Patent EP0201205A1 successfully refreshed from Google Patents.
Patent EP0145338A1 successfully refreshed from Google Patents.
EP0145338A1
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EP
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Octopine T-DNA promoters
Assignee: Mycogen Plant Science Inc
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Filing: 1984-11-16
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Granted: —
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Expires: 2004-11-16
Abstract
Abstract The sequence of the T-DNA of the octopine-type Ti plasmid found in Agrobacterium tumefaciens ATCC 15955 is disclosed. Fourteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found. The use of promoters and polyadenylation sites from pTi15955 to control expression of foreign structural genes is taught, using as examples the structural genes for the Phaseolus vulgaris storage protein phaseolin, P. vulgaris lectin, thaumatin, and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.
Inventors
- 👤 Richard F. Barker
- 👤 John D. Kemp
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 12:27
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8279; C12N15/8286; C12N15/8216; Y; Y02; Y02A; Y02A40/00; Y02A40/10; Y02A40/146
Claims Analysis
Total Claims: 104
Claim 1
Independent Claim
95 words
A method of genetically modifying a plant cell by transforming the cell to contain a promoter, a foreign structural gene, and a polyadenylation site, the promoter, the structural gene, and the polyadenylation site being in such position and orientation with respect to one another that the structural gene is expressible in a plant cell under control of the promoter and the polyadenylation site at least one of the promoter and the polyadenylation site being obtained from any TIP gene, hybridizable to an oT-DNA gene selected from a group consisting of ORFs 1,3,4,5,8,9,10,18,19,21, 24,25, and 26.
Claim 2
Independent Claim
95 words
A method of genetically modifying a plant cell by transforming the cell to contain a promoter, a foreign structural gene, and a polyadenylation site, the promoter, the structural gene, and the polyadenylation site being in such position and orientation with respect to one another that the structural gene is expressible in a plant cell under control of the promoter and the polyadenylation site at least one of the promoter and the polyadenylation site being obtained from any TIP gene, hybridizable to an oT-DNA gene selected from a group consisting of ORFs 1,3,4,5,8,9,10,18,19,21, 24,25, and 26.
Claim 3
Dependent Claim
16 words
A method according to claim 1 wherein the TIP gene is from an octopine-type Ti plasmid.
Claim 4
Dependent Claim
12 words
A method according to claim 2 wherein the Ti plasmid is pTi15955.
Claim 5
Dependent Claim
28 words
A method according to claim 1 where the promoter, the structural gene, or the polyadenylation site comprises an insertion, deletion, or subsitution of one or more nucleotide pairs.
Claim 6
Dependent Claim
22 words
A method according to claim 1 wherein the structural gene conveys a phenotype of improved tolerance to extremes of heat or cold.
Claim 7
Dependent Claim
21 words
A method according to claim 1 wherein the structural gene conveys a phenotype of improved tolerance to drought or osmotic stress.
Claim 8
Dependent Claim
24 words
A method according to claim 1 wherein the structural gene conveys a phenotype of improved resistance or tolerance to insects, arachnids, nematodes, or epiphytes.
Claim 9
Dependent Claim
40 words
A method according to claim 7 wherein the structural gene encodes an insecticidal toxin identical to or derived from the crystal protein of Baci'"js thuringiensis and the oT-DNA gene is selected from a group consisting of ORFs 1,3,9,18,19,21,25, and 26.
Claim 10
Dependent Claim
24 words
A method according to claim 1 wherein the structural gene conveys a phenotype of improved resistance or tolerance to fungal, bacterial, or viral diseases.
Claim 11
Dependent Claim
23 words
A method according to claim 1 wherein the structural gene encodes an enzyme or is responsible for the production of novel secondary metabolites.
Claim 12
Dependent Claim
20 words
A method according to claim 1 wherein the structural gene conveys a phenotype of improved nutrition, flavor, or processing properties.
Claim 13
Dependent Claim
14 words
A method according to claim 11 wherein the structural gene encodes a storage protein.
Claim 14
Dependent Claim
17 words
A method according to claim 12 wherein the storage protein gene is hybridizable to a phaseolin gene.
Claim 15
Dependent Claim
14 words
A method according to claim 11 wherein the structural gene encodes a sweet protein.
