wherein the analyte and tracer are simultaneously contacted with binder on the solid support.
Claim 5
Dependent Claim
25 words
The process of any one of Claims, 1. 2 or 3 wherein the label of the tracer is a sac including a visible dye therein.
Claim 6
Dependent Claim
11 words
The process of Claim 4 wherein the sac is a liposome.
Claim 7
Dependent Claim
19 words
The process of Claim 5 wherein the visibility of the bound tracer is determined without lysis of the liposome.
Claim 8
Dependent Claim
17 words
The process of any one of the preceding claims wherein the solid support is in sheet form.
Claim 9
Dependent Claim
15 words
The process of any one of the preceding claims wherein the binder is an antibody.
Claim 10
Dependent Claim
19 words
The process of any one of the preceding claims wherein the tracer is a labeled form of the analyte.
Claim 11
Dependent Claim
21 words
The process of any one of Claims 1-8 wherein the tracer is a labeled form of an antibody for the analyte.
Claim 12
Dependent Claim
13 words
The process of any one of Claims 1-8 wherein the analyte is digoxin.
Claim 13
Dependent Claim
13 words
The process of any one of Claims 1-8 wherein the analyte is hCG
Claim 14
Dependent Claim
16 words
The process of any one of Claim 1-3 wherein the particulate label is a coloured particle.
Claim 15
Dependent Claim
40 words
The process of any one of the preceding claims wherein the test area is formed of nitrocellulose and the binder is supported in the test area in a concentration whereby bound tracer is visible on the support under assay conditions.
Claim 16
Dependent Claim
25 words
The process of any one of preceding claims wherein the analyte is in a sample in a concentration of no greater than 10 -9 gm/ml.
Claim 17
Dependent Claim
19 words
The process of any one of the preceding claims wherein the support contains a plurality of said test areas.
Claim 18
Dependent Claim
24 words
The process of any one of the preceding claims wherein the binder is supported in a concentration of at least 40 µg/cm 2 .
Claim 19
Dependent Claim
29 words
The process of any one of the preceding claims wherein the binder is in the test areas as a spot having a diameter of from 3 to 5 mm.
Claim 20
Dependent Claim
18 words
The process of any one of the preceding claims wherein the particulate label is a liposome containing rhodamine.
Claim 21
Dependent Claim
143 words
A reagent kit for determining an analyte in accordance with the process of any one of the preceding claims comprising: a solid support including a binder on a test area of a solid support; and a tracer, said binder being a binder for at least the analyte, said tracer being comprised of a ligand labeled with a visible particulate label, said ligand being bound to one of the binder on the support and the analyte bound to the binder on the support during an assay, said test area being formed of a material having a surface area for supporting the binder in a concentration whereby bound tracer is visible on the support under assay conditions, said solid support, binder, tracer and test area being the binder, tracer and test area employed in accordance with the process of any one of the preceding claims.
References Cited (Backward Citations)
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