EP0099255A2
Expired / Lapsed
EP
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Method for transforming plant cells
Assignee: International Paper Co
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Filing: 1983-07-08
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Granted: —
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Expires: 2003-07-08
Abstract
Abstract A method for transforming a plant cell in vitro with a Ti plasmid. The method involves inoculating the plant cell with a strain of Agrobacterium tumefaciens, containing the Ti plasmid, in the presence of an opine metabolite, that is normally synthesized only by a plant cell which has already been transformed by the Ti plasmid, or in the presence of the precursors of the opine metabolite. However, the opine metabolite or its precursors, which are used, must also be able to induce conjugative activity of the Ti plasmid.
Inventors
- 👤 Linda M. Cello
- 👤 William L. Olsen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 12:33
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8201; C12N15/8202; C12N15/8205
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
81 words
1-. A method for transforming a plant cell in vitro with a Ti plasmid, comprising: inoculating the plant cell with a strain of Agrobacterium tumefaciens, containing the Ti plasmid, in the presence of an opine metabolite, that is normally synthesized only by a plant cell which has already been transformed by the Ti plasmid, or in the presence of the precursors of the opine metabolite; said opine metabolite or the precursors being capable of inducing conjugative activity of said Ti plasmid.
Claim 2
Dependent Claim
81 words
1-. A method for transforming a plant cell in vitro with a Ti plasmid, comprising: inoculating the plant cell with a strain of Agrobacterium tumefaciens, containing the Ti plasmid, in the presence of an opine metabolite, that is normally synthesized only by a plant cell which has already been transformed by the Ti plasmid, or in the presence of the precursors of the opine metabolite; said opine metabolite or the precursors being capable of inducing conjugative activity of said Ti plasmid.
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein the Ti plasmid is an octopine Ti plasmid
Claim 4
Dependent Claim
29 words
3'. The method of claim 2 wherein the plant cell is inoculated in the presence of octopine or in the presence of arginine and pyruvate as the octopine metabolites.
Claim 5
Dependent Claim
22 words
The method of claim 3 wherein the plant cell is inoculated in the presence of arginine and pyruvate as the octopine metabolites.
Claim 6
Dependent Claim
27 words
The method of claim 1 wherein the plant cell is inoculated in the presence of lysopine and octopinic acid as the opine metabolites or ornithine and pyruvate.
Claim 7
Dependent Claim
16 words
The method according to claim 1 wherein the Ti plasmid has inserted therein a DNA sequence.
References Cited (Backward Citations)
No citation records indexed.
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