Claim 16
Dependent Claim
17 words
A method according to claim 14 wherein the sweet protein is thaumatin or a precursor of thaumatin.
Claim 17
Dependent Claim
13 words
A method according to claim 1 wherein the structural gene encodes a lectin.
Claim 18
Dependent Claim
13 words
A method according to claim 16 wherein the lectin is a legume lectin.
Claim 19
Dependent Claim
21 words
A method according to claim 1 wherein the structural gene conveys a phenotype of a changed morphological trait or developmental pattern.
Claim 20
Dependent Claim
17 words
19 A method according to claim 1 wherein the structural gene conveys a phenotype of male sterility.
Claim 21
Dependent Claim
17 words
A method according to claim 1 wherein the structural gene conveys a phenotype of improved photosynthetic efficiency.
Claim 22
Dependent Claim
17 words
A method according to claim 1 wherein the structural gene conveys a phenotype of improved nitrogen fixation.
Claim 23
Dependent Claim
18 words
A method according to claim 1 wherein the structural gene conveys a phenotype of improved uptake of nutrients.
Claim 24
Dependent Claim
28 words
A method according to claim 1-wherein the structural gene conveys a phenotype of improved resistance or tolerance to herbicides, improved competition with other plants, or increased crop yield.
Claim 25
Dependent Claim
17 words
A method according to claim 1 wherein the structural gene conveys a phenotype of improved germplasm identification.
Claim 26
Dependent Claim
24 words
A method according to claim 1 wherein the structural gene conveys a phenotype of resistance in cell or tissue culture to a selective agent.
Claim 27
Dependent Claim
85 words
A DNA vector comprising a promoter, a foreign structural gene, and a polyadenylation site, the promoter, the structural gene, and the polyadenylation site being in such position and orientation with respect to one another that the structural gene is expressible in a plant cell under control of the promoter and the polyadenylation site, at least one of the promoter and the polyadenylation site being obtained from any TIP gene hybridizable to an oT-DNA gene selected from a group consisting of ORFs 1,3,4,5,8,9,10,18,19,21, 24,25, and 26.
Claim 28
Dependent Claim
85 words
A DNA vector comprising a promoter, a foreign structural gene, and a polyadenylation site, the promoter, the structural gene, and the polyadenylation site being in such position and orientation with respect to one another that the structural gene is expressible in a plant cell under control of the promoter and the polyadenylation site, at least one of the promoter and the polyadenylation site being obtained from any TIP gene hybridizable to an oT-DNA gene selected from a group consisting of ORFs 1,3,4,5,8,9,10,18,19,21, 24,25, and 26.
Claim 29
Dependent Claim
16 words
A vector according to claim 26 wherein the TIP gene is from an octopine-type Ti plasmid.
Claim 30
Dependent Claim
12 words
A vector according to claim 27 wherein the Ti plasmid is pTi15955.
Claim 31
Dependent Claim
27 words
A vector according to claim 26 wherein the promoter, the structural gene, or the polyadenylation site comprises an insertion, deletion, or substitution of one or more nucleotides.
Claim 32
Dependent Claim
22 words
A vector according to claim 26 wherein the structural gene conveys a phenotype of improved tolerance to extremes of heat or cold.
Claim 33
Dependent Claim
21 words
A vector according to claim 26 wherein the structural gene conveys a phenotype of improved tolerance to drought or osmotic stress.
Claim 34
Dependent Claim
24 words
A vector according to claim 26 wherein the structural gene conveys a phenotype of improved resistance or tolerance to insects, arachnids, nematodes, or epiphytes.
Claim 35
Dependent Claim
40 words
A vector according to claim 35 wherein the structural gene encodes an insecticidal toxin identical to or derived from the crystal protein of Bacillus thuringiensis and the oT-DNA gene is selected from a group consisting of ORFs 1,3,9,18,19,21,25, and 26.
Claim 36
Dependent Claim
24 words
A vector according to claim 26 wherein the structural gene conveys a phenotype of improved resistance or tolerance to fungal, bacterial, or viral diseases.
Claim 37
Dependent Claim
23 words
A vector according to claim 26 wherein the structural gene encodes an enzyme or is responsible for the production of novel secondary metabolites.
Claim 38
Dependent Claim
20 words
A vector according to claim 26 wherein the structural gene conveys a phenotype of improved nutrition, flavor, or processing properties.
