Patent DE751236C successfully refreshed from Google Patents.
Patent EP0164206A1 successfully refreshed from Google Patents.
EP0164206A1
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An apparatus for the chemical synthesis of oligonucleotides
Assignee: Hamill Brendan James
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Filing: 1985-04-25
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Granted: —
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Expires: 2005-04-25
Abstract
Abstract © Apparatus for carrying out a multiplicity of different sequential chemical syntheses on solid-supports simultaneoulsy comprises a multiplicity of complementary support plates (15-26) each formed with a reaction chamber (39) opening through the plate and containing the solid-support (42) together with at least one by-pass channel (35-38) through the plate. The by-pass channel or channels (35-38) and reaction chamber (39) are disposed equi-angularly and at the same radial distance about an axis (33) of the plate (15). The plates (15-26) are rotatably supported in compression in face to face contact between end plates (13, 14) complementary to the plates (15-26) and each formed with a blank position and passages for at least one reactant stream. Means is provided for independently rotating each plate whereby each reaction chamber (39) of each plate (15-26) may independently be isolated or positioned in the or a selected reactant stream.
Inventors
- 👤 Brendan James Hamill
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 14:53
CPC Classifications
C; C07; C07H; C07H21/00; B; B01; B01J; B01J19/00; B01J19/0046; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00324; B01J2219/00326; B01J2219/00351; B01J2219/00423; B01J2219/00479; B01J2219/00488; B01J2219/00497; B01J2219/005; B01J2219/0054; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/0068; B01J2219/00686; B01J2219/00689; B01J2219/00718; B01J2219/0072; B01J2219/00722; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B60/00; C40B60/14; C40B70/00
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
143 words
Apparatus for carrying out a multiplicity of different sequential chemical syntheses on solid-supports simultaneously comprising a multiplicity of complementary support plates (15-26) each formed with a reaction chamber (39) opening through the plate and containing the solid-support (42) together with at least one by-pass channel (35-38) through the plate, said by-pass channel or channels (35-38) and reaction chamber (39) being disposed equi-angularly and at the same radial distance about an axis (33) of the plate (15), said plates (15-26) being rotatably supported in compression in face to face contact between end plates (13, 14) complementary to the plates (15-26) and each formed with a blank position and passages for at least one reactant stream, and means for independently rotating each plate whereby each reaction chamber (39) of each plate (15-26) may independently be isolated or positioned in the or a selected reactant stream.
Claim 2
Dependent Claim
143 words
Apparatus for carrying out a multiplicity of different sequential chemical syntheses on solid-supports simultaneously comprising a multiplicity of complementary support plates (15-26) each formed with a reaction chamber (39) opening through the plate and containing the solid-support (42) together with at least one by-pass channel (35-38) through the plate, said by-pass channel or channels (35-38) and reaction chamber (39) being disposed equi-angularly and at the same radial distance about an axis (33) of the plate (15), said plates (15-26) being rotatably supported in compression in face to face contact between end plates (13, 14) complementary to the plates (15-26) and each formed with a blank position and passages for at least one reactant stream, and means for independently rotating each plate whereby each reaction chamber (39) of each plate (15-26) may independently be isolated or positioned in the or a selected reactant stream.
Claim 3
Dependent Claim
20 words
Apparatus according to Claim 1, wherein there are four by-pass channels (35-38) in each plate (15) and four reactant streams.
Claim 4
Dependent Claim
14 words
Apparatus according to Claim 1 or 2, wherein the plates (15-26) are of polytetrafluoroethylene.
Claim 5
Dependent Claim
34 words
Apparatus according to Claim 1, 2 or 3, wherein the reaction chamber (39) in each plate (15-26) contains a pair of spaced porous plates (41, 43) between which the solid support (42) is held.
Claim 6
Dependent Claim
28 words
Apparatus according to any preceding claim, wherein the plates (15-26) are formed with central through-holes (33) through which a pin passes to provide rotatable support for said plates.
Claim 7
Dependent Claim
26 words
Apparatus according to any preceding claim, wherein there are also present dummy plates formed with at least five symmetrically positioned through holes and no reaction chamber.
Claim 8
Dependent Claim
93 words
Apparatus according to any preceding claim, further comprising a plate rotation head (65, 75) operable to engage a selected plate (15-26) to effect rotation thereof, means (64, 67) for indexing the rotation head (65, 75) stepwise along the plates (15-26) and a locator pin (63) engageable in one of a set of locator holes in each plate and indexed stepwise so that when the rotation head is engaging the mth plate in a stack of n plates (1<m<n) the newly aligned plates from 1 to (m-1) are engaged with the locator pin (63).
Claim 9
Dependent Claim
29 words
Apparatus according to Claim 7, wherein the locator pin (63) and plate rotation head (65) are driven by worms rotated by a common drive motor and gear box (67).
Claim 10
Dependent Claim
24 words
Apparatus according to Claim 7 or 8, wherein a double-acting ram engages the locator head with and withdraws it from engagement with each plate.
Claim 11
Dependent Claim
32 words
Apparatus according to Claims 7 to 9, wherein the edges of the plates are provided with optical markings and the plate rotation head includes a sensor that senses passage of the markings.
Claim 12
Dependent Claim
29 words
Apparatus according to Claims 7 to 10, including a ram (71) operable to apply pressure to the plates during passage of reactants and to release said pressure during indexing.
Claim 13
Dependent Claim
30 words
Apparatus according to Claim 11, wherein the ram (71) includes a position sensor operable to detect an extended position thereof from which the number of plates present can be detected.
Claim 14
Independent Claim
69 words
A method for carrying out a multiplicity of different sequential chemical syntheses simultaneously which comprises providing apparatus as claimed in Claims 1-12 and 16, and for each step adjusting the positions of the plates according to the compounds to be synthesised, applying pressure to the plates, passing reactant streams through the plates and releasing said pressure to permit the plate positions to be adjusted in the next following step.
Claim 15
Dependent Claim
14 words
A method according to Claim 13, wherein the compounds to be synthesised are oligonucleotides.
Claim 16
Independent Claim
119 words
An apparatus which comprises a series of plates clamped together, each of which plates may be moved independently of the others, and each of which plates contains a reaction chamber equipped with an inlet and outlet for the passage of fluids, and each of which plates also contains a number of fluid passages, the fluid passages and reaction chamber in each plate being constructed in such a way as to allow,when all the plates are suitably aligned, passage of at least four different fluid streams through all the plates of the apparatus, irrespective of the orientation of the reaction chamber in any individual plate relative to the orientation of the reaction chamber in any other plate in the apparatus.
Claim 17
Independent Claim
119 words
An apparatus which comprises a series of plates clamped together, each of which plates may be moved independently of the others, and each of which plates contains a reaction chamber equipped with an inlet and outlet for the passage of fluids, and each of which plates also contains a number of fluid passages, the fluid passages and reaction chamber in each plate being constructed in such a way as to allow,when all the plates are suitably aligned, passage of at least four different fluid streams through all the plates of the apparatus, irrespective of the orientation of the reaction chamber in any individual plate relative to the orientation of the reaction chamber in any other plate in the apparatus.
References Cited (Backward Citations)
EP0056721A2
Cited
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