Patent US1724085A successfully refreshed from Google Patents.
EP0056721A2
Expired / Lapsed
EP
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Chemical synthesis apparatus for preparation of polynucleotides
Assignee: Ens Bio Logicals Inc
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Filing: 1982-01-18
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Granted: —
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Expires: 2002-01-18
Abstract
Abstract n apparatus for the stepwise synthesis of polynucleotides in which the nucleotide chains are extended in stepwise fashion from a solid polymer support to which the first unit is linked comprises a reaction column containing the polymer supported product and acting as the reaction vessel, and a series of reagent bottles all connected to the reaction column by means of a fluid flow conduit to which the vessels make connection via two-way valves arranged in series. The farthest upstream vessel of the series contains reaction solvent, used for washing purposes and the like. The most downstream of the reaction vessels contain nucleotide reagents. At least the valves associated with vessels containing nucleotide reagents have two separate and discrete fluid flow passageways, the first of which is used exclusively for flow of reagent from its associated vessel into the fluid flow conduit, and the second of which is used exclusively for flow of solvent or reagents from an upstream vessel therethrough, thereby eliminating the possibilities of cross-contamination as a result of nucleotide reagent residuals left in the dead space of the valve.
Inventors
- 👤 Robert Bender
- 👤 Peter David Duck
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 14:53
CPC Classifications
C; C07; C07H; C07H21/00; B; B01; B01J; B01J19/00; B01J19/0046; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00281; B01J2219/00286; B01J2219/00497; B01J2219/005; B01J2219/00583; B01J2219/0059; B01J2219/00596; B01J2219/00718; B01J2219/0072; B01J2219/00722; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B60/00; C40B60/14; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/582; Y10; Y10S; Y10S435/00; Y10S435/82
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
151 words
Apparatus for the step-wise synthesis of polynucleotides, by sequential feed of appropriate nucleotide reagents and other reactants to a polymer-nucleoside or polymer-nucleotide solid complex for reaction therewith, the apparatus comprising: a reaction vessel for receiving the solid complex and conducting reactions therein; a solvent receiving reservoir upstream of said vessel; a fluid flow conduit extending from the solvent receiving reservoir to the reaction vessel; a plurality of nucleotide reagent receiving reservoirs; at least one reactant receiving reservoir; a plurality of two-way valyes serially arranged in said conduit, each one associated with the respective nucleotide reagent receiving reservoir; each said valve being disposable in a first position in which it provides fluid communication from its respective associated reservoir to said conduit, and in a second position in, which it provides for flow of fluid from another, upstream reservoir therethxough, and prevent flow of fluid from its respective associated reservoir to said conduit.
Claim 2
Dependent Claim
151 words
Apparatus for the step-wise synthesis of polynucleotides, by sequential feed of appropriate nucleotide reagents and other reactants to a polymer-nucleoside or polymer-nucleotide solid complex for reaction therewith, the apparatus comprising: a reaction vessel for receiving the solid complex and conducting reactions therein; a solvent receiving reservoir upstream of said vessel; a fluid flow conduit extending from the solvent receiving reservoir to the reaction vessel; a plurality of nucleotide reagent receiving reservoirs; at least one reactant receiving reservoir; a plurality of two-way valyes serially arranged in said conduit, each one associated with the respective nucleotide reagent receiving reservoir; each said valve being disposable in a first position in which it provides fluid communication from its respective associated reservoir to said conduit, and in a second position in, which it provides for flow of fluid from another, upstream reservoir therethxough, and prevent flow of fluid from its respective associated reservoir to said conduit.
Claim 3
Dependent Claim
16 words
Apparatus according to claim 1 wherein each said valve is biased towards to its second position.
Claim 4
Dependent Claim
27 words
Apparatus according to claim 1 or 2 further also including a pumping means for delivery of solvent, reagents and reactants to the reaction column, via said conduit.
Claim 5
Dependent Claim
15 words
Apparatus according to claim 3 wherein the pumping means is located downstream of the valves.
Claim 6
Dependent Claim
75 words
Apparatus according to any one of the preceding claims wherein each said valve has two exclusive and discrete flow paths therethrough, the first said flow path being utlised in the first position of the valve to provide fluid communication from its respective associated reservoir to the fluid flow conduit, and the second said flow path being utilised in the second position of the valve, to provide for flow of fluid from another, upstream reservoir therethrough.
Claim 7
Dependent Claim
37 words
Apparatus according to any one of the preceding claims further including interlock means for said valves arranged to ensure that no more than one of said valves is in its first said position at any given time.
Claim 8
Dependent Claim
32 words
Apparatus according to any one of the preceding claims further including a drain conduit from the downstream end of said column, to permit drain of solvent and spent reactants from said column.
Claim 9
Dependent Claim
118 words
Apparatus according to claim 7, wherein said drain conduit includes a plurality of serially arranged two-way recycle valves, each associated with a respective one of said nucleotide reagent receiving reservoirs, and adapted to provide selective recycle of unreacted nucleotide reagent from the column to its respective reservoir, and- each said recycle valve having two exclusive and discrete flow paths therethrough, disposable in a first position in which its first flow path is utilised to provide fluid communication from the drain conduit to the respective associated reservoir, and in a second position in which its second flow path is utlised to provide for flow of fluid from the column through the valve towards the drain, in a downstream direction.
Claim 10
Dependent Claim
107 words
Apparatus according to any one of the preceding claims including at least three reactant receiving reservoirs, each having an associated two-way valve serially arranged in said fluid flow conduit, each said two-way valve having two exclusive and discrete flow paths therethrough and being disposable in a first position in which first flow path is utilised to provide fluid communication from its respeetive associates reservoir to said conduit, and in a second position in which its second flow path is utlised to provide for flow of fluid from another, upstream reservoir through the valve in the downstream direction, and each said valve being biased towards its second position.
Claim 11
Dependent Claim
33 words
Apparatus according to claim 9 wherein the valves associated with the reactant receiving reservoirs are serially arranged in the fluid flow conduit upstream from the valves associated with the nucleotide reagent receiving reservoirs.
References Cited (Backward Citations)
No citation records indexed.
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