Claim 39
Dependent Claim
14 words
A vector according to claim 36 wherein the structural gene encodes a storage protein.
Claim 40
Dependent Claim
16 words
A vector according to claim 37 wherein the storage protein is hybridizable to a phaseolin gene.
Claim 41
Dependent Claim
14 words
A vector according to claim 36 wherein the structural gene encodes a sweet protein.
Claim 42
Dependent Claim
17 words
A vector according to claim 39 wherein the sweet protein in thaumatin or a precursor of thaumatin.
Claim 43
Dependent Claim
13 words
A vector according to claim 36 wherein the structural gene encodes a lectin.
Claim 44
Dependent Claim
13 words
A vector according to claim 41 wherein the lectin is a legume lectin.
Claim 45
Dependent Claim
35 words
A vector according to claim 36 wherein the structural gene conveys a phenotype of a changed morphological trait, a changed developmental pattern, male sterility, improved photosynthetic efficiency, improved nitrogen fixation, or improved uptake of nutrients.
Claim 46
Dependent Claim
33 words
A vector according to claim 36 wherein the structural gene conveys a phenotype of improved resistance or tolerance to herbicides or improved competition with other plants, increased crop yield, or improved germplasm identification.
Claim 47
Dependent Claim
24 words
A vector according to claim 36 wherein the structural gene conveys a phenotype of resistance in cell or tissue culture to a selective agent.
Claim 48
Dependent Claim
91 words
A bacterial strain comprising a DNA vector wherein the vector comprises a promoter, a foreign structural gene, and a polyadenylation site, the promoter, the structural gene, and the polyadenylation site being in such position and orientation with respect to one another that the structural gene is expressible in a plant cell under control of the promoter and the polyadenylation site, at least one of the promoter and the polyadenylation site being obtained from any TIP gene hybridizable to an oT-DNA gene selected from a group consisting of ORFs 1,3,4,5,8,9,10,18,19,21,24,25, and 26.
Claim 49
Dependent Claim
91 words
A bacterial strain comprising a DNA vector wherein the vector comprises a promoter, a foreign structural gene, and a polyadenylation site, the promoter, the structural gene, and the polyadenylation site being in such position and orientation with respect to one another that the structural gene is expressible in a plant cell under control of the promoter and the polyadenylation site, at least one of the promoter and the polyadenylation site being obtained from any TIP gene hybridizable to an oT-DNA gene selected from a group consisting of ORFs 1,3,4,5,8,9,10,18,19,21,24,25, and 26.
Claim 50
Dependent Claim
14 words
A bacterial strain according to claim 46 wherein the Ti gene is from pTi15955.
Claim 51
Dependent Claim
108 words
A bacterial strain according to claim 46 wherein the structural gene conveys a phenotype of improved tolerance to extremes of heat or cold, improved tolerance to drought or osmotic stress, improved resistance or tolerance to insects, arachnids, nematodes, or epiphytes, improved resistance or tolerance to fungal, bacterial, or viral diseases, encoding an enzyme or being responsible for the production of novel secondary metabolites, improved nutrition, flavor, or processing properties, a changed morphological trait, a changed developmental pattern, male sterility, improved photosynthetic efficiency, improved nitrogen fixation, or improved uptake of nutrients, improved resistance or tolerance to herbicides, improved competition with other plants, increased crop yield, or improved germplasm identification.
Claim 52
Dependent Claim
41 words
A bacterial strain according to claim 48 wherein the structural gene encodes an insecticidal toxin identical to or derived from the crystal protein of Bacillus thuringiensis and the oT-DNA gene is selected from a group consisting of ORFs 1,3,9,18,19,21,25, and 26.
Claim 53
Dependent Claim
15 words
A bacterial strain according to claim 48 wherein the structural gene encodes a storage protein.
Claim 54
Dependent Claim
17 words
A bacterial strain according to claim 50 wherein the storage protein is hybridizable to a phaseolin gene.
Claim 55
Dependent Claim
15 words
A bacterial strain according to claim 48 wherein the structural gene encodes a sweet protein.
Claim 56
Dependent Claim
18 words
A bacterial strain according to claim 48 wherein the sweet protein in thaumatin or a precursor of thaumatin.
