EP0255342A1
Expired / Lapsed
EP
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Method of detecting or estimating biological materiel
Assignee: GD Searle LLC
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Filing: 1987-07-28
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Granted: —
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Expires: 2007-07-28
Abstract
Abstract Monoclonal antibodies raised against cell walls of Group A Streptococci are specific to biological materials, e.g. immunoglobulins, having terminal N-acetyl glucosamine residues and can be used in their detection, e.g. in the diagnosis of diseases characterized by their presence, e.g. rheumatoid arthritis and Crohn's disease.
Inventors
- 👤 Graham Arthur William Rook
- 👤 Jennifer Jane Edge
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 11:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/564; C; C07; C07K; C07K16/00; C07K16/12; C07K16/1267; C07K16/1275
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
37 words
A monoclonal antibody specific to biological materials containing terminal N-acetyl glucosamine residues, which is positive to bovine serum albumin conjugated to N-acetyl-glucosamine and to fetuin which has been treated with sialidase and galactosidase and negative to fetuin.
Claim 2
Dependent Claim
37 words
A monoclonal antibody specific to biological materials containing terminal N-acetyl glucosamine residues, which is positive to bovine serum albumin conjugated to N-acetyl-glucosamine and to fetuin which has been treated with sialidase and galactosidase and negative to fetuin.
Claim 3
Dependent Claim
18 words
A monoclonal antibody according to claim 1 raised against glycoprotein of Group A Streptococci having terminal N-acetyl-glucosamine residues.
Claim 4
Dependent Claim
16 words
A murine hybridoma capable of producing a monoclonal antibody as defined in claim 1 or 2.
Claim 5
Dependent Claim
32 words
A murine hybridoma according to claim 3 resulting from the fusion of a murine myeloma cell with an antibody-producing mouse spleen cell from a mouse immunized with Group A Streptococci cell walls.
Claim 6
Dependent Claim
62 words
A method of detecting or estimating a biological material having terminal N-acetyl-glucosamine residues which comprises causing the said material to bind to a monoclonal antibody as claimed in claim 1 or 2 and to a label under conditions such that the bound or unbound label provides a measure of the said biological material, and detecting or estimating said bound or unbound label.
Claim 7
Dependent Claim
55 words
A method according to claim 5 in which the biological material having terminal N-acetyl-glucosamine residues is bound to a solid support, the said monoclonal antibody is allowed to bind to the said material on the solid support, a label is attached directly or indirectly to said monoclonal antibody, and the said label is then assayed.
Claim 8
Dependent Claim
19 words
A method according to claim 6 in which the biological material is an immunoglobulin or a cell wall protein.
Claim 9
Dependent Claim
37 words
A method according to claim 6 in which the biological material is an immunoglobulin which is bound to the solid support by reaction with Protein A or Protein G which is itself bound to the said support.
Claim 10
Dependent Claim
56 words
A method according to claim 5 in which the biological material is a mammalian cell, the said monoclonal antibody is allowed to bind to any terminal N-acetyl-glucosamine residues on the surface of the said cell, a label is attached directly or indirectly to any bound monoclonal antibody, and the said label is then observed or assayed.
Claim 11
Independent Claim
30 words
A method according to any one of claims 5 to 9 in which the said label is an enzyme, which is assayed by a chromogenic reaction catalysed by said enzyme.
Claim 12
Independent Claim
31 words
A method according to any one of claims 5 to 10 in which the label is attached to the monoclonal antibody by an anti-mouse immunoglobulin covalently linked to the said label.
Claim 13
Independent Claim
40 words
A method according to any one of claims 5 to 10 in which the label is attached to the monoclonal antibody via biotin residues covalently linked to the said monoclonal antibody and avidin residues covalently linked to the said label.
Claim 14
Dependent Claim
37 words
A test kit useful in the method of any of claims 5 to 12 comprising a monoclonal antibody as claimed in claim 1 or 2, and a labelling reagent capable of binding to the said monoclonal antibody.
Claim 15
Dependent Claim
29 words
A test kit as claimed in claim 13 in which the labelling reagent includes an enzyme and the kit includes a colour-forming reagent for detecting or estimating said enzyme.
Claim 16
Dependent Claim
22 words
A test kit according to claim 14 in which the labelling reagent is an anti-mouse immunoglobulin covalently linked to the said enzyme.
Claim 17
Dependent Claim
26 words
A test kit according to claim 14 in which the monoclonal antibody is biotinylated and the labelling reagent comprises avidin covalently linked to the said enzyme.
Claim 18
Dependent Claim
30 words
A test kit according to any one of claims 13 to 16 which also comprises a solid support capable of binding to the biological material to be detected or estimated.
Claim 19
Dependent Claim
24 words
A test kit according to claim 17 in which the solid support is of nitrocellulose or other polymer capable of binding the biological material.
Claim 20
Dependent Claim
25 words
Use of a monoclonal antibody as claimed in claim 1 or 2 in the detection or estimation of a biological material containing terminal N-acetyl-glucosamine residues.
References Cited (Backward Citations)
GB1420806A
Cited
WO1984000758A1
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WO1984004169A1
Cited
EP0131415A2
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EP0150567A2
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EP0153477A1
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