Claim 57
Dependent Claim
14 words
A bacterial strain according to claim 53 wherein the structural gene encodes a lectin.
Claim 58
Dependent Claim
14 words
A bacterial strain according to claim 54 wherein the lectin is a legume lectin.
Claim 59
Dependent Claim
24 words
A vector according to claim 46 wherein the structural gene conveys a phenotype of resistance in cell or tissue culture to a selective agent.
Claim 60
Dependent Claim
94 words
A plant tissue comprising a genetically modified plant cell transformed to contain a promoter, a foreign structural gene, and a polyadenylation site, the promoter, the structural gene, and the polyadenylation site being in such position and orientation with respect to one another that the structural gene is expressible in a plant cell under control of the promoter and the polyadenylation site, at least one of either the promoter and the polyadenylation site being obtained from any TIP gene hybridizable to an oT-DNA gene selected from a group consisting of ORFs 1,3,4,5,8,9,10,18, 19,21,24,25, and 26.
Claim 61
Dependent Claim
94 words
A plant tissue comprising a genetically modified plant cell transformed to contain a promoter, a foreign structural gene, and a polyadenylation site, the promoter, the structural gene, and the polyadenylation site being in such position and orientation with respect to one another that the structural gene is expressible in a plant cell under control of the promoter and the polyadenylation site, at least one of either the promoter and the polyadenylation site being obtained from any TIP gene hybridizable to an oT-DNA gene selected from a group consisting of ORFs 1,3,4,5,8,9,10,18, 19,21,24,25, and 26.
Claim 62
Dependent Claim
16 words
A tissue according to claim 57 wherein the TIP gene is from an octopine-type Ti plasmid.
Claim 63
Dependent Claim
13 words
A tissue according to claim 58 wherein the Ti plasmid is from pTi15955.
Claim 64
Dependent Claim
28 words
A tissue according to claim 57 wherein the promoter, the structural gene, or the polyadenylation site comprises an insertion, deletion, or substitution of one or more nucleotide pairs.
Claim 65
Dependent Claim
22 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of improved tolerance to extremes of heat or cold.
Claim 66
Dependent Claim
21 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of improved tolerance to drought or osmotic stress.
Claim 67
Dependent Claim
24 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of improved resistance or tolerance to insects, arachnids, nematodes, or epiphytes.
Claim 68
Dependent Claim
40 words
A tissue according to claim 63 wherein the structural gene encodes an insecticidal toxin identical to or derived from the crystal protein of Bacillus thuringiensis and the oT-DNA gene is selected from a group consisting of ORFs 1,3,9,18,19,21,25, and 26.
Claim 69
Dependent Claim
24 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of improved resistance or tolerance to fungal, bacterial, or viral diseases.
Claim 70
Dependent Claim
23 words
A tissue according to claim 57 wherein the structural gene encodes an enzyme or is responsible for the production of novel secondary metabolites.
Claim 71
Dependent Claim
20 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of improved nutrition, flavor, or processing properties.
Claim 72
Dependent Claim
14 words
A tissue according to claim 67 wherein the structural gene encodes a storage protein.
Claim 73
Dependent Claim
17 words
A tissue according to claim 68 wherein the storage protein gene is hybridizable to a phaseolin gene.
Claim 74
Dependent Claim
13 words
A tissueaccording to claim 67 wherein the structural gene encodes a sweet protein.
Claim 75
Dependent Claim
17 words
A tissue according to claim 70 wherein the sweet protein is thaumatin or a precursor of thaumatin.
Claim 76
Dependent Claim
13 words
A tissue according to claim 67 wherein the structural gene encodes a lectin.
Claim 77
Dependent Claim
21 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of a changed morphological trait or developmental pattern.
Claim 78
Dependent Claim
16 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of male sterility.
Claim 79
Dependent Claim
17 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of improved photosynthetic efficiency.
Claim 80
Dependent Claim
17 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of improved nitrogen fixation.
Claim 81
Dependent Claim
18 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of improved uptake of nutrients.
Claim 82
Dependent Claim
29 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of improved resistance or tolerance to herbicides, improved competition with other plants, or increased crop yield.
Claim 83
Dependent Claim
17 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of improved germplasm identification.
Claim 84
Dependent Claim
24 words
A tissue according to claim 57 wherein the structural gene conveys a phenotype of resistance in cell or tissue culture to a selective agent.
Claim 85
Dependent Claim
12 words
A tissue according to claim 57 wherein the plant is a gymnosperm.
Claim 86
Dependent Claim
12 words
A tissue according to claim 57 wherein the plant is a monocot.
Claim 87
Dependent Claim
12 words
A tissue according to claim 57 wherein the plant is a dicot.
Claim 88
Dependent Claim
91 words
A plant comprising a genetically modified plant cell transformed to contain a promoter, a foreign structural gene, and a polyadenylation site, the promoter, the structural gene, and the polyadenylation site being in such position and orientation with respect to one another that the structural gene is expressible in a plant cell under control of the promoter and the polyadenylation site, at least one of the promoter and the polyadenylation site being obtained from any TIP gene hybridizable to an oT-DNA gene selected from a group consisting of ORFs 1,3,4,5,8,9,10,18,19,21,24,25, and 26.
Claim 89
Dependent Claim
91 words
A plant comprising a genetically modified plant cell transformed to contain a promoter, a foreign structural gene, and a polyadenylation site, the promoter, the structural gene, and the polyadenylation site being in such position and orientation with respect to one another that the structural gene is expressible in a plant cell under control of the promoter and the polyadenylation site, at least one of the promoter and the polyadenylation site being obtained from any TIP gene hybridizable to an oT-DNA gene selected from a group consisting of ORFs 1,3,4,5,8,9,10,18,19,21,24,25, and 26.
Claim 90
Dependent Claim
13 words
A plant according to claim 84 wherein the T-DNA gene is from pTi15955.
Claim 91
Dependent Claim
28 words
A plant according to claim 84 wherein the promoter, the structural gene, or the polyadenylation site comprises an insertion, deletion, or substitution of one or more nucleotide pairs.
Claim 92
Dependent Claim
22 words
A plant according to claim 84 wherein the structural gene conveys a phenotype of improved tolerance to extremes of heat or cold.
Claim 93
Dependent Claim
21 words
A plant according to claim 84 wherein the structural gene conveys a phenotype of improved tolerance to drought or osmotic stress.
Claim 94
Dependent Claim
24 words
A plant according to claim 84 wherein the structural gene conveys a phenotype of improved resistance or tolerance to insects, arachnids, nematodes, or epiphytes.
Claim 95
Dependent Claim
33 words
A plant according to claim 89 wherein the structural gene encodes all or part of a crystal protein and the oT-DNA gene is selected from a group consisting of ORFs 1,3,9,18,19,21,25, and 26.
Claim 96
Dependent Claim
24 words
A plant according to claim 84 wherein the structural gene conveys a phenotype of improved resistance or tolerance to fungal, bacterial, or viral diseases.
Claim 97
Dependent Claim
23 words
A plant according to claim 85 wherein the structural gene encodes an enzyme or is responsible for the production of novel secondary metabolites.
Claim 98
Dependent Claim
20 words
A plant according to claim 84 wherein the structural gene conveys a phenotype of improved nutrition, flavor, or processing properties.
Claim 99
Dependent Claim
33 words
A plant according to claim 93 wherein the structural gene encodes all or part of a protein selected from a group consisting of seed storage proteins, plant lectins, thaumatin, and precursors of thaumatin.
Claim 100
Dependent Claim
35 words
A plant according to claim 84 wherein the structural gene conveys a phenotype of a changed morphological trait, a changed developmental pattern, male sterility, improved photosynthetic efficiency, improved nitrogen fixation, or improved uptake of nutrients.
Claim 101
Dependent Claim
32 words
A plant according to claim 84 wherein the structural gene conveys a phenotype of improved resistance or tolerance to herbicides, improved competition with other plants increased crop yield, or improved germplasm identification.
Claim 102
Dependent Claim
24 words
A plant according to claim 84 wherein the structural gene conveys a phenotype of resistance in cell or tissue culture to a selective agent.
Claim 103
Dependent Claim
12 words
A plant according to claim 84 wherein the plant is a gymnosperm.
Claim 104
Dependent Claim
25 words
A plant according to claim 84 wherein the plant is a monocot. 100. A plant according to claim 84 wherein the plant is a dicot.
References Cited (Backward Citations)
EP0099255A2
Cited
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